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CPK1 | Calcium-dependent protein kinase 1

AS19  4315  | Clonality: Polyclonal  |  Host: Rabbit   | Reactivity: Arabidopsis thaliana

CPK1 | Calcium-dependent protein kinase 1 in the group Antibodies for Plant/Algal  / Arabidopsis thaliana  at Agrisera AB (Antibodies for research) (AS19 4315)

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Product Information

Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana CPK1 protein sequence, UniProt: Q06850-1, TAIR AT5G04870
Host

Rabbit

Clonality Polyclonal
Purity Affinity purified serum, in PBS pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl, of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW 68 | 72 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Noccaea caerulescen, Triticum sp.
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example


Western blot using anti-CPK1 antibodies


Plant age: 4 weeks under short-day conditions (8 h light).
20 µg of the total protein from Arabidopsis thaliana plants,  (genetic background: Col-0) plants, wild-type (1), cpk1 (SALK_096452) (2), cpk1 (SALK_080155c) (3) were extracted with protein extraction buffer (Durian et al., 2019) and denatured in Laemmli-sample buffer (final concentrations: 5% (v/v) beta-mercaptoethanol, 69 mM Tris-HCl pH 6.8, 11.1% (v/v) glycerol, 2.15 % (v/v) SDS) for 10 min at 74 ºC. Samples were separated by discontinuous gel electrophoresis on a 10 % (w/v) separation SDS-PA-gel and blotted 1h to PVDF-membrane using a semi-dry system. The blot was blocked with 5% milk powder in TBS-T with agitation for 1h at room temperature (RT). After rinsing in TBS-T, the blot was incubated in the primary antibody at a dilution of 1:1000 in TBS-T with 1% milk powder in 7°C with agitation overnight. The antibody solution was decanted and the blot was rinsed briefly twice with TBS-T, then washed three times for 10 min in TBS-T at RT with agitation. The blot was incubated with secondary antibody (Anti-rabbit IgG horse radish peroxidase) diluted to 1:10 000 for 1h at RT in TBS-T with 1% milk-powder with agitation. The blot was washed as above plus once for 10 min with TBS and developed for 5 min with chemiluminescent detection reagent, according to manufacture's recommendations. Visualization by exposure to Super RX film (Fujifilm 47410-19236). Exposure time of the film: 30 min.

Courtesy of Dr. Guido Durian, University of Turku, Finland

Additional information

Related products

Background

Background CPK1 (CALCIUM-DEPENDENT PROTEIN KINASE1) is a calcium-dependent protein kinase that can phosphorylate phenylalanine ammonia lyase (PAL), a key enzyme in pathogen defense. 

Alternative names: AtCDPK 1, CDPK 1, Calcium-dependent protein kinase isoform AK1,

Product citations

Selected references Durian et al. (2019). PROTEIN PHOSPHATASE 2A-B'Ã?Âģ controls Botrytis cinerea resistance and developmental leaf senescence. Plant Physiol. 2019 Oct 28. pii: pp.00893.2019. doi: 10.1104/pp.19.00893.
Durian et al. (2019). PROTEIN PHOSPHATASE 2A-B'Ã?Âģ controls Botrytis cinerea resistance and developmental leaf senescence. Plant Physiol. 2019 Oct 28. pii: pp.00893.2019. doi: 10.1104/pp.19.00893.

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