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EF1A | Elongation factor 1-alpha / EF-1-alpha

AS11 1633   | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity:Arabidopsis thaliana, Triticum aestivum

EF1A | Elongation factor 1-alpha / EF-1-alpha in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / Translation at Agrisera AB (Antibodies for research) (AS11 1633)

DATA SHEET IN PDF

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373
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Data sheet Product citations Protocols Add review

Product Information

Immunogen

Full length, recombinant EF1A of Arabidopsis thaliana UniProt: P0DH99-1, TAIR: AT1G07940

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

49.5 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Oryza sativa, Triticum aestivum
Predicted reactivity Brassica napus, Picea sitchensis, Populus trichocarpa, Solanum tuberosum, Sorghum bicolor, Zea mays
Species of your interest not listed? Contact us
Not reactive in Recombinant wheat EF1A

Application examples

Application examples

application example

western blot detection of plant EF1A
10 µg total protein extracted from 15 day old Arabidopsis thaliana seedlings that were either kept at 22°C (3) or heat treated at 38°C for 2 hours  prior to protein extraction (4). As positive control 10 ng of recombinant AtEF1A (2) were separated side by side with the plant samples on 10-15 % gradient SDS-PAGE and blotted to nitrocellulose (Bio-rad). Blots were blocked following transfer with 5% low fat milk in low salt buffer for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 1000 for O/N 4°C with agitation in the blocking solution. The primary antibody solution was removed and the blot was rinsed briefly twice, then washed 4 times for 15 min each at room temperature with agitation using low salt buffer. Blots were incubated in secondary antibody, anti-rabbit IgG horse radish peroxides conjugated, from Agrisera, AS09 602, diluted to 1:35 000 for 1h at room temperature with agitation then washed as above and treated with ECL detection reagent according to the manufacturer's instructions.  Exposure time was 5 seconds.

Lane (1) with no signal: recombinant Triticum spp. EF1A.

Courtesy Dr. Eman Basha, Arizona State University

Additional information

Related products

Related products

AS10 679 | Anti-eEF1B-alpha2 | elongation factor 1B-alpha 2, rabbit antibodies

AS10 678 | Anti-eEF1B-alpha1 an 2 | elongation factor 1B-alpha 1 and 2, rabbit antibodies

AS10 677 | Anti-eEF1B-beta1 and 2 | elongation factor 1-beta1 and 1-beta2, rabbit antibodies

AS07 265 | Anti-eEF1b | elongation factor eEF1b-beta protein, rabbit antibodies

AS10 676 | Anti-eEF1B-gamma1 and 2 | elongation factor 1-gamma 1 and 2, rabbit antibodies

Plant protein extraction buffer

Background

Background

EF1A (elongation factor 1-alpha) belongs to the GTP-binding elongation factor family and is localized in the cytoplasm. It is an essential enzyme in elongation phase of protein synthesis. There are four genes encoding EF1A in Arabidopsis, sequences of genes 1,2 and 3 are identical. Synonymes:eEF-1A.

Product citations

Selected references Djukić et al. (2019). Resolving subcellular plant metabolism. Plant J. 2019 Jul 30. doi: 10.1111/tpj.14472.
Zhen et al. (2018). 2D-DIGE comparative proteomic analysis of developing wheat grains under high-nitrogen fertilization revealed key differentially accumulated proteins that promote storage protein and starch biosyntheses. Anal Bioanal Chem. 2018 Jul 30. doi: 10.1007/s00216-018-1230-4.
Wang et al. (2016). GOLGI TRANSPORT 1B Regulates Protein Export from the Endoplasmic Reticulum in Rice Endosperm Cells. Plant Cell. 2016 Nov;28(11):2850-2865. (Oryza sativa, western blot)

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