Cytokinin | N6-isopentenyladenosine (1 mg)
AS09 415 | Clonality: Polyclonal | Host: Rabbit | Reactivity: higher plants
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Product Information
Immunogen
BSA-conjugated, via ribose, N6-isopentenyladenosine
Host
Rabbit
Clonality
Polyclonal
Purity
Total IgG. Protein G purified in PBS.
Format
Lyophilized
Quantity
1 mg
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
ELISA (ELISA), Radioimmunoassay (RIA)
Recommended dilution
2-10 µl/15 ml; Specific information about dilution is going to be included on the vial
Reactivity
Confirmed reactivity
N6-isopentenyladenosine
Predicted reactivity
N6-isopentenyladenosine
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Additional information
Additional information
Detection limit is 4,5 pg, 15 fmol, linear range of logit/log plot is 0,018-10 pmol, midrange (B/Bo =50 %) 135 pg, 149 fmol; for further antibody characteristics please check "more information" tab,These antibodies were made to N6-isopentenyladenosine and can bind free bases, ribosides, nucleotides and 9-glucosides
This antibody recognize a6A but not m6A or A, which iis caused by the geometrical similarity between isopentenyl and allyl (Shu et al., 2020).
Background
Background
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling.Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.
Product citations
Selected references
Shu et al. (2022) m6A-label-seq: A metabolic labeling protocol to detect transcriptome-wide mRNA N6-methyladenosine (m6A) at base resolution, STAR Protocols, Volume 3, Issue 1, 2022, 101096, ISSN 2666-1667, https://doi.org/10.1016/j.xpro.2021.101096.
Alvarez et al. (2020). Hormonal and gene dynamics in de novo shoot meristem formation during adventitious caulogenesis in cotyledons of Pinus pinea. Plant Cell Rep. 2020 Jan 28. doi: 10.1007/s00299-020-02508-0.
Alvarez et al. (2020). Hormonal and gene dynamics in de novo shoot meristem formation during adventitious caulogenesis in cotyledons of Pinus pinea. Plant Cell Rep. 2020 Jan 28. doi: 10.1007/s00299-020-02508-0.
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