Name:
Phone:
E-mail:
Message
Tag Antibody Set 

DnaK | chloroplast stromal chaperone

AS07 270  |  Clonality: Polyclonal  |  Host: Rabbit   |  Reactivity: Chlamydomonas reinhardtii, Synechocystis 6803 motile, Synechocystis 6803 GT (glucose tolerant strain), Synechococcus elongates 7942

DnaK | chloroplast stromal chaperone in the group Antibodies for Plant/Algal  / Photosynthesis  / Chaperones/Transporters at Agrisera AB (Antibodies for research) (AS07 270)

PRODUCT INFORMATION IN PDF

Qty: 
272 €
Buy 2 items of this product for 202 €/items
Buy 3 items of this product for 185 €/items

Datasheet Product citations Protocols Add review

Product Information

Immunogen

recombiant DNAK of Chlamydomonas reinhardtii

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW

70 kDa

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii, Synechocystis 6803 motile, Synechocystis 6803 GT (glucose tolerant strain), Synechococcus elongates sp. PCC7942
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example

western blot using anti-Chlamydomonas DnaK antibodies


15 µg of Arabidopsis thaliana leaf extract (1), 10 µg of total protein from: Synechocystis 6803 motile (2), Synechocystis 6803 GT (glucose tolerant strain) (3), Synechococcus elongates 7942 (4), Marker - P ierce™ Prestained Protein MW Marker (kat #26612): Total protein was extracted with following buffer: 10 mM Tris HC l, pH 8.0, 0.5% LDS, 4% glycerol, 0.1 mM EDTA were mixed with sample buffer and denatured for 5 min at 95°C. Samples were separated on 10% S DS -PAGE a nd b lo tted 1 h to nitrocellulose membrane (Amersha m Protran) using tank wet transfer (Bio -Rad) in standard transfer buffer in presence of 20% methanol. Transfer of proteins to the membrane was checked using 0,5% Ponceau S staining before the blocking step. Blots were blocked in buffer (2 % lo w -fat milk in 1xPBS, 0,1% Tween) for 1 h at room temperature (RT) with agitation. Blots were incubated in the primary antibody at a dilution of 1 : 5000 for 1 h at RT with agitation. The antibody solutionwas decanted and the blot was rinsed briefly twice, then washed once f or 15 min and 3 times for 5 min in PBS -T at RT with agitation. Blot was incubated in secondary antibody (goat anti-rabbit IgG, AS09 602, Agrisera ) dilut ed to 1 :30 000 in for 1 h at RT with agitation. The blot was was washed as above and developed for 5 min with Clarity Western ECL Substrate and ChemiDoc detection system (Bio-Rad).

Courtesy Dr. Elena Pojidaeva, Laboratory of Plant Gene Expression, Timiryazev Institute of Plant Physiology RAS, 127276 Moscow Russia

Additional information

It is not determined which isoform of DnaK is recognized by this antibody in Arabidopsis thaliana.

Related products

Related products

AS07 271 | Anti-DnaJ | prokaryotic heat shock protein, rabbit antibodies

collection of antibodies to heat shock proteins

collection of antibodies to chloroplastic proteins

Background

Background

Procaryotic Hsp70 protein family includes DnaK, HscA (Hsc66), HscC (Hsc62). Those proteins are involved in protein folding, cell protection from environmental stress and other functions which are vital for a living cell.

Product citations

Selected references Göhre et al. (2006). One of Two Alb3 Proteins Is Essential for the Assembly of the Photosystems and for Cell Survival in Chlamydomonas The Plant Cell 18:1454–1466.

Related products: DnaK | chloroplast stromal chaperone

AS16 ECL-SN-10 | low pico to mid femtogram and extreme low femtogram detection
15 €
AS07 271  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: ...
355 €
AS09 607 Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)
186 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
181 €