COP1 | E3 ubiquitin-protein ligase COP1

Product no: AS20 4399

AS20 4399  | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen: His-tagged recombinant part of COP1 protein from Arabidopsis thaliana, overexpressed in E.coli, UniProt: P43254, TAIR: AT2G32950
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Antigen affinity purified serum, in PBS pH 7.4
    Format: Lyophilized
    Quantity: 50 µg
    Reconstitution: For reconstitution add 50 µl, of sterile water.
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 75 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Brassica napus, Brassica rapa, Camelina sativa, Capsella rubella, Eutrema salsugineum, Hirschfeldia incana, Raphanus sativus

    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Western blot with anti-COP1 antibodies

    Samples:
    Marker: Precision Plus Protein Dual Color Standards (Biorad, #1610394)​
    1 - Arabidopsis thaliana cop1-4 mutant​
    2 - Arabidopsis thaliana 35S-FLAG-GFP-COP1/cop1-4 transgenic line #1​
    3 - Arabidopsis thaliana 35S-FLAG-GFP-COP1/cop1-4 transgenic line #2​ ​

    Total protein extracted freshly from 15 seedlings (7-day-old grown under short day condition with 8h light/16h dark) with 2x Laemmli sample buffer (Biorad, 120 mM Tris-HCl pH 6.8, 4% SDS, 20% glycerol, 0.02% bromophenol blue, 200 Mm dithiothreitol), and then denatured at 95°C for 10 min. Proteins were separated on pre-cast SDS-polyacylamide gels with a 7.5% acrylamide and blotted 7 min to nitrocellulose membranes (Biorad Trans-Blot Turbo RTA Nitrocellulose Transfer Kit), using semi-dry transfer. Blot was blocked with EveryBlot Blocking Buffer (Biorad) for 10 min at RT with agitation. Bolt was incubated in the anti-COP1 antibody at a dilution of 1:1000 for 4 °C/ON with agitation. The primary antibody solution was decanted, and the membrane was first washed briefly once (5 s) and then for 15 min, followed by 3 additional washings of 5 min in 1x TBS-T (without blocking agent) with agitation. Blot was incubated in Agrisera matching secondary antibody (Goat anti-Rabbit IgG, HRP conjugated, AS09 602) diluted to 1:25000 for 1h at RT with agitation). The blot was washed as above and developed for 2~3 min with chemiluminescent detection (AgriseraSuperBright, AS16 ECL-S-10), according to the manufacture’s instructions. The Exposure time was 20 seconds.​

  • Background
  • Background: COP1 (E3 ubiquitin-protein ligase COP1) is onvolved in proteasome-mediated ubiquitin-dependent protein catabolic process and protein ubiquitination. The protein contains a ring finger zinc-binding motif, a coiled-coil domain, and several WD-40 repeats, similar to G-beta proteins. Acts as a repressor of photomorphogenesis and as an activator of etiolation in darkness. Represses photomorphogenesis in darkness by mediating ubiquitination and subsequent proteasomal degradation of light-induced transcription factors such as HY5, HYH and LAF1. Localized in the nucleus in darkness but is gradually relocated to the cytoplasm upon illumination.

    Alternative names: Constitutive photomorphogenesis protein 1, RING-type E3 ubiquitin transferase COP1
  • Product Citations
  • Selected references: 38221900
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Poster Collection
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AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

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AS12 1867 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items
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AS16 ECL-S-N-10 | Low pico to mid femtogram and extreme low femtogram detection

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