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eIF1 | Eukaryotic initiation factor eIF1

AS19 4253  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana, Lactuca sativa, Nicotiana tabacum, Pisum sativum, Triticum aestivum

eIF1 | Eukaryotic initiation factor eIF1 in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / Translation at Agrisera AB (Antibodies for research) (AS19 4253)

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Data sheet Product citations Protocols Add review

Product Information

Immunogen

Recombinant eIF1 of Triticum aestivum

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 8000 (WB)
Expected | apparent MW

49 | 50 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Lactuca sativa, Nicotiana tabacum, Pisum sativum, Triticum aestivum
Predicted reactivity higher plants
Species of your interest not listed? Contact us

Application examples

Application examples

Application example

Western blot using anti-eIF1 antibodies

Recommend incubation for 1 hr at room temperature in 5% non-fat dry milk in phosphate-buffer saline with 0.5% Tween-80 (PBST). Figure shows plant leaf extracts or wheat germ extract (5 microL of the supernatant of 50 mg liquid nitrogen powdered tissue/100 microL 2X SDS Laemmli buffer containing protease inhibitor crushed with a plastic pestle in a microfuge tube and centrifuged for 10 min at 4 C.) Image shown is using Agrisera Anti-rabbit HRP (1/30,000) for 1 hr at RT in 5% non-fat dry milk in phosphate-buffer saline with 0.5% Tween-80 (PBST) and using Agrisera Ultra Bright substrate and exposed for 5 seconds in a BioRad ChemDoc on high resolution. Blue star indicates eIF1 band.

Courtesy Dr. Karen Browning, The University of Texas at Austin , USA

Additional information

Related products

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AS10 676 | Anti-eEF1B-gamma1 and 2 | elongation factor 1-gamma 1 and 2, rabbit antibodies
Plant protein extraction buffer

Secondary antibodies

Background

Background

eIF1 (Eukaryotic initiation factor eIF1) is involved in protein biosynthesis. 

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