eEF1b | Elongation factor 1-beta

AS07 265 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, Z. mays

eEF1b | Elongation factor 1-beta in the group Antibodies Plant/Algal  / DNA/RNA/Cell Cycle / Translation at Agrisera AB (Antibodies for research) (AS07 265)
eEF1b | Elongation factor 1-beta


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Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information


Recombinant eEF1B-beta protein from Arabidopsis thaliana with no affinity tag, UniProt: Q9SI20,TAIR: At2g18110

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

25 | 30 kDa


Confirmed reactivity Arabidopsis thaliana, Zea mays
Predicted reactivity Brassica campestris, Hordeum vulgare, Oryza sativa Pisum sativum, Ricinus communis
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples

Application example

western blot using anti-eEF1b antibodies


10 µg of total cell extract from Arabidopsis thaliana (1), 5 µg of total cell extract from Arabidopsis thaliana (2), 2 ng of recombinant eEF1b (3), 4 ng of recombinant eEF1b (4), 8 ng of recombinant eEF1b (5) were separated on 15% SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 1000 for 1h at room temperature with agitation and secondary HRP-conjugated antibody (1: 10 000).

Additional information

Related products

Related products

AS11 1633 | Anti-EF1A | elongation factor 1-alpha / EF-1-alpha, rabbit antibodies

AS10 679 | Anti-eEF1B-alpha2 | elongation factor 1B-alpha 2, rabbit antibodies

AS10 678 | Anti-eEF1B-alpha1 an 2 | elongation factor 1B-alpha 1 and 2, rabbit antibodies

AS10 677 | Anti-eEF1B-beta1 and 2 | elongation factor 1-beta1 and 1-beta2, rabbit antibodies

AS10 676 | Anti-eEF1B-gamma1 and 2 | elongation factor 1-gamma 1 and 2, rabbit antibodies

collection of antibodies to DNA/RNA metabolism



eEF1B-beta protein belongs to a family of elongation factors, proteins which are involved in translational elongation. eEF1B-beta protein is localized in plant cytosol. Alternative names: EF-1-delta 2, elongation factor 1B-beta 2

Product citations

Selected references Lázaro-Mixteco et al. (2012). The Absence of Heat Shock Protein HSP101 Affects the Proteome of Mature and Germinating Maize Embryos. J. Proteome Res. May 11, ahead of print.

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