Name:
Phone:
E-mail:
Message


 


ATG4 | Autophagy protein 4 (algal)

AS15 2831  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Chlamydomonas reinhardtii

ATG4 | Autophagy protein 4 (algal) in the group Antibodies for Plant/Algal  / Protein Modifications / Autophagy-related and Ubiquitin-like Proteins at Agrisera AB (Antibodies for research) (AS15 2831)

DATA SHEET IN PDF

Qty: 
286
Buy 2 items of this product for 214.00 €/items
Buy 3 items of this product for 195.00 €/items
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen recombiant ATG4 of Chlamydomonas reinhardtii, Gene ID Cre12.g510100
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW

64.2 | 70 kDa

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii
Predicted reactivity
Coccomyxa subellipsoidea C-169, Tetraselmis sp. GSL018, Volvox carteri

Species of your interest not listed? Contact us
Not reactive in Arabidopsis thaliana, Capsicum annuum, Nicotiana tabacum

Application examples

Application examples Application example

western blot using anti-ATG4 antibodies
10 ng of recombinant, purified Chlamydomonas reinhardtii ATG4 (left) and 15 µg of Chlamydomonas reinhardtii total cell extract (right) were separated on 12 % SDS-PAGE and blotted 1h to nitrocellulose membrane using semi-dry transfer. Blots were blocked with 5 % dry milk in PBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:5000 over night at 4 ºC with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602, Agrisera) diluted to 1:10000 in 5 % dry milk for 1h at RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection reagent, according to the manufacturer's instructions. Exposure time was 30 seconds

Courtesy of María Esther Pérez-Pérez and Jose L. Crespo, CSIC, Spain

Additional information

Additional information

This antibody is recognizing 25 ng of recombinant CrATG4

This product can be sold containing ProClin if requested.

Technical details how to work with this antibody are provided here: Pérez-Pérez et al. (2016). Control of Autophagy in Chlamydomonas Is Mediated through Redox-Dependent Inactivation of the ATG4 Protease. Plant Physiol. 2016 Dec;172(4):2219-2234.

Related products

Related products AS19 4275 | Anti-ATG3 | Autophagy-related protein 3, rabbit antibodies
AS15 2831 | Anti-ATG4 | Autophagy protein 4, rabbit antibodies
AS19 4276 | Anti-ATG5 | Autophagy protein 5, rabbit antibodies
AS19 4277 | Anti-ATG7 | Autophagy-related protein 7, rabbit anitbodies

AS14 2811 | Anti-ATG8A | Autophagy-related protein 8a, rabbit antibodies
AS14 2769PRE | ATG8 | Autophagy-related protein, pre-immune serum

AS14 2769 | Anti-ATG8 | Autophagy-related protein, rabbit antibodies

AS16 4071 | Anti-ATG9 | Autophagy-related protein 9 (N-terminal), rabbit antibodies
AS16 4072 | Anti-ATG9 | Autophagy-related protein 9 (C-terminal), rabbit antibodies

AS19 4278 | Anti-ATG12b | Autophagy-related protein 12b, rabbit antibodies
AS19 4279 | Anti-ATG13a | Autophagy-related protein 13a, rabbit antibodies
AS19 4280 | Anti-ATG16 | Autophagy-related protein 16, rabbit antibodies

AS14 2805 | Anti-NBR1 | Autophagy substrate NBR1, rabbit antibodies
AS19 4281 | Anti-NBR1 | Autophagy substrate NBR1, rabbit antibodies

Background

Background
ATG4 (Autophagy protein 4) is a protein with cyteine-type endopeptidase activity involved in autophagy process.

Product citations

Selected references Chen et al. (2016). The role of nitric oxide signalling in response to salt stress in Chlamydomonas reinhardtii. Planta. 2016 Sep;244(3):651-69. doi: 10.1007/s00425-016-2528-0. Epub 2016 Apr 26.
Pérez-Pérez et al. (2016). Control of Autophagy in Chlamydomonas Is Mediated through Redox-Dependent Inactivation of the ATG4 Protease. Plant Physiol. 2016 Dec;172(4):2219-2234.

Related products: ATG4 | Autophagy protein 4 (algal)

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
From 15 €
AS16 ECL-S | extreme low femtogram detection
- 10ml trial size is restricted to one unit per cust...
From 10 €
AS15 3060 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
211 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
190 €