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eIF5 | Eukaryotic initiation factor eIF5

AS19 4252  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana, Triticum aestivum

eIF5 | Eukaryotic initiation factor eIF5 in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / Translation at Agrisera AB (Antibodies for research) (AS19 4252)

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Product Information

Immunogen

Recombinant eIF5 of Triticum aestivum

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 8000 (WB)
Expected | apparent MW

49 | 50 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Lactuca sativa, Nicotiana tabacum,Triticum aestivum, Pisum sativum
Predicted reactivity Nelumbo nucifera, Triticum urartu, Zea mays
Species of your interest not listed? Contact us

Application examples

Application examples

Application example

Western blot using anti-eIF5 antibodies

Recommend incubation for 1 hr at room temperature in 5% non-fat dry milk in phosphate-buffer saline with 0.5% Tween-80 (PBST). Figure shows plant leaf extracts or wheat germ extract (5 microL of the supernatant of 50 mg liquid nitrogen powdered tissue/100 microL 2X SDS Laemli buffer containing protease inhibitor crushed with a plastic pestle in a microfuge tube and centrifuged for 10 min at 4 C.) Image shown is using Agrisera Anti-rabbit HRP (1/30,000) for 1 hr at RT in 5% non-fat dry milk in phosphate-buffer saline with 0.5% Tween-80 (PBST) and using Agrisera Ultra Bright substrate and exposed for 5 seconds in a BioRad ChemDoc on high resolution. Blue star indicates eIF5 band. Courtesy Dr. Karen Browning, The University of Texas at Austin , USA

Additional information

Related products

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Plant protein extraction buffer

Secondary antibodies

Background

Background

eIF5 (Eukaryotic initiation factor eIF5) is involved in protein biosynthesis. 

Product citations

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