LFY | Leafy

Product no: AS16 3990
AS16 3990   | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Arabidopsis thaliana
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Delivery:  3-6 business days
  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived from LFY sequence of Arabidopsis thaliana, UniProt: Q00958, TAIR: AT5G61850

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 g
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Immunoprecipitation (IP), Western blot (WB)
    Recommended dilution:

    1 : 1000 (WB)

    Expected | apparent MW: 46,5 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Amelanchier aff. bartramiana KC-2017, Arabis alpina, Bauhinia ramosissima, Coccinia racemiflora, Crataegus viridis, Fragaria nubicola, Gaultheria procumbens, Kageneckia oblonga, Neillia incisa, Piliostigma reticulatum, Physocarpus capitatus, Vauquelinia californica

    Species of your interest not listed? Contact us
    Not reactive in:  
  • Application Examples
  • Application example

    Western blot using anti-LFY antibodies
    20 µg of total protein: Markers (1), 35S:: LFY lines tsp1 (2), 35S:: LFY lines E1 (3), 35S:: LFY lines tsp2 (4), 35S:: LFY lines E2 (5), Empty well (6), 35S:: LFY lines tsp3 (7), 35S:: LFY lines E3 (8), LFY recombinant protein (9) extracted freshly from leaves  with IP buffer (Co-IP kit from Invitrogen) and denatured with SDS reducing dye at 90°C for 5 min were separated on 12 % SDS-PAGE  and blotted for 10 min to PVDF using dry transfer system (iBlot, Invitrogen). Blot was blocked with blocking buffer for 10 min at RT with agitation. Blot was incubated with primary antibody (anti-LFY, Agrisera) at a dilution of 1: 1 000, washed, incubated with Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602 Agrisera) diluted to 1:25 000 and washed. The incubations were performed for 2h 30 min using the ibind system (Invitrogen). The blot was developed for  1  min with ECL reagents (BioRad). Exposure time was 100 seconds. The red arrow indicates the position of LFY from in planta samples and the blue the position of the truncated version of recombinant LFY.

    Courtesy of Dr. Claudius Marondedze, CEA, France
  • Additional Information
  • Additional information: Affinity purified antibodies are lyophilized from PBS pH 7,4
    Additional information (application): This antibody is recognizing LFY-YFP
  • Background
  • Background: LFY (Leafy) transcriptional regulator that promotes the transition to flowering and is involved in floral meristem development.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

    Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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AS16 ECL-S | extreme low femtogram detection

This product can be purchased in 3 different volumes:

AS16 ECL-S-10, 10 ml. Trial size limited to one per customer

AS16 ECL-S-100, 100 ml

AS16 ECL-S-1L, 1L

Choose the appropriate volume in the drop down menu to the right

From 17 €