eIF5 | Eukaryotic initiation factor eIF5

AS19 4252 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Triticum aestivum

eIF5 | Eukaryotic initiation factor eIF5

Product Information

Immunogen

Recombinant eIF5 of Triticum aestivum

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 200 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 8000 (WB)

Expected | apparent MW

49 | 50 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Lactuca sativa, Nicotiana tabacum,Triticum aestivum, Pisum sativum

Predicted reactivity Nelumbo nucifera, Triticum urartu, Zea mays
Species of your interest not listed? Contact us

Application examples

Application example

Recommend incubation for 1 hr at room temperature in 5% non-fat dry milk in phosphate-buffer saline with 0.5% Tween-80 (PBST). Figure shows plant leaf extracts or wheat germ extract (5 microL of the supernatant of 50 mg liquid nitrogen powdered tissue/100 microL 2X SDS Laemli buffer containing protease inhibitor crushed with a plastic pestle in a microfuge tube and centrifuged for 10 min at 4 C.) Image shown is using Agrisera Anti-rabbit HRP (1/30,000) for 1 hr at RT in 5% non-fat dry milk in phosphate-buffer saline with 0.5% Tween-80 (PBST) and using Agrisera Ultra Bright substrate and exposed for 5 seconds in a BioRad ChemDoc on high resolution. Blue star indicates eIF5 band. Courtesy Dr. Karen Browning, The University of Texas at Austin , USA

Additional information

Background

eIF5 (Eukaryotic initiation factor eIF5) is involved in protein biosynthesis.

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Picture (footer):	Application example Recommend incubation for 1 hr at room temperature in 5% non-fat dry milk in phosphate-buffer saline with 0.5% Tween-80 (PBST). Figure shows plant leaf extracts or wheat germ extract (5 microL of the supernatant of 50 mg liquid nitrogen powdered tissue/100 microL 2X SDS Laemli buffer containing protease inhibitor crushed with a plastic pestle in a microfuge tube and centrifuged for 10 min at 4 C.) Image shown is using Agrisera Anti-rabbit HRP (1/30,000) for 1 hr at RT in 5% non-fat dry milk in phosphate-buffer saline with 0.5% Tween-80 (PBST) and using Agrisera Ultra Bright substrate and exposed for 5 seconds in a BioRad ChemDoc on high resolution. Blue star indicates eIF5 band. Courtesy Dr. Karen Browning, The University of Texas at Austin , USA
background:	eIF5 (Eukaryotic initiation factor eIF5) is involved in protein biosynthesis.
Confirmed reactivity:	Arabidopsis thaliana, Lactuca sativa, Nicotiana tabacum,Triticum aestivum, Pisum sativum
predicted reactivity:	Nelumbo nucifera, Triticum urartu, Zea mays Species of your interest not listed? Contact us
calculated \| apparent molecular mass [kDa]:	49 \| 50 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	Recombinant eIF5 of Triticum aestivum
Purity:	Serum
Quantity:	50 µl
recommended dilution:	1 : 8000 (WB)
Reconstitution:	For reconstitution add 200 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)