FBA | Fructose-bisphosphate aldolase class 2
AS13 2731 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Synechocystis PCC6803
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From 0.15 to 5.17 µg of total protein from Synechocystis PCC6803 (A) extracted with SDS-sample buffer and respective amounts of recombinant FBA (B) were separated on 15 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5 % milk powder in TBS-T for 30 min. at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 7 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 2 min with ECL according to the manufacturer's instructions. Exposure time was seconds.
Courtesy of Yichen Zhang, Department of Biochemistry and Molecular Biology, University of Massachusetts, USA
Alternative names: FBPA, fructose-1,6-bisphosphate aldolase, fructose-bisphosphate aldolase class II.