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EPYC1 | Essential Pyrenoid Component 1

AS19 4312   | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Chlamydomonas reinhardtii

EPYC1 | Essential Pyrenoid Component 1 in the group Antibodies for Plant/Algal  / Photosynthesis  / RUBISCO/Carbon metabolism at Agrisera AB (Antibodies for research) (AS19 4312)

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Product Information

Immunogen KLH-conjugated peptide derived from Chlamydomonas reinhardtii EPYC1, C-terminal part UniProt: A0A2K3DA85
Host

Rabbit

Clonality Polyclonal
Purity Affinity purified serum, in PBS pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl, of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW 32 kDa

Reactivity

Confirmed reactivity Chlamydomonas reinhardti
Predicted reactivity Chlamydomonas reinhardtii
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Western blot using anti-EPYC1 antibodies

Samples:
1 – 25 µg of Chlamydomonas reinhardtii wild-type grown in low light.
2 – 25 µg of Chlamydomonas reinhardtii epyc1 mutant grown in low light.
3 – 25 µg of Chlamydomonas reinhardtii wild-type incubated 2 hours in high light.
4 – 25 µg of Chlamydomonas reinhardtii epyc1 mutant incubated 2 hours in high light.
Markers: Precision Plus Protein Dual Color from Biorad.

25 µg/well of total protein extracted freshly from Chlamydomonas reinhardtii cells with 0.1mM NaOH + Protease inhibitor cocktail set IV (1:200; 539136, Sigma) and denatured with Sample buffer (1.6 mM EDTA, 1.6% SDS, 40 mM DTT, 8% Glycerol, 0.016% Bromophenol Blue, 333 mM Tris Base) at 70°C for 5 min, were separated on 12% SDS-PAGE and blotted 1h to nitrocellulose membrane (pore size of 0.2 um), using semi-dry transfer. Blot was blocked with 5% milk for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 in TBS-T 5% milk ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602) diluted to 1:25 000 in TBS-T 5% milk for 1h/RT with agitation. The blot was washed as above and developed for 5 min with Agrisera ECL Bright. Exposure time was 10 seconds.
Courtesy of Dr. Justin Findinier, Carnegie Institution for Science, USA

Additional information

Related products

Background

Background EPYC1 (Essential Pyrenoid Component 1), also known as Lci5, is localizing in the pyrenoid matrix, is of similar abundance as Rubisco and its function is scaffolding for the assembly of Rubisco in the pyrenoid.

Product citations

Selected references to be added when available, antibody released in January 2021.

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