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Donkey anti-Rabbit IgG (H&L), F(ab)'2 Fragment, DyLightŪ 488 conjugated

AS16 3526 | Clonality: Polyclonal | Host: Donkey | Reactivity: Rabbit IgG (H&L), F(ab)'2 Fragment
Donkey anti-Rabbit IgG (H&L), F(ab)'2 Fragment, DyLightŪ 488 conjugated in the group Secondary Antibodies / Anti-Rabbit / Fluorescent / DyLightŪ / DyLightŪ 488 at Agrisera AB (Antibodies for research) (AS16 3526)

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Product Information

Immunogen Purified Rabbit IgG, whole molecule
Host Donkey
Clonality Polyclonal
Purity Affinity purified
Format Lyophilized
Quantity 0.5 mg
Reconstitution For reconstitution add 0.55 ml of sterile water.Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store freeze-dried powder at 2-8 °C.
Tested applications Flow cytometry (Flow cyt), Immunolocalization (IL)
Recommended dilution 1 : 20-1 : 2000 (Flow cyt), (IL)

Reactivity

Confirmed reactivity Rabbit IgG (H&L)

Application examples

Additional information

Additional information

Antibody purity is > 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Affinity purified using solid phase Rabbit IgG .
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.

Based on IEP, this antibody reacts with: • heavy (γ) chains on rabbit IgG • light chains on all rabbit immunoglobulins .
Based on IEP, no reactivity is observed to: • non-immunoglobulin rabbit serum proteins .

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Background

Background

Donkey anti-Rabbit IgG (H&L), F(ab)'2 Fragment, DyLight® 488 conjugated is a secondary antibody conjugated to  DyLight® 488, which binds to rabbit IgG in immunological assays. 
DyLight® 488 has Amax = 493 nm, Emax = 518 nm.

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Product citations

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