eIF4A | Eukaryotic initiation factor eIF4A

AS19 4251 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Lactuca sativa, Nicotiana tabacum,Triticum aestivum, Pisum sativum

eIF4A | Eukaryotic initiation factor eIF4A

Product Information

Immunogen

Recombinant eIF4A of Triticum aestivum

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1: 10 000 - 1 : 20 000 (WB)

Expected | apparent MW 47,2 | 50 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Lactuca sativa, Nicotiana benthamiana, Nicotiana tabacum,Triticum aestivum, Pisum sativum

Predicted reactivity Aegilops tauschii, Actinidia chinensis var. chinensis, Albuca bracteata, Ananas comosus, Brachypodium distachyon, Carex duriuscula subsp. rigescens, Dichanthelium oligosanthes, Dorcoceras hygrometricum, Eleusine indica, Nicotiana benthamiana, Nicotiana sylvestris, Oryza sativa subsp. japonica, Panicum miliaceum, Pennisetum americanum, Phoenix dactylifera, Setaria italica, Sorghum bicolor, Trema orientale, Triticum aestivum, Zea mays
Species of your interest not listed? Contact us

Application examples

Application examples Application example

Recommend incubation for 1 hr at room temperature in 5% non-fat dry milk in phosphate-buffer saline with 0.5% Tween-80 (PBST). Figure shows plant leaf extracts or wheat germ extract (5 microL of the supernatant of 50 mg liquid nitrogen powdered tissue/100 microL 2X SDS Laemmli buffer containing protease inhibitor crushed with a plastic pestle in a microfuge tube and centrifuged for 10 min at 4 C.). Image shown is using Agrisera Anti-rabbit HRP (1/30,000) for 1 hr at RT in 5% non-fat dry milk in phosphate-buffer saline with 0.5% Tween-80 (PBST) and using Agrisera Ultra Bright substrate and exposed for 2 seconds in a BioRad ChemDoc on high resolution. Blue star indicates eIF4A band.

Courtesy Dr. Karen Browning, The University of Texas at Austin , USA

Additional information

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Plant protein extraction buffer

Secondary antibodies

Background

eIF4A (Eukaryotic initiation factor eIF4A) is involved in protein biosynthesis.

Product citations

Selected references Li et al. (2021) Efficient and high-throughput pseudorecombinant-chimeric Cucumber mosaic virus-based VIGS in maize. Plant Physiol. 2021 Dec 4;187(4):2865-2876. doi: 10.1093/plphys/kiab443. PMID: 34606612; PMCID: PMC8644855.