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FBA | Fructose-bisphosphate aldolase class 2

AS13 2731  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Synechocystis PCC6803

FBA | Fructose-bisphosphate aldolase class 2 in the group Antibodies for Plant/Algal  / Carbohydrates at Agrisera AB (Antibodies for research) (AS13 2731)

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Product Information

Immunogen

Recombinant FBA from Synechocystis sp. PCC6803, UniProt: Q55664, Cyanobase: sll0018

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

38.9 kDa

Reactivity

Confirmed reactivity Synechocystis PCC6803
Predicted reactivity Cyanobacteria
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

application example
 
western blot using anti-FBA antibodies

From 0.15 to 5.17 µg of total protein from Synechocystis PCC6803 (A) extracted with SDS-sample buffer and respective amounts of recombinant FBA (B) were separated on 15 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5 % milk powder in TBS-T  for 30 min. at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 7 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 2 min with ECL according to the manufacturer's instructions. Exposure time was  seconds.

Courtesy of Yichen Zhang, Department of Biochemistry and Molecular Biology, University of Massachusetts, USA

 

Additional information

Additional information This antibody can be used as a marker of cytoplasmic fraction in cyanobacteria.

Related products

Related products

AS08 294 | Anti-ALD | fructose-1,6 bisphosphate aldolase, rabbit antibodies

AS16 3848 | Anti-FBA | Fructose-bisphosphate aldolase 1, cytoplasmic, rabbit antibodies


collection of antibodies to proteins involved in carbohydrate metabolism

Algal protein extraction buffer

Secondary antibodies

Background

Background FBP aldolase (FBP) is an enzyme (EC=4.1.2.13) which is catalyzing the aldol condensation of of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis. It belongs to class II fructose-bisphosphate aldolase family.

Alternative names: FBPA, fructose-1,6-bisphosphate aldolase, fructose-bisphosphate aldolase class II.

Product citations

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