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Donkey anti-Rat IgG (H&L), DyLight® 800 conjugated

AS12 2468 | Clonality: Polyclonal | Host: Donkey | Reactivity: Rat IgG (H&L)

Donkey anti-Rat IgG (H&L), DyLight® 800 conjugated in the group Human/Animal Antibodies / Secondary Antibodies/Blocking / Anti-rat / Fluorescent / DyLight® / DyLight® 800 at Agrisera AB (Antibodies for research) (AS12 2468)

PRODUCT INFORMATION IN PDF

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product information
Background		Donkey anti-Rat IgG (H&L), DyLight®800 Conjugated is a secondary antibody conjugated to DyLight® 800, which binds to Rat IgG (H&L) in immunological assays. DyLight® 800 has Amax = 777 nm, Emax = 794 nm. Antibodies are purified using solid phase Rat IgG (H&L) DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
Immunogen		Purified Rat IgG, whole molecule
Host		Donkey
Clonality		Polyclonal
Clone
Purity		Affinity purified donkey IgG
Format		Lyophilized
Quantity		1 mg
Reconstitution		For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage		Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications
Related products		other DyLight® anti-Rat secondary antibodies
Additional information		Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative. Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG, light chains on all rat immunoglobulins No reactivity is observed to: non-immunoglobulin rat serum proteins

application information
Recommended dilution		1 : 20-1 : 2000 for most applications
Expected \| apparent MW
Confirmed reactivity
Predicted reactivity
Not reactive in
Additional information
Selected references		to be added when available

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