Donkey anti-Goat IgG (H&L), DyLight® 650 conjugated

AS12 2253 | Clonality: Polyclonal | Host: Donkey | Reactivity: Goat IgG (H&L)

Product Information

Immunogen

purified goat IgG (H&L), whole molecule

Host Donkey

Clonality Polyclonal

Purity Immunogen affinity purified donkey IgG.

Format Lyophilized

Quantity 1 mg

Reconstitution For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Recommended dilution 1 : 20-1 : 2000 for most applications

Reactivity

Not reactive in

Application examples

Additional information

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

Based on immunoelectrophoresis, this antibody reacts with: heavy chains on goat IgG light chains on all goat immunoglobulins

No reactivity is observed to: non-immunoglobulin goat serum proteins.

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

Background

Donkey anti-Goat IgG (H&L) - DyLight®650 Conjugated is a secondary antibody conjugated to DyLight® 650, which binds to Goat IgG (H&L) in immunological assays.

DyLight® 650 has Amax = 652 nm, Emax = 672 nm. Antibodies are are affinity purified using solid phase goat IgG (H&L).

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Product citations

Picture (footer):
not reactive in:
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Donkey
immunogen:	purified goat IgG (H&L), whole molecule
Purity:	Immunogen affinity purified donkey IgG.
Quantity:	1 mg
recommended dilution:	1 : 20-1 : 2000 for most applications
Reconstitution:	For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
storage:	Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
background:	Donkey anti-Goat IgG (H&L) - DyLight®650 Conjugated is a secondary antibody conjugated to DyLight® 650, which binds to Goat IgG (H&L) in immunological assays. DyLight® 650 has Amax = 652 nm, Emax = 672 nm. Antibodies are are affinity purified using solid phase goat IgG (H&L). DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
additional information:	Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative. Based on immunoelectrophoresis, this antibody reacts with: heavy chains on goat IgG light chains on all goat immunoglobulins No reactivity is observed to: non-immunoglobulin goat serum proteins. BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
additional information (application):

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