Donkey anti-Sheep IgG (H&L), Biotin conjugated

AS09 611 | Clonality: Polyclonal | Host: Donkey | Reactivity: Sheep IgG (H&L)

Product Information

Immunogen

Purified sheep IgG, whole molecule

Host Donkey

Clonality Polyclonal

Purity Immunogen purified donkey IgG.

Format Liquid

Quantity 2 mg

Storage Store non-diluted antibody at 2-8°C. Shelf life is 1 year from date of receipt.

Tested applications ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 2000-1: 20 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 2000-1: 20 000 (WB)

Reactivity

Confirmed reactivity Sheep IgG heavy and light chains (H&L)

Predicted reactivity Sheep IgG Heavy and Light chains (H&L)

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Antibody concentration is 3.85 mg/ml and it has been afinity purified on solid phase sheep IgG (H&L)

biotin conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. It contains 0.05 % sodium azide as preservative

No reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis.

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

Background

Donkey anti-sheep IgG is a secondary antibody conjugated to biotin which binds to sheep IgG immunoglobulins in immunological assays.

Product citations

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additional information:	Antibody concentration is 3.85 mg/ml and it has been afinity purified on solid phase sheep IgG (H&L) biotin conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. It contains 0.05 % sodium azide as preservative
additional information (application):	No reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis. BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
background:	Donkey anti-sheep IgG is a secondary antibody conjugated to biotin which binds to sheep IgG immunoglobulins in immunological assays.
Confirmed reactivity:	Sheep IgG heavy and light chains (H&L)
predicted reactivity:	Sheep IgG Heavy and Light chains (H&L)
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Clonality:	Polyclonal
Format:	Liquid
Host:	Donkey
immunogen:	Purified sheep IgG, whole molecule
Purity:	Immunogen purified donkey IgG.
Quantity:	2 mg
recommended dilution:	1 : 2000-1: 20 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 2000-1: 20 000 (WB)
storage:	Store non-diluted antibody at 2-8°C. Shelf life is 1 year from date of receipt.
tested applications:	ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)