Donkey anti-Rat IgG (H&L), HRP conjugated

Product no: AS10 947

AS10 947 |Clonality: Polyclonal  | Host: Donkey | Reactivity: Rat IgG (H&L)

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  • Data sheet
  • Donkey anti-Rat IgG (H&L), HRP conjugated
  • Product Info
  • Host: Donkey
    Clonality: Polyclonal
    Purity: Immunogen affinity purified donkey IgG.
    Format: Lyophilized
    Quantity: 1 mg
    Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily
    Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
    Recommended dilution: The optimal working dilution should be determined by the investigator
  • Additional Information
  • Additional information:

    This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.

    Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins by immunoelectrophoresis.

    Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.

  • Background
  • Background: Donkey anti-rat IgG (H&L), is a secondary HRP (horse radish peroxidase) conjugated antibody which binds to rat IgGs in immunological assays.
  • Product Citations
  • Selected references: Bui et al. (2020). Differential submergence tolerance between juvenile and adult Arabidopsis plants involves the ANAC017 transcription factor. doi.org/10.1101/2020.02.12.945923 BioRxiv.
  • Protocols
  • Antibody protocols
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