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Donkey anti-Sheep IgG (H&L), TRITC conjugated, min, cross-reactivity to human, rabbit IgG
AS10 1226 | Clonality: Polyclonal | Host: Donkey | Reactivity: Sheep IgG (H&L)
- Product Info
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Host: Donkey Clonality: Polyclonal Purity: Immunogen affinity purified IgG. Format: Lyophilized Quantity: 1 mg Reconstitution: For reconstitution, add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Spin centrifuge shortly to remove any particles. Prepare fresh working dilutions daily. Storage: Store at 2-8°C. Shelf life of this product is one year from date of receipt. Recommended dilution: The optimal working dilution should be determined by the investigator, - Additional Information
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Additional information: This antibody reacts with the heavy chains on sheep IgG and with the light chains on all sheep immunoglobulins based on immunoelectrophoresis.
Minimum cross-reactivity is observed to non-immunoglobulin sheep serum proteins and human,rabbit IgG based on immunoelectrophoresis.
Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 1% (w/v) BSA, Protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG. - Background
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Background: Donkey anti-sheep IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated, is a secondary antibody with binds to sheep IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are adsorbed against human,rabbit IgG and are affinity purified using solid phase sheep IgG.
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- Antibody protocols
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