Donkey anti-Rabbit IgG (H&L), FITC conjugated

AS10 1299 | Clonality: Polyclonal | Host:Donkey | Reactivity: Rabbit IgG (H&L)

Product Information

Host Donkey

Clonality Polyclonal

Purity Immunogen affinity purified IgG.

Format Liquid

Quantity 2 mg

Storage Store at 2-8°C. Shelf life of this product is one year from date of receipt.

Recommended dilution 1 : 20-1 : 2000 (IHC), 1 : 50-1 : 5000 (ICC) (Flow cyt)

Reactivity

Application examples

Additional information

This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.

Background

Background Donkey anti-rabbit IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated is a secondary antibody which binds to rabbit IgG in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase rabbit IgG.

Product citations

Selected references Kozieł et al. (2017). Subcelullar localization of proteins associated with Prune dwarf virus replication. Eur. J. Pathology DOI: 10.1007/s10658-017-1215-8.

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