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LFY | Leafy

AS16 3990   | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Arabidopsis thaliana
LFY | Leafy in the group Antibodies for Plant/Algal  / Developmental Biology / Flowering at Agrisera AB (Antibodies for research) (AS16 3990)

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from LFY sequence of Arabidopsis thaliana, UniProt: Q00958, TAIR: AT5G61850

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Immunoprecipitation (IP), Western blot (WB)
Recommended dilution

1 : 1000 (WB)

Expected | apparent MW

46.5 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Amelanchier aff. bartramiana KC-2017, Arabis alpina, Bauhinia ramosissima, Coccinia racemiflora, Crataegus viridis, Fragaria nubicola, Gaultheria procumbens, Kageneckia oblonga, Neillia incisa, Piliostigma reticulatum, Physocarpus capitatus, Vauquelinia californica

Species of your interest not listed? Contact us
Not reactive in  

Application examples

Application examples Application example


Western blot using anti-LFY antibodies
20 µg of total protein: Markers (1), 35S:: LFY lines tsp1 (2), 35S:: LFY lines E1 (3), 35S:: LFY lines tsp2 (4), 35S:: LFY lines E2 (5), Empty well (6), 35S:: LFY lines tsp3 (7), 35S:: LFY lines E3 (8), LFY recombinant protein (9) extracted freshly from leaves  with IP buffer (Co-IP kit from Invitrogen) and denatured with SDS reducing dye at 90°C for 5 min were separated on 12 % SDS-PAGE  and blotted for 10 min to PVDF using dry transfer system (iBlot, Invitrogen). Blot was blocked with blocking buffer for 10 min at RT with agitation. Blot was incubated with primary antibody (anti-LFY, Agrisera) at a dilution of 1: 1 000, washed, incubated with Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602 Agrisera) diluted to 1:25 000 and washed. The incubations were performed for 2h 30 min using the ibind system (Invitrogen). The blot was developed for  1  min with ECL reagents (BioRad). Exposure time was 100 seconds. The red arrow indicates the position of LFY from in planta samples and the blue the position of the truncated version of recombinant LFY.

Courtesy of Dr. Claudius Marondedze, CEA, France

Additional information

Additional information Affinity purified antibodies are lyophilized from PBS pH 7.4
This antibody is recognizing LFY-YFP.

Related products

Related products AS16 3160 | Anti-CO | Constans, goat antibodies
AS06 198 | Anti-FT/TSF | Flowering locus T and twin sister of FT, rabbit antibodies

AS11 1759 | Anti-WUS | WUSCHEL protein, rabbit antibody

Plant protein extraction buffer

Secondary antibodies

Background

Background LFY (Leafy) transcriptional regulator that promotes the transition to flowering and is involved in floral meristem development.

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