GI | Gigantea (affinity purified)

PRODUCT INFORMATION IN PDF

product information Background GI (GIGANTEA) is involved in several developmental processes including regulation of circadian rhythm and photoperiodic flowering, phytochrome B signaling, circadian clock, carbohydrate metabolism and cold stress response. Immunogen KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana GI, UniProt:Q9SQI2,TAIR: AT1G22770 Host Rabbit Clonality Polyclonal Clone Purity Affinity purified serum in PBS, pH 7.4 Format Lyophilized in PBS pH 7.4 Quantity 50 µg Reconstitution For reconstitution add 50 µl of sterile water. Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications Western blot (WB) Related products AS16 3160 | anti-CO | Constans, rabbit polyclonal antibody AS12 1864 | anti-GI | Gigantea, rabbit polyclonal antibody AS12 2646 | anti-TOC1 | Timing of CAB expression 1, rabbit polyclonal antibody AS14 2823 | anti-TOC1 | Timing of CAB expression 1 (other species), rabbit polyclonal antibody AS06 198 | anti-FT/TSF | Flowering locus T and twin sister of FT, rabbit polyclonal antibody AS12 1856 | anti-SOC1 | Suppressor of constans overexpression 1, rabbit polyclonal antibody Plant protein extraction buffer Secondary antibodies Additional information

application information
Recommended dilution		1 : 1000 (WB)
Expected | apparent MW		127.9 kDa
Confirmed reactivity		Arabidopsis thaliana
Predicted reactivity		Brassica campestris, Chrysanthemum morifolium, Dimocarpus longan, Festuca patensis, Gentiana triflora, Glycine soja, Hordeum vulgare, Liriodendron tulipifera, Lolium perenne, Lotus japonicus, Medicago truncatula, Plantago major, Populus balsamifera, Prunus dulcius, Ricinus communis, Secale cereale, Theobroma cacao, Triticum aestivum
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references		to be added when available, antibody released in January 2016.

application example



50 µg of total protein from Arabidopsis thaliana extracted with TRIZOL protocol and finally dissolved in buffer E (Martínez-García et al., 1999, Plant J 20:251-7), was denatured with SDS at 95 C for 5 min, were separated on 12% (w/v) acrylamide/bis-acrylamide SDS-PAGE and blotted 10 mins to nitrocellulose using semi-dry tank transfer. Blots were blocked with 5% (w/v) skimmed milk in TBSt (Tris-Buffer Saline + 0.1% (v/v) tween-20) for 2h at room temperature (RT) with agitation. TBSt Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed 4 times for 15 min in TBSt at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in TBSt for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL (Life Science). Exposure time was continuous for 10 mins in a CCD camera. The image was taken after 5 min exposure.

Courtesy of Dr. Federico Valverde Albacete, CSIC, Spain

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Plant protein extraction buffer

Secondary antibodies