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Donkey anti-Rabbit IgG (H&L), DyLightŪ 550 conjugated, min. cross-reactivity to human IgG and serum proteins

AS12 1961 | Clonality: Polyclonal  |  Host: Donkey | Reactivity: Rabbit  IgG

Donkey anti-Rabbit IgG (H&L), DyLightŪ 550 conjugated, min. cross-reactivity to human IgG and serum proteins in the group Secondary Antibodies / Anti-Rabbit / Fluorescent / DyLightŪ / DyLightŪ 550 at Agrisera AB (Antibodies for research) (AS12 1961)

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Product Information

Immunogen

Purified rabbit IgG, whole molecule

Host Donkey
Clonality Polyclonal
Purity Affinity purified donkey IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Recommended dilution 1 : 20-1 : 2000 for most applications

Reactivity

Application examples

Additional information

Additional information

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins.

No reactivity is observed to: non-immunoglobulin rabbit serum proteins mouse IgG or serum proteins.

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Background

Background

Donkey anti-Rabbit IgG (H&L) - DyLight®550 Conjugated, min. cross-reactivity to human IgG and serum proteins is a secondary antibody conjugated to  DyLight® 550, which binds to all rabbit IgG (H&L) in immunological assays. 

DyLight® 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase rabbit IgG (H&L).

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Product citations

Related products: Donkey anti-Rabbit IgG (H&L), DyLightŪ 550 conjugated, min. cross-reactivity to human IgG and serum proteins