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Anti-CPX1 | Coproporphyrinogen-III oxidase 1
- Product Info
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Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana CPX1 sequence, UniProt:
UniProt: Q9LR75, TAIR: AT1G03475Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 50 µl, of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 2000 (WB)
Expected | apparent MW: 43.7 | 41 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana, Cucurbita pepo
Predicted reactivity: Brassica napus, Camelina sativa, Capsella rubella, Citrus sinensis, Eucalyptus grandis, Eutrema salsugineum, Jatropha curca, Manihot esculenta, Populus trichocarpa, Salix suchowensis, Tarenaya hassleriana, Quercus lobata Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Samples:
1: Cucurbita pepo sbsp pepo
2: Cucurbita pepo sbsp pepo
3: Cucurbita pepo sbsp pepo
4: Cucurbita pepo sbsp pepo
5: Arabidopsis thaliana Col-05 µg/well of total protein extracted freshly from Cucurbita pepo or Arabidopsis thaliana leaves were extracted with (0.2 M NaCl, 0.02 M Tris-HCl pH 7.5, 1 mM EDTA, 1 mM PMSF, 1% NP-40, 0.2% SDS and denatured with Laemmli sample buffer at 95°C for 4 min. Proteins were separated on 8% SDS-PAGE and blotted 1 h to PVDF (pore size of 45 µm), using semi-dry transfer. Blot was blocked with 5% milk for 1 h/RT. Blot was incubated in the primary antibody at a dilution of 1: 2 000 in TBS-T for ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602) diluted to 1: 5 000 in TBS-T for 1h/RT with agitation. The blot was washed as above and developed for 2 min with Agrisera ECLBright. Exposure time was 2 min.
Courtesy of Dr.Theoni Margaritopoulou, Benaki Phytopathological Institute, Greece
- Additional Information
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Additional information: - Background
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Background: CPX1 (Coproporphyrinogen-III oxidase 1) is a key enzyme of heme biosynthesis. It is catalyzing the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III.
Alternative name: Protein LESION INITIATION 2, AtCPO-I, Coprogen oxidase, Coproporphyrinogenase - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Posters Collection - Reviews:
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)