SOC1 | Suppressor of constans overexpression 1

Product no: AS12 1856

AS12 1856 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen: KLH-conjugated synthetic peptidederived from Arabidpsis thaliana SOC1. UniProt: O64645,TAIR: AT2G45660
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 g
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 5000 (WB)
    Expected | apparent MW:

    24 kDa

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Brassica sp., Cardamine sylvatica, Sinapsis juncea
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • application example

    2 µg of Arabidopsis thaliana total protein from 15 days old seedlings (1) from 200ng/μl extracted with buffer containing Tris (pH7.5), NaCl, Triton-x100 and protease inhibitors and yeast protein extracts from SG335 cells transformed with pGADT7/SOC1 construct (2) were separated on 12 % SDS-PAGE and blotted 40 min. to PVDF. Blots were blocked  with 5% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody  at a dilution of 1:5000 for 1.5h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation.  Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 45min at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 7 min. 

    Double band in the yeast sample might be due to post-translational mofifications as experiment with yeast harbouring vector showed no cross-reactivity.

    Courtesy of Dr Theoni Margaritopoulou, Agricultural University of Athens, Greece



     

    western blot using anti-SOC1 antibodies
  • Background
  • Background: SOC1 (Suppressor of constans overexpression 1) is a transcription activator active in flowering time control. Located in nucleus and cytoplasm. Widely expressed but not found in the apical meristem of short-day grown plants in vegetative stage.  Alternative names: AGAMOUS-LIKE 25, AGL25, FLC, FLF, FLOWERING LOCUS C, FLOWERING LOCUS F.
  • Product Citations
  • Selected references: Cuerda-Gil et al. (2022) A plant tethering system for the functional study of protein-RNA interactions in vivo. Plant Methods. 2022 Jun 4;18(1):75. doi: 10.1186/s13007-022-00907-w. PMID: 35658900; PMCID: PMC9166424.
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