IKP

GST-tag

Product no: AS20 4513

AS20 4513 | Clonality: Polyclonal | Host: Rabbit | Reactivity: GST-tag

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  • Product Info
  • Immunogen: Recombinant, full size GST, amino acids 1-212, NCBI: AAA57089
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum. Contains 0.05 % sodium azide.
    Format: Liquid
    Quantity: 100 l
    Storage: Store at -20C, Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Immunoprecipitation (IP), Western blot (WB)
    Recommended dilution: assay dependent (ELISA), (IP), 1 : 2000 - 1: 10 000 (WB)
  • Reactivity
  • Confirmed reactivity: GST-tag
  • Application Examples
  • Immunoprecipiation and Western blot with anti-GST tag antibodies

    -: Lysate of 293T cells transfected with an empty vector +: Lysate of 293T cells transfected with the plasmid carrying the GST-tagged INbox gene
    Transfer: nitrocellulose memrbane. Primary antibodies: 1: 1000 1h/RT in TBS-T. Secondary antibodies HRP conjugated. Detection using chemiluminescence, following manufacture's recommendations. 
  • Background
  • Background: GST-tag (glutathione S-transferase) is a tag added to N-terminus of a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG tags.
  • Product Citations
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  • Protocols
  • Antibody protocols
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