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CPK-EF | Calcium-dependent protein kinase EF domain

AS11 1807 | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, H. vulgare, Populus sp., T. aestivum

CPK-EF | Calcium-dependent protein kinase EF domain in the group Antibodies for Plant/Algal  / Developmental Biology / Signal transduction at Agrisera AB (Antibodies for research) (AS11 1807)

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Calcium-dependent protein kinase EF domain including Arabidopsis thalaiana TAIR: At4g35310 and At5g04870

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

60-65 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Hordeum vulgare, Populus sp., Triticum aestivum
Predicted reactivity Brassica napus, Nicotiana tabacum, Oryza sativa, Solanum lycopersicum, Solanum tuberosum
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

application example

western blot using anti-CPK-EF | Calcium-dependent protein kinase EF domain


20 µg of total protein from Hordeum vulgare extracted with isolation buffer with 6M urea were separated on 12% SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blots were blocked with 5% milk in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly once, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:25 000 in TBS-T containing2% milk for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 300 seconds.

Courtesy of Dr. Lucyna Misztal, Institute of Molecular Biology and Biotechnology, Poznań, Poland

Additional information

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Background

Background CDPKs are large group of kinases found/existing? only in plants and protists. They are involved in decoding Ca2+ signals in many stress conditions. CDPKs are composed of 4 domains: N-terminal variable domain, kinase domain, autoinhibitory domain and calmodulin-resembling domain (Calcium binding domain) - EF-hand domain.

Product citations

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