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eEF1b | Elongation factor 1-beta
AS07 265 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, Z. mays
- Product Info
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Immunogen: Recombinant eEF1B-beta protein from Arabidopsis thaliana with no affinity tag, UniProt: Q9SI20,TAIR: At2g18110
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 200 µl Reconstitution: For reconstitution add 200 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 25 | 30 kDa
- Reactivity
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Confirmed reactivity: Arabidopsis thaliana, Zea mays
Predicted reactivity: Brassica campestris, Hordeum vulgare, Nicotiana attenuata, Oryza sativa Pisum sativum, Ricinus communis
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Application example
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10 µg of total cell extract from Arabidopsis thaliana (1), 5 µg of total cell extract from Arabidopsis thaliana (2), 2 ng of recombinant eEF1b (3), 4 ng of recombinant eEF1b (4), 8 ng of recombinant eEF1b (5) were separated on 15% SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 1000 for 1h at room temperature with agitation and secondary HRP-conjugated antibody (1: 10 000).
- Background
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Background: eEF1B-beta protein belongs to a family of elongation factors, proteins which are involved in translational elongation. eEF1B-beta protein is localized in plant cytosol. Alternative names: EF-1-delta 2, elongation factor 1B-beta 2
- Product Citations
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Selected references: Lázaro-Mixteco et al. (2012). The Absence of Heat Shock Protein HSP101 Affects the Proteome of Mature and Germinating Maize Embryos. J. Proteome Res. May 11, ahead of print. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts - Reviews:
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