CPK1 | Calcium-dependent protein kinase 1

Product no: AS19 4315

AS19 4315  | Clonality: Polyclonal  |  Host: Rabbit   | Reactivity: Arabidopsis thaliana

Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items
302
Add to cart
Delivery:  3-6 business days
  • Product Info
  • Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana CPK1 protein sequence, UniProt: Q06850-1, TAIR AT5G04870
    Host:

    Rabbit

    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 g
    Reconstitution: For reconstitution add 50 l, of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 68 | 72 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Noccaea caerulescen, Triticum sp.
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example


    Western blot using anti-CPK1 antibodies


    Plant age: 4 weeks under short-day conditions (8 h light).
    20 µg of the total protein from Arabidopsis thaliana plants,  (genetic background: Col-0) plants, wild-type (1), cpk1 (SALK_096452) (2), cpk1 (SALK_080155c) (3) were extracted with protein extraction buffer (Durian et al., 2019) and denatured in Laemmli-sample buffer (final concentrations: 5% (v/v) beta-mercaptoethanol, 69 mM Tris-HCl pH 6.8, 11.1% (v/v) glycerol, 2.15 % (v/v) SDS) for 10 min at 74 ºC. Samples were separated by discontinuous gel electrophoresis on a 10 % (w/v) separation SDS-PA-gel and blotted 1h to PVDF-membrane using a semi-dry system. The blot was blocked with 5% milk powder in TBS-T with agitation for 1h at room temperature (RT). After rinsing in TBS-T, the blot was incubated in the primary antibody at a dilution of 1:1000 in TBS-T with 1% milk powder in 7°C with agitation overnight. The antibody solution was decanted and the blot was rinsed briefly twice with TBS-T, then washed three times for 10 min in TBS-T at RT with agitation. The blot was incubated with secondary antibody (Anti-rabbit IgG horse radish peroxidase) diluted to 1:10 000 for 1h at RT in TBS-T with 1% milk-powder with agitation. The blot was washed as above plus once for 10 min with TBS and developed for 5 min with chemiluminescent detection reagent, according to manufacture's recommendations. Visualization by exposure to Super RX film (Fujifilm 47410-19236). Exposure time of the film: 30 min.

    Courtesy of Dr. Guido Durian, University of Turku, Finland

  • Background
  • Background: CPK1 (CALCIUM-DEPENDENT PROTEIN KINASE1) is a calcium-dependent protein kinase that can phosphorylate phenylalanine ammonia lyase (PAL), a key enzyme in pathogen defense. 

    Alternative names: AtCDPK 1, CDPK 1, Calcium-dependent protein kinase isoform AK1,
  • Product Citations
  • Selected references: Durian et al. (2019). PROTEIN PHOSPHATASE 2A-B'?³ controls Botrytis cinerea resistance and developmental leaf senescence. Plant Physiol. 2019 Oct 28. pii: pp.00893.2019. doi: 10.1104/pp.19.00893.
    Durian et al. (2019). PROTEIN PHOSPHATASE 2A-B'?³ controls Botrytis cinerea resistance and developmental leaf senescence. Plant Physiol. 2019 Oct 28. pii: pp.00893.2019. doi: 10.1104/pp.19.00893.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Poster Collection
  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
    Your name will be displayed as the sender.
    Number of reviews: (0)

    This product doesn't have any reviews.

Accessories

AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

201 €
Add to cart
Info

AS15 2831  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Chlamydomonas reinhardtii, Chlorococcum dorsiventrale

302 €
Add to cart
Info
Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items

AS13 2682 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

302 €
Add to cart
Info
Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items
Close