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Donkey anti-Sheep IgG (H&L), TRITC conjugated, min. cross-reactivity to human, rabbit IgG

AS10 1226   | Clonality: Polyclonal  |  Host:Donkey   |  Reactivity:Sheep IgG (H&L)

Donkey anti-Sheep IgG (H&L), TRITC conjugated, min. cross-reactivity to human, rabbit IgG  in the group Secondary Antibodies / Anti-Sheep / Fluorescent / FITC, TRITC at Agrisera AB (Antibodies for research) (AS10 1226)

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Product Information

Host Donkey
Clonality Polyclonal
Purity Affinity purified IgG
Format Liquid
Quantity 2 mg
Storage Store at 2-8°C.Shelf life of this product is one year from date of receipt.
Recommended dilution The optimal working dilution should be determined by the investigator.

Reactivity

Application examples

Additional information

Additional information

This antibody reacts with the heavy chains on sheep IgG and with the light chains on all sheep immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin sheep serum proteins and human,rabbit IgG based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 1% (w/v) BSA, Protease/IgG free and 0.05 % (w/v) sodium azide as preservative.

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

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Background

Background

Donkey anti-sheep IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated, is a secondary antibody with binds to sheep IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are adsorbed against human,rabbit IgG and are affinity purified using solid phase sheep IgG.

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