GI | Gigantea (affinity purified)
AS12 1864A | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
DATA SHEET IN PDF| Data sheet | Product citations | Protocols | Add review |
Product Information
Immunogen
KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana GI, UniProt:Q9SQI2,TAIR: AT1G22770
Host
Rabbit
Clonality
Polyclonal
Purity
Affinity purified serum in PBS, pH 7.4
Format
Lyophilized in PBS pH 7.4
Quantity
50 ĩg
Reconstitution
For reconstitution add 50 ĩl of sterile water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
127.9 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Brassica campestris, Chrysanthemum morifolium, Dimocarpus longan, Festuca patensis, Gentiana triflora, Glycine soja, Hordeum vulgare, Liriodendron tulipifera, Lolium perenne, Lotus japonicus, Medicago truncatula, Plantago major, Populus balsamifera, Prunus dulcius, Ricinus communis, Secale cereale, Theobroma cacao, Triticum aestivum
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Application examples
application example
50 µg of total protein from Arabidopsis thaliana extracted with TRIZOL protocol and finally dissolved in buffer E (Martínez-García et al., 1999, Plant J 20:251-7), was denatured with SDS at 95 C for 5 min, were separated on 12% (w/v) acrylamide/bis-acrylamide SDS-PAGE and blotted 10 mins to nitrocellulose using semi-dry tank transfer. Blots were blocked with 5% (w/v) skimmed milk in TBSt (Tris-Buffer Saline + 0.1% (v/v) tween-20) for 2h at room temperature (RT) with agitation. TBSt Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed 4 times for 15 min in TBSt at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in TBSt for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL (Life Science). Exposure time was continuous for 10 mins in a CCD camera. The image was taken after 5 min exposure.
Courtesy of Dr. Federico Valverde Albacete, CSIC, Spain
50 µg of total protein from Arabidopsis thaliana extracted with TRIZOL protocol and finally dissolved in buffer E (Martínez-García et al., 1999, Plant J 20:251-7), was denatured with SDS at 95 C for 5 min, were separated on 12% (w/v) acrylamide/bis-acrylamide SDS-PAGE and blotted 10 mins to nitrocellulose using semi-dry tank transfer. Blots were blocked with 5% (w/v) skimmed milk in TBSt (Tris-Buffer Saline + 0.1% (v/v) tween-20) for 2h at room temperature (RT) with agitation. TBSt Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed 4 times for 15 min in TBSt at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in TBSt for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL (Life Science). Exposure time was continuous for 10 mins in a CCD camera. The image was taken after 5 min exposure.
Courtesy of Dr. Federico Valverde Albacete, CSIC, Spain
Additional information
Background
Background
GI (GIGANTEA) is involved in several developmental processes including regulation of circadian rhythm and photoperiodic flowering, phytochrome B signaling, circadian clock, carbohydrate metabolism and cold stress response.
Product citations
Related products: GI | Gigantea (affinity purified)
AS16 ECL-S | extreme low femtogram detection
- 10ml trial size is restricted to one unit per cust...
- 10ml trial size is restricted to one unit per cust...
From 15 €
AS06 198 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
292 €
Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
Off...
Off...
194 €
AS12 1856 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
292 €
Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items
Agrisera AB Box 57, SE-911 21 Vännäs, SWEDEN | Phone: +46(0)935 33000 | Fax: +46(0)935 33044 | 
