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Donkey anti-Rat IgG (H&L), HRP conjugated

AS10 947 |Clonality: Polyclonal  | Host: Donkey | Reactivity: Rat IgG (H&L)

Donkey anti-Rat IgG (H&L), HRP conjugated in the group Secondary Antibodies / Anti-Rat / HRP (horse radish peroxidase) at Agrisera AB (Antibodies for research) (AS10 947)

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170
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Product Information

Host Donkey
Clonality Polyclonal
Purity Affinity purified donkey IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Recommended dilution The optimal working dilution should be determined by the investigator.

Reactivity

Application examples

Additional information

Additional information

This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins by immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.

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Background

Background Donkey anti-rat IgG (H&L), is a secondary HRP (horse radish peroxidase) conjugated antibody which binds to rat IgGs in immunological assays.

Product citations

Selected references Bui et al. (2020). Differential submergence tolerance between juvenile and adult Arabidopsis plants involves the ANAC017 transcription factor. doi.org/10.1101/2020.02.12.945923 BioRxiv.

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