ELF3 | Early Flowering 3
AS18 4168 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Brachypodium distachyon, Oryza sativa

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Product Information
Immunogen
KLH-conjugated peptide derived from Arabidopsis thaliana ELF3, UniProt: O82804-1,TAIR: At2g25930
Host
Rabbit
Clonality
Polyclonal
Purity
Affinity purified serum, in PBS pH 7.4
Format
Lyophilized
Quantity
50 µg
Reconstitution
For reconstitution add 50 µl, of sterile water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Chromatin immunoprecipitation (ChIP), Immunoprecipitation (IP), Western blot (WB)
Recommended dilution
3 µl of antibody (ChIP), 3 µl of antibody used for coating 2x20 µl Dynabeads A+G (IP), 1 : 1000 (WB)
Expected | apparent MW
77 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana, Brachypodium distachyon, Oryza sativa
Predicted reactivity
Brassica rapa, Cucurbita moschata, Corchorus olitorius, Durio zibethinus, Hevea brasiliensis, Herrania umbratica, Hordeum vulgare, Manihot esculenta, Nymphaea thermarum, Olea europaea var. sylvestris, Panicum miliaceum, Phoenix dactylifera, Pisum sativum, Populus euphratica, Prunus dulcis, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Spinacia oleracea, Tanacetum cinerariifolium, Theobroma cacao, Trifolium pratense, Triticum aestivum, Zea mays, Vitis vinifera
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Additional information
ELF3 protein is extremely sensitive to light therefore extraction has to be done in green light and protein analysis has to be done using fresh extracts only. Even short light exposure cause degradation of ELF3. Extracted protein cannot be frozen but analyse directly following extraction.
Antibody is confirmed to work in ChIP for Oryza sativa Nippon bare.
Antibody is confirmed to work in ChIP for Oryza sativa Nippon bare.
Background
Background
ELF3 (Early Flowering 3) is a transcription factor, which acts within a 'zeitnehmer' feedback loop and is involved in its own circadian regulation and is a part of a corepressor complex consisting of ELF4, ELF3, and LUX involved in the transcriptional regulation of APRR9. The activity of the protein may be decreased in long day conditions due to its interaction with phytochrome B (phyB). ELF3 is also involved in responses to nematode parasitism.
Alternative names: Nematode-responsive protein
Alternative names: Nematode-responsive protein
Product citations
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