SOC1 | Suppressor of constans overexpression 1

AS12 1856 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

SOC1 | Suppressor of constans overexpression 1 in the group Antibodies Plant/Algal  / DNA/RNA/Cell Cycle / Transcription regulation at Agrisera AB (Antibodies for research) (AS12 1856)
SOC1 | Suppressor of constans overexpression 1


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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen KLH-conjugated synthetic peptidederived from Arabidpsis thaliana SOC1. UniProt: O64645,TAIR: AT2G45660
Host Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW

24 kDa


Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Brassica sp., Cardamine sylvatica, Sinapsis juncea
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples

application example

2 µg of Arabidopsis thaliana total protein from 15 days old seedlings (1) from 200ng/μl extracted with buffer containing Tris (pH7.5), NaCl, Triton-x100 and protease inhibitors and yeast protein extracts from SG335 cells transformed with pGADT7/SOC1 construct (2) were separated on 12 % SDS-PAGE and blotted 40 min. to PVDF. Blots were blocked  with 5% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody  at a dilution of 1:5000 for 1.5h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation.  Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 45min at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 7 min. 

Double band in the yeast sample might be due to post-translational mofifications as experiment with yeast harbouring vector showed no cross-reactivity.

Courtesy of Dr Theoni Margaritopoulou, Agricultural University of Athens, Greece


western blot using anti-SOC1 antibodies

Additional information

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Plant protein extraction buffer

Secondary antibodies


Background SOC1 (Suppressor of constans overexpression 1) is a transcription activator active in flowering time control. Located in nucleus and cytoplasm. Widely expressed but not found in the apical meristem of short-day grown plants in vegetative stage.  Alternative names: AGAMOUS-LIKE 25, AGL25, FLC, FLF, FLOWERING LOCUS C, FLOWERING LOCUS F.

Product citations

Selected references Cuerda-Gil et al. (2022) A plant tethering system for the functional study of protein-RNA interactions in vivo. Plant Methods. 2022 Jun 4;18(1):75. doi: 10.1186/s13007-022-00907-w. PMID: 35658900; PMCID: PMC9166424.

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