eIF1 | Eukaryotic initiation factor eIF1

Name: eIF1 | Eukaryotic initiation factor eIF1
SKU: AS19 4253
Price: 302 EUR
Availability: InStock

AS19 4253 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Lactuca sativa, Nicotiana tabacum, Pisum sativum, Triticum aestivum

eIF1 | Eukaryotic initiation factor eIF1 in the group Antibodies Plant/Algal / DNA/RNA/Cell Cycle / Translation at Agrisera AB (Antibodies for research) (AS19 4253)

eIF1 | Eukaryotic initiation factor eIF1

Product Information

Immunogen

Recombinant eIF1 of Triticum aestivum

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 200 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 8000 (WB)

Expected | apparent MW

49 | 50 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Lactuca sativa, Nicotiana tabacum, Pisum sativum, Triticum aestivum

Predicted reactivity higher plants
Species of your interest not listed? Contact us

Application examples

Application example

Western blot using anti-eIF1 antibodies

Recommend incubation for 1 hr at room temperature in 5% non-fat dry milk in phosphate-buffer saline with 0.5% Tween-80 (PBST). Figure shows plant leaf extracts or wheat germ extract (5 microL of the supernatant of 50 mg liquid nitrogen powdered tissue/100 microL 2X SDS Laemmli buffer containing protease inhibitor crushed with a plastic pestle in a microfuge tube and centrifuged for 10 min at 4 C.) Image shown is using Agrisera Anti-rabbit HRP (1/30,000) for 1 hr at RT in 5% non-fat dry milk in phosphate-buffer saline with 0.5% Tween-80 (PBST) and using Agrisera Ultra Bright substrate and exposed for 5 seconds in a BioRad ChemDoc on high resolution. Blue star indicates eIF1 band.

Courtesy Dr. Karen Browning, The University of Texas at Austin , USA

Additional information

Background

eIF1 (Eukaryotic initiation factor eIF1) is involved in protein biosynthesis.

Product citations

Related products: eIF1 | Eukaryotic initiation factor eIF1

eIF4A | Eukaryotic initiation factor eIF4A

AS19 4251 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabi...

302 €

Buy 2 items of this product for 226.00 €/items

Buy 3 items of this product for 205.00 €/items

eIF5 | Eukaryotic initiation factor eIF5

AS19 4252 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabi...

302 €

Buy 2 items of this product for 226.00 €/items

Buy 3 items of this product for 205.00 €/items

Goat anti-Rabbit IgG (H&L), ALP conjugated

AS09 607 | Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)

10 % until...

206 €

Goat anti-Rabbit IgG (H&L), HRP conjugated

AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 ...

201 €

immunogen:	Recombinant eIF1 of Triticum aestivum
Reconstitution:	For reconstitution add 200 µl of sterile water
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	50 µl
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
recommended dilution:	1 : 8000 (WB)
calculated \| apparent molecular mass [kDa]:	49 \| 50 kDa
Confirmed reactivity:	Arabidopsis thaliana, Lactuca sativa, Nicotiana tabacum, Pisum sativum, Triticum aestivum
predicted reactivity:	higher plants Species of your interest not listed? Contact us
Picture (footer):	Application example Recommend incubation for 1 hr at room temperature in 5% non-fat dry milk in phosphate-buffer saline with 0.5% Tween-80 (PBST). Figure shows plant leaf extracts or wheat germ extract (5 microL of the supernatant of 50 mg liquid nitrogen powdered tissue/100 microL 2X SDS Laemmli buffer containing protease inhibitor crushed with a plastic pestle in a microfuge tube and centrifuged for 10 min at 4 C.) Image shown is using Agrisera Anti-rabbit HRP (1/30,000) for 1 hr at RT in 5% non-fat dry milk in phosphate-buffer saline with 0.5% Tween-80 (PBST) and using Agrisera Ultra Bright substrate and exposed for 5 seconds in a BioRad ChemDoc on high resolution. Blue star indicates eIF1 band. Courtesy Dr. Karen Browning, The University of Texas at Austin , USA
background:	eIF1 (Eukaryotic initiation factor eIF1) is involved in protein biosynthesis.