ATG4 | Autophagy protein 4 (algal)

Product no: AS15 2831

AS15 2831  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Chlamydomonas reinhardtii, Chlorococcum dorsiventrale

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  • Product Info
  • Immunogen: recombiant ATG4 of Chlamydomonas reinhardtii, Gene ID Cre12.g510100
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 µl
    Reconstitution: For reconstitution add 50 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 5000 (WB)
    Expected | apparent MW: 64,2 | 70 kDa
  • Reactivity
  • Confirmed reactivity: Chlamydomonas reinhardtii, Chlorococcum dorsiventrale
    Predicted reactivity:
    Coccomyxa subellipsoidea C-169, Tetraselmis sp. GSL018, Volvox carteri

    Species of your interest not listed? Contact us
    Not reactive in: Arabidopsis thaliana, Capsicum annuum, Nicotiana tabacum
  • Application Examples
  • Application example

    western blot using anti-ATG4 antibodies
    10 ng of recombinant, purified Chlamydomonas reinhardtii ATG4 (left) and 15 µg of Chlamydomonas reinhardtii total cell extract (right) were separated on 12 % SDS-PAGE and blotted 1h to nitrocellulose membrane using semi-dry transfer. Blots were blocked with 5 % dry milk in PBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:5000 over night at 4 ºC with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602, Agrisera) diluted to 1:10000 in 5 % dry milk for 1h at RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection reagent, according to the manufacturer's instructions. Exposure time was 30 seconds

    Courtesy of María Esther Pérez-Pérez and Jose L. Crespo, CSIC, Spain
  • Additional Information
  • Additional information:

    This antibody is recognizing 25 ng of recombinant CrATG4

    This product can be sold containing ProClin if requested.

    Additional information (application):
    Technical details how to work with this antibody are provided here: Pérez-Pérez et al. (2016). Control of Autophagy in Chlamydomonas Is Mediated through Redox-Dependent Inactivation of the ATG4 Protease. Plant Physiol. 2016 Dec;172(4):2219-2234.
  • Background
  • Background:
    ATG4 (Autophagy protein 4) is a protein with cyteine-type endopeptidase activity involved in autophagy process.
  • Product Citations
  • Selected references: Chen et al. (2016). The role of nitric oxide signalling in response to salt stress in Chlamydomonas reinhardtii. Planta. 2016 Sep;244(3):651-69. doi: 10.1007/s00425-016-2528-0. Epub 2016 Apr 26.
    Pérez-Pérez et al. (2016). Control of Autophagy in Chlamydomonas Is Mediated through Redox-Dependent Inactivation of the ATG4 Protease. Plant Physiol. 2016 Dec;172(4):2219-2234.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts
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