Donkey anti-Sheep IgG (H&L), FITC conjugated, min, cross-reactivity to human, mouse,rabbit IgG

Product no: AS10 1024

AS10 1024 | Clonality: Polyclonal  |  Host: Donkey |  Reactivity: Sheep IgG (H&L)

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  • Product Info
  • Host: Donkey
    Clonality: Polyclonal
    Purity: Immunogen affinity purified IgG.
    Format: Liquid
    Quantity: 1 mg
    Storage: Store at 2-8C. Shelf life of this product is one year from date of receipt.
    Recommended dilution: 1 : 20-1 : 2000 (IHC), 1 : 50-1 : 5000 (ICC) (Flow cyt)
  • Additional Information
  • Additional information:

    This antibody reacts with the heavy chains on sheep IgG and with the light chains on all sheep immunoglobulins based on immunoelectrophoresis.

    Minimum cross-reactivity is observed to non-immunoglobulin sheep serum proteins and human,mouse,rabbit IgG based on immunoelectrophoresis.

    Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 1% (w/v) BSA, Protease/IgG free and 0.05 % (w/v) sodium azide as preservative.

    BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

  • Background
  • Background: Donkey anti-sheep IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to all sheep IgGs in immunological assays.  FITC has Amax = 494 nm, Emax = 518 nm.Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibody is adsorbed against  human,mouse,rabbit IgG and affinity purified using solid phase sheep IgG.
  • Protocols
  • Antibody protocols
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