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EPYC1 | Essential Pyrenoid Component 1
AS19 4312 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii
- Product Info
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Immunogen: KLH-conjugated peptide derived from Chlamydomonas reinhardtii EPYC1, C-terminal part UniProt: A0A2K3DA85 Host: Rabbit
Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4. Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution add 50 µl, of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 32 kDa - Reactivity
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Confirmed reactivity: Chlamydomonas reinhardti Predicted reactivity: Chlamydomonas reinhardtii Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Samples:
1 – 25 µg of Chlamydomonas reinhardtii wild-type grown in low light.
2 – 25 µg of Chlamydomonas reinhardtii epyc1 mutant grown in low light.
3 – 25 µg of Chlamydomonas reinhardtii wild-type incubated 2 hours in high light.
4 – 25 µg of Chlamydomonas reinhardtii epyc1 mutant incubated 2 hours in high light.
Markers: Precision Plus Protein Dual Color from Biorad.25 µg/well of total protein extracted freshly from Chlamydomonas reinhardtii cells with 0.1mM NaOH + Protease inhibitor cocktail set IV (1:200; 539136, Sigma) and denatured with Sample buffer (1.6 mM EDTA, 1.6% SDS, 40 mM DTT, 8% Glycerol, 0.016% Bromophenol Blue, 333 mM Tris Base) at 70°C for 5 min, were separated on 12% SDS-PAGE and blotted 1h to nitrocellulose membrane (pore size of 0.2 um), using semi-dry transfer. Blot was blocked with 5% milk for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 in TBS-T 5% milk ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602) diluted to 1:25 000 in TBS-T 5% milk for 1h/RT with agitation. The blot was washed as above and developed for 5 min with Agrisera ECL Bright. Exposure time was 10 seconds.
Courtesy of Dr. Justin Findinier, Carnegie Institution for Science, USA - Background
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Background: EPYC1 (Essential Pyrenoid Component 1), also known as Lci5, is localizing in the pyrenoid matrix, is of similar abundance as Rubisco and its function is scaffolding for the assembly of Rubisco in the pyrenoid.
- Product Citations
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Selected references: Burlacot et al. (2022) Alternative photosynthesis pathways drive the algal CO2-concentrating mechanism. Nature 605, 366–371 (2022). https://doi.org/10.1038/s41586-022-04662-9
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Poster Collection - Reviews:
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
10 % until end of 2023. Use discount code: Conj10
AS03 037 | Clonality: Polyclonal | Host: Rabbit | Reactivity: global antibody and compartment marker for higher plants, lichens, algae, cyanobacteria, dinoflagellates, diatoms
Benefits of using this antibody