eIF4A | Eukaryotic initiation factor eIF4A

AS19 4251 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Lactuca sativa, Nicotiana tabacum,Triticum aestivum, Pisum sativum

Product Information

Immunogen

Recombinant eIF4A of Triticum aestivum

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution 1: 10 000 - 1 : 20 000 (WB)

Expected | apparent MW

47.2 | 50 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Lactuca sativa, Nicotiana tabacum,Triticum aestivum, Pisum sativum

Predicted reactivity Aegilops tauschii, Actinidia chinensis var. chinensis, Albuca bracteata, Ananas comosus, Brachypodium distachyon, Carex duriuscula subsp. rigescens, Dichanthelium oligosanthes, Dorcoceras hygrometricum, Eleusine indica, Nicotiana benthamiana, Nicotiana sylvestris, Oryza sativa subsp. japonica, Panicum miliaceum, Pennisetum americanum, Phoenix dactylifera, Setaria italica, Sorghum bicolor, Trema orientale, Triticum aestivum, Zea mays
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Application examples

Application examples Application example

Recommend incubation for 1 hr at room temperature in 5% non-fat dry milk in phosphate-buffer saline with 0.5% Tween-80 (PBST). Figure shows plant leaf extracts or wheat germ extract (5 microL of the supernatant of 50 mg liquid nitrogen powdered tissue/100 microL 2X SDS Laemmli buffer containing protease inhibitor crushed with a plastic pestle in a microfuge tube and centrifuged for 10 min at 4 C.). Image shown is using Agrisera Anti-rabbit HRP (1/30,000) for 1 hr at RT in 5% non-fat dry milk in phosphate-buffer saline with 0.5% Tween-80 (PBST) and using Agrisera Ultra Bright substrate and exposed for 2 seconds in a BioRad ChemDoc on high resolution. Blue star indicates eIF4A band.

Courtesy Dr. Karen Browning, The University of Texas at Austin , USA