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SOC1 | Suppressor of constans overexpression 1

AS12 1856 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

SOC1 | Suppressor of constans overexpression 1 in the group Plant/Algal Antibodies / Developmental Biology / Flowering at Agrisera AB (Antibodies for research) (AS12 1856)

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product information
Background SOC1 (Suppressor of constans overexpression 1) is a transcription activator active in flowering time control. Located in nucleus and cytoplasm. Widely expressed but not found in the apical meristem of short-day grown plants in vegetative stage.  Alternative names: AGAMOUS-LIKE 25, AGL25, FLC, FLF, FLOWERING LOCUS C, FLOWERING LOCUS F.
Immunogen KLH-conjugated synthetic peptidederived from Arabidpsis thaliana SOC1. UniProt: O64645,TAIR: AT2G45660
Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
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Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW

24 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Brassica sp., Cardamine sylvatica, Sinapsis juncea
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references

to be added when available, antibody released in December 2013.


application example

2 µg of Arabidopsis thaliana total protein from 15 days old seedlings (1) from 200ng/μl extracted with buffer containing Tris (pH7.5), NaCl, Triton-x100 and protease inhibitors and yeast protein extracts from SG335 cells transformed with pGADT7/SOC1 construct (2) were separated on 12 % SDS-PAGE and blotted 40 min. to PVDF. Blots were blocked  with 5% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody  at a dilution of 1:5000 for 1.5h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation.  Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 45min at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 7 min. 

Double band in the yeast sample might be due to post-translational mofifications as experiment with yeast harbouring vector showed no cross-reactivity.

Courtesy of Dr Theoni Margaritopoulou, Agricultural University of Athens, Greece



 

western blot using anti-SOC1 antibodies

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