eEF1B-gamma1 and 2 | elongation factor 1-gamma 1 and 2
AS10 676 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana
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Product Information
Recombinant eEF1B-gamma1 protein from Arabidopsis thaliana with no affinity tag,UniProt: O04487, TAIR: At1g09640
46 kDa
Reactivity
Application examples
Application example
10 µg total protein extracted from 15 day old Arabidopsis thaliana seedlings that were either kept at 22ºC (1) or heat treated at 38ºC for 2 hours prior to protein extraction (2). As positive control 10 ng of recombinant elongation factor proteins gamma 1 (3) and gamma 2 (4) were separated side by side with the plant samples on 11% SDS-PAGE and blotted to nitrocellulose (Bio-rad). Blots were blocked following transfer with 5% low fat milk in low salt buffer for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 2000 for 2h at room temperature with agitation in the blocking solution. The primary antibody solution was removed and the blot was rinsed briefly twice, then washed 4 times for 15 min each at room temperature with agitation using low salt buffer. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxides conjugated), diluted to 1:2500 for 1h at room temperature with agitation then washed as above and treated with ECL detection reagent according to the manufacturers instructions. Exposure time was 5 seconds.
Low salt buffer components are 10 mM Tris (pH 7.6), 68 mM NaCl and 0.05 % Triton X-100.
Additional information
Background
eEF1B-gamma1 protein belongs to a family of elongation factors, proteins which are involved in translational elongation. Alternative names: EF-1-gamma 1, eEF-1B gamma 1
Product citations
McLoughlin et al. (2016) Class I and II Small Heat Shock Proteins Together with HSP101 Protect Protein Translation Factors during Heat Stress. Plant Physiol. 2016 Oct;172(2):1221-1236.
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