showForm anti-DnaK2 | heat shock protein 70-2 (HSP70-2) antibody

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DnaK2 | Heat shock protein 70-2 (HSP70-2)

AS08 350  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Cyanobacteria

DnaK2 | Heat shock protein 70-2 (HSP70-2) in the group Antibodies for Plant/Algal  / Environmental Stress / Heat shock at Agrisera AB (Antibodies for research) (AS08 350)

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Product Information

Immunogen

full length recombinant DnaK2 protein derived from Synechocystis PCC 6803 DnaK2 protein sequence  UniProt: P22358

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

67.6 | 76 kDa

Reactivity

Confirmed reactivity Synechocystis PCC 6803
Predicted reactivity

Anabaena sp., Crocosphaera watsonii, Cyanothece sp. PCC 7424, Lyngbya sp. PCC 8106, Microcoleus chthonoplastes PCC 7420,  Nodularia spumigena CCY 9414, Nostoc sp.,  Synechococcus sp. PCC 7942 and other cyanobacterial strains

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example

10 µg of total protein from total cell extract of Synechocystis PCC 6803 wilde type (WT) and mutant strain that lacks Hsp16.6 were separated on  8 % gel and blotted on nitrocellulose membrane. Filters were blocked (1h), incubated with 1:1000 anti-DnaK2 antibodies (2h) followed by incubation with 1: 2500 secondary anti-rabbit (1h) coupled to HRP and visualization with chemiluminescent detection reagent, exposure time was 10 seconds.

Additional information

Additional information

This product can be sold containing proclin if requested

Related products

Related products

AS07 270 | Anti-DnaK, rabbit antibodies

Background

Background

DnaK2 belongs to the family of heat shock proteins 70 and functions as a chaperone involved in stress response. Dnak2 is heat induced but also present at high level under normal growth conditions.

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