Dendra2 tag
Product no: AS21 4623
AS21 4623 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Dendra2 tag
274
€
Delivery:
3-6 business days
- Product Info
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Immunogen: Dendra2 tag protein Host: Rabbit Clonality: Polyclonal Purity: Immunoglobulin purified on Protein A in PBS. Contains 15 mM sodium azide. Format: Liquid at 1 mg/ml. Quantity: 0,1 mg Storage: Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Immunocytochemistry (ICC), Flow cytometry (FlowCyt) (QC tested), Western blot (WB) - Reactivity
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Confirmed reactivity: Dendra2-tagged proteins - Background
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Background: Dendra2 is an improved version of green-to-red photo switchable protein Dendra, from an octocoral (Dendronephthya) and compared to it, Dendra2 exhibits brighter fluorescence before and after photoswitching. Excitation maximum of Dendra2 is 490 nm before and 553 nm after photoactivation, and its emission maximum is 507 nm before and 573 nm after photoactivation. Activating light for Dendra2 is UV/violet to blue. Nonactivated Dendra2 spectral characteristics are similar to EGFP, and this green fluorescence can be detected at low light intensities of blue light. At high intensities of the same blue light (or of UV/violet light) Dendra2 is photoactivated and gets emission characteristics similar to TRITC. - Protocols
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Agrisera Western Blot protocol and video tutorials
Antibody Protocols - Reviews:
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