showForm Donkey anti-goat IgG (H&L), ALP (alkaline phosphatase) conjugat

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Donkey anti-Goat IgG (H&L), ALP conjugated, min. cross-reactivity to human, mouse, rabbit, rat IgG

AS10 1043 | Clonality: Polyclonal  |  Host: Donkey  |  Reactivity: Goat IgG (H&L)

https://www.agrisera.com/en/artiklar/animal-cell-biology/secondary-antibodies-2/anti-Goat-igg-secondary-antibodies-2/unconjugated-2/index.html
Donkey anti-Goat IgG (H&L), ALP conjugated, min. cross-reactivity to human, mouse, rabbit, rat IgG  in the group Secondary Antibodies / Anti-Goat / ALP (alkaline phosphatase) at Agrisera AB (Antibodies for research) (AS10 1043)

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Product Information

Host Donkey
Clonality Polyclonal
Purity Affinity purified IgG
Format Liquid
Quantity 0.5 mg
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Recommended dilution 1 : 50-1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC), 1 : 500-1 : 2000 (WB)

Reactivity

Application examples

Additional information

Additional information

This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins or IgG from human, mouse, rabbit, or rat based on immunoelectrophoresis.

Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

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Background

Background Donkey anti-goat IgG (H&L) is an ALP (alkaline phosphatase) conjugated secondary antibody which reacts with all goat IgGs in immunological assays. Antibody is adsorbed to human,mouse,rabbit,rat IgG and affinity purified using solid phase goat IgG.

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