eIF4A | Eukaryotic initiation factor eIF4A

Product no: AS19 4251
AS19 4251  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana, Lactuca sativa, Nicotiana tabacum,Triticum aestivum, Pisum sativum
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  • Product Info
  • Immunogen:

    Recombinant eIF4A of Triticum aestivum

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 l
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1: 10 000 - 1 : 20 000 (WB)
    Expected | apparent MW: 47,2 | 50 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Lactuca sativa, Nicotiana benthamiana, Nicotiana tabacum,Triticum aestivum, Pisum sativum
    Predicted reactivity: Aegilops tauschii, Actinidia chinensis var. chinensis, Albuca bracteata, Ananas comosus, Brachypodium distachyon, Carex duriuscula subsp. rigescens, Dichanthelium oligosanthes, Dorcoceras hygrometricum, Eleusine indica, Nicotiana benthamiana, Nicotiana sylvestris, Oryza sativa subsp. japonica, Panicum miliaceum, Pennisetum americanum, Phoenix dactylifera, Setaria italica, Sorghum bicolor, Trema orientale, Triticum aestivum, Zea mays
    Species of your interest not listed? Contact us
  • Application Examples
  • Application example


    western blot using anti-eIF4A antibodies


    Recommend incubation for 1 hr at room temperature in 5% non-fat dry milk in phosphate-buffer saline with 0.5% Tween-80 (PBST). Figure shows plant leaf extracts or wheat germ extract (5 microL of the supernatant of 50 mg liquid nitrogen powdered tissue/100 microL 2X SDS Laemmli buffer containing protease inhibitor crushed with a plastic pestle in a microfuge tube and centrifuged for 10 min at 4 C.). Image shown is using Agrisera Anti-rabbit HRP (1/30,000) for 1 hr at RT in 5% non-fat dry milk in phosphate-buffer saline with 0.5% Tween-80 (PBST) and using Agrisera Ultra Bright substrate and exposed for 2 seconds in a BioRad ChemDoc on high resolution. Blue star indicates eIF4A band.

    Courtesy Dr. Karen Browning, The University of Texas at Austin , USA
  • Background
  • Background:

    eIF4A (Eukaryotic initiation factor eIF4A) is involved in protein biosynthesis. 

  • Product Citations
  • Selected references: Li et al. (2021) Efficient and high-throughput pseudorecombinant-chimeric Cucumber mosaic virus-based VIGS in maize. Plant Physiol. 2021 Dec 4;187(4):2865-2876. doi: 10.1093/plphys/kiab443. PMID: 34606612; PMCID: PMC8644855.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts


    Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

    Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items
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