eIF2-alpha | Eukaryotic translation initiation factor 2 subunit alpha

Product no: AS20 4371

AS20 4371   | Clonality: Polyclonal |  Host: Rabbit  | Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana eIF2-alpha, UniProt: Q9FE78, TAIR: AT5G05470
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 g
    Reconstitution: For reconstitution add 50 µl, of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 38,8 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Brassica napus, Brassica oleracea, Camelina sativa, Capsella rubella, Medicago truncatula, Noccaea caerulescens, Papaver somniferum, Raphanus sativus, Tarenaya hassleriana

    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Western blot using anti-eIF2 antibodies
    Samples:
    1-4 14 days old Arabidopsis thaliana wild-type
    5 - Arabidopsis thaliana gcn2 mutant

    Arabidopsis thaliana whole seedlings were grinded in liquid nitrogen and 80 mg of tissue powder was resuspended in 100 µl of 0.5xSDS Laemmli buffer in a 1.5 ml tube. After 5 minutes of mixing at room temperature, tubes were centrifuged at 21 000 xg for 10 minutes at RT. For immunoblog analysis 10 µl of supernatant was separated on a 12.5 % (w/v) SDS-PAGE gel and electroblotted onto PVDF membrane. After 1 h of blocking at RT with 1x blocking buffer (TBS-T, 5 % non-fat milk and 0.2 % BSA), the membrane was washed with 1xTBS-T four times for 10 minutes each. Post washing, membrane was incubated with anti-eIF2alpha antibodies at 1: 5000 for 48 h at 4°C. Following the washing with TBS-T, 10 minutes each for six repeats, the membrane was incubated in the goat anti-rabbit IgG, HRP conjugated secondary antibodies for 1h at RT followed by washing with TBS-T 10 minutes each for ten repeats and the blot was developed with chemiluminescent detection reagents, according to manufacture's recommendations. 

    Courtesy of Dr. Ansul Lokdarshi, Biochemistry & Cellular and Molecular Biology, University of Tennessee, USA
  • Background
  • Background: eIF2-alpha (Eukaryotic translation initiation factor 2 subunit alpha)  functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. eIF2 is a heterodimer composed of an alpha, beta and gamma chain.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Poster Collection
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Buy 2 items of this product for 226.00 €/items
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