Donkey anti-Rabbit IgG (H&L), ALP conjugated

Product no: AS10 841

AS10 841 | Clonality: Polyclonal  |  Host: Donkey  | Reactivity: Rabbit IgG (heavy and light chains - H&L)

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  • Product Info
  • Immunogen:

    Purified Rabbit IgG

    Host: Donkey
    Clonality: Polyclonal
    Purity: Immunogen affinity purified donkey IgG.
    Format: Liquid
    Quantity: 1 mg
    Storage: Non-diluted antibody is stable for 4 years at 2-8C. For storage at -20C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
    Tested applications: ELISA (ELISA) , Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
    Recommended dilution: 1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC),, 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
  • Reactivity
  • Confirmed reactivity: Rabbit IgG (H&L)
    Predicted reactivity: Rabbit IgG (H&L)
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information: ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
    Additional information (application):

    This antibody reacts with alpha (heavy chains) on rabbit IgG and all light chains on rabbit immunoglobulins based on immunoelectrophoresis.

    No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis.

  • Background
  • Background:

    Donkey anti-rabbit IgG (H&L) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to rabbit IgG  (H&L) in immunological assays.

  • Product Citations
  • Selected references: Kay et al. (2014). Elevations in Th2-initiating cytokines (IL-33, IL-25, TSLP) in lesional skin from Chronic Spontaneous ("Idiopathic") Urticaria. Br J Dermatol. 2014 Dec 18. doi: 10.1111/bjd.13621.
  • Protocols
  • Antibody protocols
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