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Donkey anti-Goat IgG (H&L), DyLightŪ 594 conjugated

AS12 1997 | Clonality: Polyclonal  |  Host: Donkey  | Reactivity: Goat IgG (H&L)

Donkey anti-Goat IgG (H&L), DyLightŪ 594 conjugated in the group Secondary Antibodies / Anti-Goat / Fluorescent / DyLightŪ / DyLightŪ 594 at Agrisera AB (Antibodies for research) (AS12 1997)



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Product Information

Immunogen

Purified goat IgG, whole molecule

Host Donkey
Clonality Polyclonal
Purity Immunogen affinity purified donkey IgG.
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Recommended dilution 1 : 20-1 : 2000 for most applications

Reactivity

Application examples

Additional information

Additional information Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative

Based on  immunoelectrophoresis, this antibody reacts with:   heavy chains on goat IgG   light chains on all goat immunoglobulins.

No reactivity is observed to: non-immunoglobulin goat serum proteins.

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

Related products

Background

Background

Donkey anti-Goat IgG (H&L) , DyLight®594 Conjugate is a secondary antibody conjugated to  DyLight® 594, which binds to all goat IgGs in immunological assays. 

DyLight® 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase goat IgG.

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Product citations

Selected references Ainla et al. (2013). Lab on a Biomembrane: rapid prototyping and manipulation of 2D fluidic lipid bilayers circuits. Sci Rep. 2013 Sep 25;3:2743. doi: 10.1038/srep02743.
immunogen:

Purified goat IgG, whole molecule

Reconstitution: For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Host: Donkey
Clonality: Polyclonal
Purity: Immunogen affinity purified donkey IgG.
Format: Lyophilized
Quantity: 1 mg
storage: Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
recommended dilution: 1 : 20-1 : 2000 for most applications
additional information: Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
additional information (application):

Based on  immunoelectrophoresis, this antibody reacts with:   heavy chains on goat IgG   light chains on all goat immunoglobulins.

No reactivity is observed to: non-immunoglobulin goat serum proteins.

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

background:

Donkey anti-Goat IgG (H&L) , DyLight®594 Conjugate is a secondary antibody conjugated to  DyLight® 594, which binds to all goat IgGs in immunological assays. 

DyLight® 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase goat IgG.

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

All references: Ainla et al. (2013). Lab on a Biomembrane: rapid prototyping and manipulation of 2D fluidic lipid bilayers circuits. Sci Rep. 2013 Sep 25;3:2743. doi: 10.1038/srep02743.

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