Donkey anti-Goat IgG (H&L), DyLight® 594 conjugated

Name: Donkey anti-Goat IgG (H&L), DyLight® 594 conjugated
SKU: AS12 1997
Price: 206 EUR
Availability: InStock

AS12 1997 | Clonality: Polyclonal | Host: Donkey | Reactivity: Goat IgG (H&L)

Donkey anti-Goat IgG (H&L), DyLight® 594 conjugated in the group Secondary Antibodies / Anti-Goat / Fluorescent / DyLight® / DyLight® 594 at Agrisera AB (Antibodies for research) (AS12 1997)

Product Information

Immunogen

Purified goat IgG, whole molecule

Host Donkey

Clonality Polyclonal

Purity Immunogen affinity purified donkey IgG.

Format Lyophilized

Quantity 1 mg

Reconstitution For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Recommended dilution 1 : 20-1 : 2000 for most applications

Reactivity

Application examples

Additional information

Additional information Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative

Based on immunoelectrophoresis, this antibody reacts with: heavy chains on goat IgG light chains on all goat immunoglobulins.

No reactivity is observed to: non-immunoglobulin goat serum proteins.

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

Background

Donkey anti-Goat IgG (H&L) , DyLight®594 Conjugate is a secondary antibody conjugated to DyLight® 594, which binds to all goat IgGs in immunological assays.

DyLight® 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase goat IgG.

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Product citations

Selected references Ainla et al. (2013). Lab on a Biomembrane: rapid prototyping and manipulation of 2D fluidic lipid bilayers circuits. Sci Rep. 2013 Sep 25;3:2743. doi: 10.1038/srep02743.

immunogen:	Purified goat IgG, whole molecule
Reconstitution:	For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Host:	Donkey
Clonality:	Polyclonal
Purity:	Immunogen affinity purified donkey IgG.
Format:	Lyophilized
Quantity:	1 mg
storage:	Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
recommended dilution:	1 : 20-1 : 2000 for most applications
additional information:	Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
additional information (application):	Based on immunoelectrophoresis, this antibody reacts with: heavy chains on goat IgG light chains on all goat immunoglobulins. No reactivity is observed to: non-immunoglobulin goat serum proteins. BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
background:	Donkey anti-Goat IgG (H&L) , DyLight®594 Conjugate is a secondary antibody conjugated to DyLight® 594, which binds to all goat IgGs in immunological assays. DyLight® 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase goat IgG. DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
All references:	Ainla et al. (2013). Lab on a Biomembrane: rapid prototyping and manipulation of 2D fluidic lipid bilayers circuits. Sci Rep. 2013 Sep 25;3:2743. doi: 10.1038/srep02743.

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