Name:
Phone:
E-mail:
Address:






eEF1B-beta1 and 2 | elongation factor 1-beta1 and 1-beta2

AS10 677 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

eEF1B-beta1 and 2 | elongation factor 1-beta1 and 1-beta2 in the group Antibodies Plant/Algal  / DNA/RNA/Cell Cycle / Translation at Agrisera AB (Antibodies for research) (AS10 677)
eEF1B-beta1 and 2 | elongation factor 1-beta1 and 1-beta2



DATA SHEET IN PDF

Qty: 
394
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen

recombinant eEF1B-beta1 protein from Arabidopsis thaliana with no affinity tag, P48006, At1g30230

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 2000 (WB)
Expected | apparent MW

25 | 39 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Oryza sativa, Ricinus communis, Solanum tuberosum, Zea mays, Vitis vinifera
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples

Application example
western blot using anti elongation factor beta 1 and beta 2antibodies

10 µg total protein extracted from 15 day old Arabidopsis thaliana seedlings that were either kept at 22ºC (1) or heat treated at 38ºC for 2 hours prior to protein extraction (2). As positive control 10 ng of recombinant elongation factor proteins beta 1 (3) and beta 2 (4) were separated side by side with the plant samples on 11% SDS-PAGE and blotted to nitrocellulose (Bio-rad). Blots were blocked following transfer with 5% low fat milk in low salt buffer for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 2000 for 2h at room temperature with agitation in the blocking solution. The primary antibody solution was removed and the blot was rinsed briefly twice, then washed 4 times for 15 min each at room temperature with agitation using low salt buffer. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxides conjugated), diluted to 1:2500 for 1h at room temperature with agitation then washed as above and treated with ECL detection reagent according to the manufacturers instructions. Exposure time was 5 seconds. The primary antibody could be reused if it is kept at 4ºC for 2 weeks and if frozen at -20ºC for long time.

Low salt buffer components are 10 mM Tris (pH 7.6), 68 mM NaCl and 0.05 % Triton X-100.

Additional information

Additional information

Antibody is recognizing both proteins: elonagtion factor beta 1 and beta 2

Related products

Background

Background

eEF1B-beta1 protein belongs to a family of elongation factors, proteins which are involved in translational elongation. Alternative names: Elongation factor 1-delta 1, EF-1-delta 1

Product citations

Selected references McLoughlin et al. (2019) HSP101 Interacts with the Proteasome and Promotes the Clearance of Ubiquitylated Protein Aggregates. Plant Physiol. 2019 Aug;180(4):1829-1847. doi: 10.1104/pp.19.00263
McLoughlin et al. (2016) Class I and II Small Heat Shock Proteins Together with HSP101 Protect Protein Translation Factors during Heat Stress. Plant Physiol. 2016 Oct;172(2):1221-1236.
Picture (footer):

Application example
western blot using anti elongation factor beta 1 and beta 2antibodies

10 µg total protein extracted from 15 day old Arabidopsis thaliana seedlings that were either kept at 22ºC (1) or heat treated at 38ºC for 2 hours prior to protein extraction (2). As positive control 10 ng of recombinant elongation factor proteins beta 1 (3) and beta 2 (4) were separated side by side with the plant samples on 11% SDS-PAGE and blotted to nitrocellulose (Bio-rad). Blots were blocked following transfer with 5% low fat milk in low salt buffer for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 2000 for 2h at room temperature with agitation in the blocking solution. The primary antibody solution was removed and the blot was rinsed briefly twice, then washed 4 times for 15 min each at room temperature with agitation using low salt buffer. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxides conjugated), diluted to 1:2500 for 1h at room temperature with agitation then washed as above and treated with ECL detection reagent according to the manufacturers instructions. Exposure time was 5 seconds. The primary antibody could be reused if it is kept at 4ºC for 2 weeks and if frozen at -20ºC for long time.

Low salt buffer components are 10 mM Tris (pH 7.6), 68 mM NaCl and 0.05 % Triton X-100.

Confirmed reactivity: Arabidopsis thaliana
predicted reactivity: Oryza sativa, Ricinus communis, Solanum tuberosum, Zea mays, Vitis vinifera
Species of your interest not listed? Contact us
not reactive in: No confirmed exceptions from predicted reactivity are currently known
calculated | apparent molecular mass [kDa]:

25 | 39 kDa

Clonality: Polyclonal
Format: Lyophilized
Host: Rabbit
immunogen:

recombinant eEF1B-beta1 protein from Arabidopsis thaliana with no affinity tag, P48006, At1g30230

Purity: Serum
Quantity: 200 ĩl
recommended dilution: 1 : 2000 (WB)
Reconstitution: For reconstitution add 200 ĩl of sterile water
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: Western blot (WB)
All references: McLoughlin et al. (2019) HSP101 Interacts with the Proteasome and Promotes the Clearance of Ubiquitylated Protein Aggregates. Plant Physiol. 2019 Aug;180(4):1829-1847. doi: 10.1104/pp.19.00263
McLoughlin et al. (2016) Class I and II Small Heat Shock Proteins Together with HSP101 Protect Protein Translation Factors during Heat Stress. Plant Physiol. 2016 Oct;172(2):1221-1236.
background:

eEF1B-beta1 protein belongs to a family of elongation factors, proteins which are involved in translational elongation. Alternative names: Elongation factor 1-delta 1, EF-1-delta 1

additional information:

Antibody is recognizing both proteins: elonagtion factor beta 1 and beta 2

Related products: eEF1B-beta1 and 2 | elongation factor 1-beta1 and 1-beta2

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...
From 26 €
AS10 676 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana
394 €
AS09 607 |  Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)

10 % until...
206 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 ...
201 €