Donkey anti-Rabbit IgG (H&L), FITC conjugated
AS10 1299 | Clonality: Polyclonal | Host:Donkey | Reactivity: Rabbit IgG (H&L)
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Product Information
Host
Donkey
Clonality
Polyclonal
Purity
Immunogen affinity purified IgG.
Format
Liquid
Quantity
2 mg
Storage
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
Recommended dilution
1 : 20-1 : 2000 (IHC), 1 : 50-1 : 5000 (ICC) (Flow cyt)
Reactivity
Application examples
Additional information
Additional information
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.
Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Background
Background
Donkey anti-rabbit IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated is a secondary antibody which binds to rabbit IgG in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase rabbit IgG.
Product citations
Selected references
Kozieł et al. (2017). Subcelullar localization of proteins associated with Prune dwarf virus replication. Eur. J. Pathology DOI: 10.1007/s10658-017-1215-8.
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