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  eIF4A | Eukaryotic initiation factor eIF4A
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      <strong>AS19 4251</strong>  | Clonality: <strong>Polyclonal </strong> |  Host: <strong>Rabbit </strong> |  Reactivity: <strong><em>Arabidopsis thaliana, <span class="st">Lactuca sativa</span>, Nicotiana tabacum,Triticum aestivum, Pisum sativum</em></strong>  
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      <h3>Product Information</h3>
          
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          <span>Immunogen</span>
          <span><p>Recombinant <span id="admin_page_title"><span class="rubrik_namn_liten">eIF4A of <em>Triticum aestivum</em></span></span></p></span>
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          <span>Host</span>
          <span>Rabbit</span>
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          <span>Clonality</span>
          <span>Polyclonal</span>
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          <span>Purity</span>
          <span>Serum</span>
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          <span>Format</span>
          <span>Lyophilized</span>
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          <span>Quantity</span>
          <span>50 µl</span>
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          <span>Reconstitution</span>
          <span>For reconstitution add 50 µl of sterile water.</span>
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          <span>Storage</span>
          <span>Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.</span>
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          <span>Tested applications</span>
          <span>Western blot (WB)</span>
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          <span>Recommended dilution</span>
          <span>1: 10 000 - 1 : 20 000 (WB)</span>
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          <span>Expected | apparent MW</span>
          <span><p>47.2 | 50 kDa</p></span>
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      <h3>Reactivity</h3>
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          <span>Confirmed reactivity</span>
          <span><em>Arabidopsis thaliana, <span class=st>Lactuca sativa</span>, Nicotiana tabacum,Triticum aestivum, Pisum sativum<br /></em></span>
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          <span>Predicted reactivity</span>
          <span><em>Aegilops tauschii, Actinidia chinensis var. chinensis, Albuca bracteata, Ananas comosus, Brachypodium distachyon, Carex duriuscula subsp. rigescens, Dichanthelium oligosanthes, Dorcoceras hygrometricum, Eleusine indica, Nicotiana benthamiana, Nicotiana sylvestris, Oryza sativa subsp. japonica, Panicum miliaceum, Pennisetum americanum, Phoenix dactylifera, Setaria italica, Sorghum bicolor, Trema orientale, Triticum aestivum, Zea mays</em><br /> Species of your interest not listed?  <a href=https://www.agrisera.com/en/info/contact-us.html target=_blank rel=noopener noreferrer>Contact us</a></span>
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              <span>Application examples</span>
              <span><strong>Application example<br /><br /><br /><img src="https://www.agrisera.com/dokument/bibliotek/Image/AS19 4251.jpg" alt="western blot using anti-eIF4A antibodies" width="350" height="450" /></strong> <br /><br />Recommend incubation for 1 hr at room temperature in 5% non-fat dry milk in phosphate-buffer saline with 0.5% Tween-80 (PBST). Figure shows plant leaf extracts or wheat germ extract (5 microL of the supernatant of 50 mg liquid nitrogen powdered tissue/100 microL 2X SDS Laemmli buffer containing protease inhibitor crushed with a plastic pestle in a microfuge tube and centrifuged for 10 min at 4 C.). Image shown is using Agrisera Anti-rabbit HRP (1/30,000) for 1 hr at RT in 5% non-fat dry milk in phosphate-buffer saline with 0.5% Tween-80 (PBST) and using Agrisera Ultra Bright substrate and exposed for 2 seconds in a BioRad ChemDoc on high resolution. Blue star indicates eIF4A band. <br /><br />Courtesy Dr. Karen Browning, The University of Texas at Austin , USA</span>
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<p><a href="en/artiklar/eef1b-alpha1-elongation-factor-1b-alpha-1.html" target="_blank" rel="noopener noreferrer">AS10 678</a> | Anti-eEF1B-alpha1 an 2 | elongation factor 1B-alpha 1 and 2, rabbit antibodies</p>
<p><a href="https://www.agrisera.com/en/artiklar/plant_algal-cell-biology/dna_rna_cell-cycle/eef1b-beta1-elongation-factor-1-beta1.html" target="_blank" rel="noopener noreferrer">AS10 677</a> | Anti-eEF1B-beta1 and 2 | elongation factor 1-beta1 and 1-beta2, rabbit antibodies</p>
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<p><a href="https://www.agrisera.com/en/artiklar/plant_algal-cell-biology/secondary-antibodies/anti-rabbit-igg-scondary-antibodies/index.html" target="_blank" rel="noopener noreferrer">Secondary antibodies</a></p></span>
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       <h3>Background</h3>
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              <span>Background</span>
              <span><p><span id="admin_page_title"><span class="rubrik_namn_liten"><strong>eIF4A (Eukaryotic initiation factor eIF4A)</strong> is involved in protein biosynthesis. </span></span></p></span>
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