AS20 4513 | Clonality: Polyclonal | Host: Rabbit | Reactivity: GST-tag

GST-tag in the group Alla produktegenskaper at Agrisera AB (Antibodies for research) (AS20 4513)

Data sheet Protocols Add review
immunogen: Recombinant, full size GST, amino acids 1-212, NCBI: AAA57089
Host: Rabbit
Clonality: Polyclonal
Purity: Serum. Contains 0.05 % sodium azide.
Format: Liquid
Quantity: 100 ĩl
storage: Store at -20°C, Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications: ELISA (ELISA), Immunoprecipitation (IP), Western blot (WB)
recommended dilution: assay dependent (ELISA), (IP), 1 : 2000 - 1: 10 000 (WB)
Confirmed reactivity: GST-tag
Picture (footer): Immunoprecipiation and Western blot with anti-GST tag antibodies

-: Lysate of 293T cells transfected with an empty vector +: Lysate of 293T cells transfected with the plasmid carrying the GST-tagged INbox gene
Transfer: nitrocellulose memrbane. Primary antibodies: 1: 1000 1h/RT in TBS-T. Secondary antibodies HRP conjugated. Detection using chemiluminescence, following manufacture's recommendations. 
background: GST-tag (glutathione S-transferase) is a tag added to N-terminus of a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG tags.
All references:

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