LFY | Leafy
AS16 3990 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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Product Information
Immunogen
KLH-conjugated synthetic peptide derived from LFY sequence of Arabidopsis thaliana, UniProt: Q00958, TAIR: AT5G61850
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Quantity
50 ĩg
Reconstitution
For reconstitution add 50 ĩl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Immunoprecipitation (IP), Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
46,5 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Amelanchier aff. bartramiana KC-2017, Arabis alpina, Bauhinia ramosissima, Coccinia racemiflora, Crataegus viridis, Fragaria nubicola, Gaultheria procumbens, Kageneckia oblonga, Neillia incisa, Piliostigma reticulatum, Physocarpus capitatus, Vauquelinia californica
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
Application examples
Application examples
Application example
20 µg of total protein: Markers (1), 35S:: LFY lines tsp1 (2), 35S:: LFY lines E1 (3), 35S:: LFY lines tsp2 (4), 35S:: LFY lines E2 (5), Empty well (6), 35S:: LFY lines tsp3 (7), 35S:: LFY lines E3 (8), LFY recombinant protein (9) extracted freshly from leaves with IP buffer (Co-IP kit from Invitrogen) and denatured with SDS reducing dye at 90°C for 5 min were separated on 12 % SDS-PAGE and blotted for 10 min to PVDF using dry transfer system (iBlot, Invitrogen). Blot was blocked with blocking buffer for 10 min at RT with agitation. Blot was incubated with primary antibody (anti-LFY, Agrisera) at a dilution of 1: 1 000, washed, incubated with Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602 Agrisera) diluted to 1:25 000 and washed. The incubations were performed for 2h 30 min using the ibind system (Invitrogen). The blot was developed for 1 min with ECL reagents (BioRad). Exposure time was 100 seconds. The red arrow indicates the position of LFY from in planta samples and the blue the position of the truncated version of recombinant LFY.
Courtesy of Dr. Claudius Marondedze, CEA, France
20 µg of total protein: Markers (1), 35S:: LFY lines tsp1 (2), 35S:: LFY lines E1 (3), 35S:: LFY lines tsp2 (4), 35S:: LFY lines E2 (5), Empty well (6), 35S:: LFY lines tsp3 (7), 35S:: LFY lines E3 (8), LFY recombinant protein (9) extracted freshly from leaves with IP buffer (Co-IP kit from Invitrogen) and denatured with SDS reducing dye at 90°C for 5 min were separated on 12 % SDS-PAGE and blotted for 10 min to PVDF using dry transfer system (iBlot, Invitrogen). Blot was blocked with blocking buffer for 10 min at RT with agitation. Blot was incubated with primary antibody (anti-LFY, Agrisera) at a dilution of 1: 1 000, washed, incubated with Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602 Agrisera) diluted to 1:25 000 and washed. The incubations were performed for 2h 30 min using the ibind system (Invitrogen). The blot was developed for 1 min with ECL reagents (BioRad). Exposure time was 100 seconds. The red arrow indicates the position of LFY from in planta samples and the blue the position of the truncated version of recombinant LFY.
Courtesy of Dr. Claudius Marondedze, CEA, France
Additional information
Additional information
Affinity purified antibodies are lyophilized from PBS pH 7,4
This antibody is recognizing LFY-YFP
Background
Background
LFY (Leafy) transcriptional regulator that promotes the transition to flowering and is involved in floral meristem development.
Product citations
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