Cytokinin | N6-isopentenyladenosine (1 mg)

AS09 415 | Clonality: Polyclonal | Host: Rabbit | Reactivity: higher plants

Cytokinin | N6-isopentenyladenosine (1 mg)

Product Information

Immunogen

BSA-conjugated, via ribose, N6-isopentenyladenosine

Host Rabbit

Clonality Polyclonal

Purity Total IgG. Protein G purified in PBS.

Format Lyophilized

Quantity 1 mg

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Radioimmunoassay (RIA)

Recommended dilution 2-10 µl/15 ml; Specific information about dilution is going to be included on the vial

Reactivity

Confirmed reactivity N6-isopentenyladenosine

Predicted reactivity N6-isopentenyladenosine

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Cytokinin | N6-isopentenyladenosine (1 mg)

This antibody recognize a6A but not m6A or A, which iis caused by the geometrical similarity between isopentenyl and allyl (Shu et al., 2020).

Background

Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling.Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.

Product citations

Selected references Shu et al. (2022) m6A-label-seq: A metabolic labeling protocol to detect transcriptome-wide mRNA N6-methyladenosine (m6A) at base resolution, STAR Protocols, Volume 3, Issue 1, 2022, 101096, ISSN 2666-1667, https://doi.org/10.1016/j.xpro.2021.101096.
Alvarez et al. (2020). Hormonal and gene dynamics in de novo shoot meristem formation during adventitious caulogenesis in cotyledons of Pinus pinea. Plant Cell Rep. 2020 Jan 28. doi: 10.1007/s00299-020-02508-0.

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Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	BSA-conjugated, via ribose, N6-isopentenyladenosine
Purity:	Total IgG. Protein G purified in PBS.
Quantity:	1 mg
recommended dilution:	2-10 µl/15 ml; Specific information about dilution is going to be included on the vial
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Radioimmunoassay (RIA)
background:	Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling.Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.
additional information:	Cytokinin \| N6-isopentenyladenosine (1 mg)
additional information (application):	This antibody recognize a6A but not m6A or A, which iis caused by the geometrical similarity between isopentenyl and allyl (Shu et al., 2020).
Confirmed reactivity:	N6-isopentenyladenosine
predicted reactivity:	N6-isopentenyladenosine
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
All references:	Shu et al. (2022) m6A-label-seq: A metabolic labeling protocol to detect transcriptome-wide mRNA N6-methyladenosine (m6A) at base resolution, STAR Protocols, Volume 3, Issue 1, 2022, 101096, ISSN 2666-1667, https://doi.org/10.1016/j.xpro.2021.101096. Alvarez et al. (2020). Hormonal and gene dynamics in de novo shoot meristem formation during adventitious caulogenesis in cotyledons of Pinus pinea. Plant Cell Rep. 2020 Jan 28. doi: 10.1007/s00299-020-02508-0.