DnaK2 | Heat shock protein 70-2 (HSP70-2)

Product no: AS08 350

AS08 350  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Cyanobacteria

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  • Product Info
  • Immunogen:

    full length recombinant DnaK2 protein derived from Synechocystis PCC 6803 DnaK2 protein sequence  UniProt: P22358

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 200 l
    Reconstitution: For reconstitution add 200 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 67,6 | 76 kDa
  • Reactivity
  • Confirmed reactivity: Synechocystis PCC 6803
    Predicted reactivity:

    Anabaena sp., Crocosphaera watsonii, Cyanothece sp. PCC 7424, Lyngbya sp. PCC 8106, Microcoleus chthonoplastes PCC 7420,  Nodularia spumigena CCY 9414, Nostoc sp.,  Synechococcus sp. PCC 7942 and other cyanobacterial strains


    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example

    10 µg of total protein from total cell extract of Synechocystis PCC 6803 wilde type (WT) and mutant strain that lacks Hsp16.6 were separated on  8 % gel and blotted on nitrocellulose membrane. Filters were blocked (1h), incubated with 1:1000 anti-DnaK2 antibodies (2h) followed by incubation with 1: 2500 secondary anti-rabbit (1h) coupled to HRP and visualization with chemiluminescent detection reagent, exposure time was 10 seconds.

  • Additional Information
  • Additional information:

    This product can be sold containing proclin if requested

  • Background
  • Background:

    DnaK2 belongs to the family of heat shock proteins 70 and functions as a chaperone involved in stress response. Dnak2 is heat induced but also present at high level under normal growth conditions.

  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts


    Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

    Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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Accessories

AS07 270  |  Clonality: Polyclonal  |  Host: Rabbit   |  Reactivity: Chlamydomonas reinhardtii, Synechocystis 6803 motile, Synechocystis 6803 GT (glucose tolerant strain), Synechococcus elongates 7942

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AS09 607 |  Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)

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