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CPX1 | Coproporphyrinogen-III oxidase 1

AS13 2753 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana

CPX1 | Coproporphyrinogen-III oxidase 1 in the group Antibodies Plant/Algal  / Photosynthesis  / Chlorophyll at Agrisera AB (Antibodies for research) (AS13 2753)
CPX1 | Coproporphyrinogen-III oxidase 1



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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana CPX1 sequence, UniProt:
UniProt: Q9LR75,  TAIR: AT1G03475

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl, of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution

1 : 2000 (WB)

Expected | apparent MW 43,7 | 41 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Cucurbita pepo
Predicted reactivity Brassica napus, Camelina sativa, Capsella rubella, Citrus sinensis, Eucalyptus grandis, Eutrema salsugineum, Jatropha curca, Manihot esculenta, Populus trichocarpa, Salix suchowensis, Tarenaya hassleriana, Quercus lobata
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples

 Western blot using anti-CPX1 (plant) antibodies

Samples:
1: Cucurbita pepo sbsp pepo
2: Cucurbita pepo sbsp pepo
3: Cucurbita pepo sbsp pepo
4: Cucurbita pepo sbsp pepo
5: Arabidopsis thaliana Col-0

5 µg/well of total protein extracted freshly from Cucurbita pepo or Arabidopsis thaliana leaves were extracted with (0.2M NaCl, 0.02M Tris-HCl pH 7.5, 1mM EDTA, 1mM PMSF, 1% NP-40, 0.2% SDS and denatured with Laemmli sample buffer at 95°C for 4 min. Proteins were separated on 8 % SDS-PAGE  and blotted 1h to PVDF (pore size of  45 um), using semi-dry transfer. Blot was blocked with 5 % milk for 1h/RT. Blot was incubated in the primary antibody at a dilution of 1: 2 000 in TBS-T for ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602) diluted to 1: 5 000 in TBS-T for 1h/RT with agitation. The blot was washed as above and developed for 2  min with Agrisera ECLBright. Exposure time was 2 min.

Courtesy of Dr.Theoni Margaritopoulou,Benaki Phytopathological Institute, Greece

Additional information

Additional information

 

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Background

Background CPX1 (Coproporphyrinogen-III oxidase 1) is a key enzyme of heme biosynthesis. It is catalyzing  the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III.
Alternative name: Protein LESION INITIATION 2, AtCPO-I, Coprogen oxidase, Coproporphyrinogenase

Product citations

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