ELF4 | Early flowering 4 (N-terminal)

Product no: AS22 4792

AS22 4792  | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Arabidopsis thaliana, recombinant ELF4

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Delivery:  3-6 business days
  • Product Info
  • Immunogen: KLH-conjugated peptide derived from N-terminal of Arabidopsis thaliana ELF4, UniProt: O04211, TAIR: AT2G40080
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Antigen affinity purified serum, in PBS pH 7.4
    Format: Lyophilized
    Quantity: 50 µg
    Reconstitution: For reconstitution, add 50 µl, of sterile water.
    Storage: Lyophilized antibody can be stored at -20°C for up to several years. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 12.37 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Not reactive in: Colobanthus apetalus, Lupinus luteus, Oryza sativa, Triticum aestivum, Vicia faba
  • Additional Information
  • Additional information (application): This antibody is recognizing a recombinant 35S::ELF4-GFP. Reactivity in endogenous sample remains to be determined.
  • Background
  • Background: ELF4 (Early flowering 4) is the component of the central CCA1/LHY-TOC1 negative feedback loop in the circadian clock that promotes clock accuracy and is required for sustained rhythms in the absence of daily light/dark cycles. Increases ELF3 nuclear distribution and localization in nuclear bodies. ELF4 is necessary for light-induced expression of both CCA1 and LHY and mediates both entrainment to an environmental cycle and circadian rhythm sustainability under constant conditions. Controls flowering time. Alternative name: Protein ARRHYTHMIC 44.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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