FBA | Fructose-bisphosphate aldolase class 2

Product no: AS13 2731

AS13 2731  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Synechocystis PCC6803

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  • Product Info
  • Immunogen:

    Recombinant FBA from Synechocystis sp. PCC6803, UniProt: Q55664, Cyanobase: sll0018

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 l
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 38,9 kDa
  • Reactivity
  • Confirmed reactivity: Synechocystis PCC6803
    Predicted reactivity: Cyanobacteria
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • application example
     
    western blot using anti-FBA antibodies

    From 0.15 to 5.17 µg of total protein from Synechocystis PCC6803 (A) extracted with SDS-sample buffer and respective amounts of recombinant FBA (B) were separated on 15 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5 % milk powder in TBS-T  for 30 min. at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 7 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 2 min with ECL according to the manufacturer's instructions. Exposure time was  seconds.

    Courtesy of Yichen Zhang, Department of Biochemistry and Molecular Biology, University of Massachusetts, USA

     

  • Additional Information
  • Additional information: This antibody can be used as a marker of cytoplasmic fraction in cyanobacteria
  • Background
  • Background: FBP aldolase (FBP) is an enzyme (EC=4.1.2.13) which is catalyzing the aldol condensation of of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis. It belongs to class II fructose-bisphosphate aldolase family.

    Alternative names: FBPA, fructose-1,6-bisphosphate aldolase, fructose-bisphosphate aldolase class II.
  • Protocols


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