Dendra2 tag

AS21 4623 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Dendra2 tag

Product Information

Immunogen Dendra2 tag protein

Host Rabbit

Clonality Polyclonal

Purity Immunoglobulin purified on Protein A in PBS. Contains 15 mM sodium azide.

Format Liquid at 1 mg/ml.

Quantity 0,1 mg

Storage Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunocytochemistry (ICC), Flow cytometry (FlowCyt) (QC tested), Western blot (WB)

Reactivity

Confirmed reactivity Dendra2-tagged proteins

Application examples

Additional information

Background

Background Dendra2 is an improved version of green-to-red photo switchable protein Dendra, from an octocoral (Dendronephthya) and compared to it, Dendra2 exhibits brighter fluorescence before and after photoswitching. Excitation maximum of Dendra2 is 490 nm before and 553 nm after photoactivation, and its emission maximum is 507 nm before and 573 nm after photoactivation. Activating light for Dendra2 is UV/violet to blue. Nonactivated Dendra2 spectral characteristics are similar to EGFP, and this green fluorescence can be detected at low light intensities of blue light. At high intensities of the same blue light (or of UV/violet light) Dendra2 is photoactivated and gets emission characteristics similar to TRITC.

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