Donkey anti-Goat IgG (H&L), ALP conjugated, min, cross-reactivity to human, mouse, rabbit, rat IgG

Product no: AS10 1043

AS10 1043 | Clonality: Polyclonal  |  Host: Donkey  |  Reactivity: Goat IgG (H&L)

https://www.agrisera.com/en/artiklar/animal-cell-biology/secondary-antibodies-2/anti-Goat-igg-secondary-antibodies-2/unconjugated-2/index.html
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Delivery:  3-6 business days
  • Product Info
  • Host: Donkey
    Clonality: Polyclonal
    Purity: Immunogen affinity purified IgG.
    Format: Liquid
    Quantity: 0,5 mg
    Storage: Non-diluted antibody is stable for 4 years at 2-8C. For storage at -20C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
    Recommended dilution: 1 : 50-1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC), 1 : 500-1 : 2000 (WB)
  • Additional Information
  • Additional information:

    This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.

    Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins or IgG from human, mouse, rabbit, or rat based on immunoelectrophoresis.

    Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.

    BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

  • Background
  • Background: Donkey anti-goat IgG (H&L) is an ALP (alkaline phosphatase) conjugated secondary antibody which reacts with all goat IgGs in immunological assays. Antibody is adsorbed to human,mouse,rabbit,rat IgG and affinity purified using solid phase goat IgG.
  • Product Citations
  • Selected references: 38221900
  • Protocols
  • Antibody protocols
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