GI | Gigantea (affinity purified)

AS12 1864A | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Product Information

Immunogen KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana GI, UniProt:Q9SQI2,TAIR: AT1G22770

Host Rabbit

Clonality Polyclonal

Purity Affinity purified serum in PBS, pH 7.4

Format Lyophilized in PBS pH 7.4

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW

127.9 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Brassica campestris, Chrysanthemum morifolium, Dimocarpus longan, Festuca patensis, Gentiana triflora, Glycine soja, Hordeum vulgare, Liriodendron tulipifera, Lolium perenne, Lotus japonicus, Medicago truncatula, Plantago major, Populus balsamifera, Prunus dulcius, Ricinus communis, Secale cereale, Theobroma cacao, Triticum aestivum
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples application example

western blot using anti-GI antibodies

50 µg of total protein from Arabidopsis thaliana extracted with TRIZOL protocol and finally dissolved in buffer E (Martínez-García et al., 1999, Plant J 20:251-7), was denatured with SDS at 95 C for 5 min, were separated on 12% (w/v) acrylamide/bis-acrylamide SDS-PAGE and blotted 10 mins to nitrocellulose using semi-dry tank transfer. Blots were blocked with 5% (w/v) skimmed milk in TBSt (Tris-Buffer Saline + 0.1% (v/v) tween-20) for 2h at room temperature (RT) with agitation. TBSt Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed 4 times for 15 min in TBSt at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in TBSt for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL (Life Science). Exposure time was continuous for 10 mins in a CCD camera. The image was taken after 5 min exposure.

Courtesy of Dr. Federico Valverde Albacete, CSIC, Spain

Additional information

Related products

Plant protein extraction buffer

Secondary antibodies

Background

Background GI (GIGANTEA) is involved in several developmental processes including regulation of circadian rhythm and photoperiodic flowering, phytochrome B signaling, circadian clock, carbohydrate metabolism and cold stress response.