Name:
Phone:
E-mail:
Message


 


ELF3 | Early Flowering 3

AS18 4168   | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Arabidopsis thaliana, Brachypodium distachyon, Oryza sativa

ELF3 | Early Flowering 3 in the group Antibodies for Plant/Algal  / Developmental Biology / Flowering at Agrisera AB (Antibodies for research) (AS18 4168)

DATA SHEET IN PDF

Qty: 
200
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana ELF3, UniProt: O82804-1,TAIR: At2g25930
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum, in PBS pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl, of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.

Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Chromatin immunoprecipitation (ChIP), Immunoprecipitation (IP), Western blot (WB)
Recommended dilution 3 µl of antibody  (ChIP), 3 µl of antibody used for coating 2x20 µl Dynabeads A+G (IP), 1 : 1000 (WB)
Expected | apparent MW 77 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Brachypodium distachyon, Oryza sativa
Predicted reactivity Brassica rapa, Cucurbita moschata, Corchorus olitorius, Durio zibethinus, Hevea brasiliensis, Herrania umbratica, Hordeum vulgare, Manihot esculenta, Nymphaea thermarum, Olea europaea var. sylvestris, Panicum miliaceum, Phoenix dactylifera, Pisum sativum, Populus euphratica, Prunus dulcis, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Spinacia oleracea, Tanacetum cinerariifolium, Theobroma cacao, Trifolium pratense, Triticum aestivum, Zea mays, Vitis vinifera
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

ELF3 protein is extremely sensitive to light therefore extraction has to be done in green light and protein analysis has to be done using fresh extracts only. Even short light exposure cause degradation of ELF3. Extracted protein cannot be frozen but analyse directly following extraction.

Antibody is confirmed to work in ChIP for Oryza sativa Nippon bare

Related products

Background

Background ELF3 (Early Flowering 3) is a transcription factor, which acts within a 'zeitnehmer' feedback loop and is involved in its own circadian regulation and is a part of a corepressor complex consisting of ELF4, ELF3, and LUX involved in the transcriptional regulation of APRR9. The activity of the protein may be decreased in long day conditions due to its interaction with phytochrome B (phyB). ELF3 is also involved in responses to nematode parasitism.

Alternative names: Nematode-responsive protein

Product citations

Related products: ELF3 | Early Flowering 3

AS16 ECL-S | extreme low femtogram detection
- 10ml trial size is restricted to one unit per cust...
From 10 €
AS06 198 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
286 €
Buy 2 items of this product for 214.00 €/items
Buy 3 items of this product for 195.00 €/items
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
190 €
AS16 3990   | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Arabidopsis ...
286 €