eEF1b | Elongation factor 1-beta

Name: eEF1b | Elongation factor 1-beta
SKU: AS07 265
Price: 302 EUR
Availability: InStock

AS07 265 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, Z. mays

eEF1b | Elongation factor 1-beta in the group Antibodies Plant/Algal / DNA/RNA/Cell Cycle / Translation at Agrisera AB (Antibodies for research) (AS07 265)

Product Information

Immunogen

Recombinant eEF1B-beta protein from Arabidopsis thaliana with no affinity tag, UniProt: Q9SI20,TAIR: At2g18110

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 200 µl

Reconstitution For reconstitution add 200 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW

25 | 30 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Zea mays

Predicted reactivity Brassica campestris, Hordeum vulgare, Nicotiana attenuata, Oryza sativa Pisum sativum, Ricinus communis
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application example

western blot using anti-eEF1b antibodies

10 µg of total cell extract from Arabidopsis thaliana (1), 5 µg of total cell extract from Arabidopsis thaliana (2), 2 ng of recombinant eEF1b (3), 4 ng of recombinant eEF1b (4), 8 ng of recombinant eEF1b (5) were separated on 15% SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 1000 for 1h at room temperature with agitation and secondary HRP-conjugated antibody (1: 10 000).

Additional information

Background

eEF1B-beta protein belongs to a family of elongation factors, proteins which are involved in translational elongation. eEF1B-beta protein is localized in plant cytosol. Alternative names: EF-1-delta 2, elongation factor 1B-beta 2

Product citations

Selected references Lázaro-Mixteco et al. (2012). The Absence of Heat Shock Protein HSP101 Affects the Proteome of Mature and Germinating Maize Embryos. J. Proteome Res. May 11, ahead of print.

background:	eEF1B-beta protein belongs to a family of elongation factors, proteins which are involved in translational elongation. eEF1B-beta protein is localized in plant cytosol. Alternative names: EF-1-delta 2, elongation factor 1B-beta 2
calculated \| apparent molecular mass [kDa]:	25 \| 30 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	Recombinant eEF1B-beta protein from Arabidopsis thaliana with no affinity tag, UniProt: Q9SI20,TAIR: At2g18110
Purity:	Serum
Quantity:	200 µl
recommended dilution:	1 : 1000 (WB)
Reconstitution:	For reconstitution add 200 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
Picture (footer):	Application example 10 µg of total cell extract from Arabidopsis thaliana (1), 5 µg of total cell extract from Arabidopsis thaliana (2), 2 ng of recombinant eEF1b (3), 4 ng of recombinant eEF1b (4), 8 ng of recombinant eEF1b (5) were separated on 15% SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 1000 for 1h at room temperature with agitation and secondary HRP-conjugated antibody (1: 10 000).
All references:	Lázaro-Mixteco et al. (2012). The Absence of Heat Shock Protein HSP101 Affects the Proteome of Mature and Germinating Maize Embryos. J. Proteome Res. May 11, ahead of print.
Confirmed reactivity:	Arabidopsis thaliana, Zea mays
predicted reactivity:	Brassica campestris, Hordeum vulgare, Nicotiana attenuata, Oryza sativa Pisum sativum, Ricinus communis Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known