ELF3 | Early Flowering 3

AS18 4168 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Brachypodium distachyon, Oryza sativa, Solanum tuberosum

Product Information

Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana ELF3, UniProt: O82804-1,TAIR: At2g25930

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 50 µg

Reconstitution For reconstitution add 50 µl, of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Chromatin immunoprecipitation (ChIP), Immunoprecipitation (IP), Western blot (WB)

Recommended dilution 3 µl of antibody (ChIP), 3 µl of antibody used for coating 2x20 µl Dynabeads A+G (IP), 1 : 1000 (WB)

Expected | apparent MW 77 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Brachypodium distachyon, Oryza sativa, Solanum tuberosum (leaf)

Predicted reactivity Brassica rapa, Cucurbita moschata, Corchorus olitorius, Durio zibethinus, Hevea brasiliensis, Herrania umbratica, Hordeum vulgare, Manihot esculenta, Nymphaea thermarum, Olea europaea var. sylvestris, Panicum miliaceum, Phoenix dactylifera, Pisum sativum, Populus euphratica, Prunus dulcis, Populus tremula, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Spinacia oleracea, Tanacetum cinerariifolium, Theobroma cacao, Trifolium pratense, Triticum aestivum, Zea mays, Vitis vinifera
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

ELF3 protein is extremely sensitive to light therefore extraction has to be done in green light and protein analysis has to be done using fresh extracts only. Even short light exposure cause degradation of ELF3. Extracted protein cannot be frozen but analyse directly following extraction.

Antibody is confirmed to work in ChIP for Oryza sativa Nippon bare.

Related products

Related products collection of antibodies to proteins involved in flowering

Background

Background ELF3 (Early Flowering 3) is a transcription factor, which acts within a 'zeitnehmer' feedback loop and is involved in its own circadian regulation and is a part of a corepressor complex consisting of ELF4, ELF3, and LUX involved in the transcriptional regulation of APRR9. The activity of the protein may be decreased in long day conditions due to its interaction with phytochrome B (phyB). ELF3 is also involved in responses to nematode parasitism.

Alternative names: Nematode-responsive protein

Product citations

Selected references Andrade et al. (2022) The evening complex integrates photoperiod signals to control flowering in rice. Proc Natl Acad Sci U S A. 2022 Jun 28;119(26):e2122582119. doi: 10.1073/pnas.2122582119. Epub 2022 Jun 21. PMID: 35733265; PMCID: PMC9245669.