Donkey anti-Sheep IgG (H&L), Biotin conjugated

Product no: AS09 611

AS09 611 | Clonality: Polyclonal  |  Host: Donkey  | Reactivity: Sheep IgG (H&L)

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Delivery:  3-6 business days
  • Product Info
  • Immunogen:

    Purified sheep IgG, whole molecule

    Host: Donkey
    Clonality: Polyclonal
    Purity: Immunogen purified donkey IgG.
    Format: Liquid
    Quantity: 2 mg
    Storage: Store non-diluted antibody at 2-8C. Shelf life is 1 year from date of receipt.
    Tested applications: ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
    Recommended dilution: 1 : 2000-1: 20 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 2000-1: 20 000 (WB)
  • Reactivity
  • Confirmed reactivity: Sheep IgG heavy and light chains (H&L)
    Predicted reactivity: Sheep IgG Heavy and Light chains (H&L)
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information:

    Antibody concentration is 3.85 mg/ml and it has been afinity purified on solid phase sheep IgG (H&L)

    biotin conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2,  1 % (w/v) BSA, Protease/IgG free. It contains 0.05 % sodium azide as preservative

    Additional information (application):

    No reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis.

    BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

  • Background
  • Background:

    Donkey anti-sheep IgG is a secondary antibody conjugated to biotin which binds to sheep IgG immunoglobulins in immunological assays.

  • Product Citations
  • Selected references: 38221900
  • Protocols
  • Agrisera Western Blot Guide
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