FBPase1 | Fructose-1,6-bisphosphatase 1, chloroplastic (chloroplastic marker in photosynthetic tissues)

Product no: AS19 4319

AS19 4319   | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Arabidopsis thaliana

Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items
319
Add to cart
Delivery:  3-6 business days
  • Product Info
  • Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana FBPase1 (chloroplastic), UniProt: P25851-1, TAIR: AT3G54050
    Host:

    Rabbit

    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 g
    Reconstitution: For reconstitution add 50 l, of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 50 000 (WB)
    Expected | apparent MW: 45 | 48 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Abrus precatorius, Actinidia chinensis, Arabis nemorensis, Beta vulgaris, Brassica napus, Capsella rubella, Cephalotus follicularis, Eucalyptus grandis, Gossypium tomentosum, Hibiscus syriacus, Manihot esculenta, Morella rubra, Mucuna pruriens, Nelumbo nucifera, Parasponia andersonii, Populus sp., Prunus dulcis, Prunus persica, Salvia splendens, Syzygium oleaosum, Vitis vinifera
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • application example


    western blot using anti-FBPase (chloroplastic) antibodies


    20 μg/well of total protein of Arabidopsis thaliana wildtype (1), chloroplastic FBPase mutant (2), mutant line of a different gene (3) were freshly extracted from 8-week old leaves with buffer (50 mM Tris-HCl pH8.0, 200 mM NaCl, 10 mM DTT (dithiothreitol), 1% (v/v) Triton X-100, Sigma protease inhibitor cocktail) and denatured with 4X SDS buffer at 95°C for 5 min. Samples were separated on 10% SDS-PAGE and blotted 1h to PVDF membrane (pore size of 0.2 um), using wet transfer. Blot was blocked with 5% milk for 0.5h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 50, 000 (from the initial reconstituded antibody solution at 1 µg IgG/µl) in PBS-T for ON at 4°C with agitation. The antibody solution was decanted and the blot was washed 4 times for 10 min in PBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602, Agrisera) diluted to 1:25 000 in for 1h/RT with agitation. The blot was washed as above and developed for 1 min with Agrisera ECL SuperBright, AS16 ECL-S. Exposure time was 2 mins.

    Courtesy Dr Hong Wang, University of Saskatchewan, Canada
  • Background
  • Background: FBPase1 (Fructose-1,6-bisphosphatase 1, chloroplastic (chloroplastic marker in photosynthetic tissues) involved in carbohydrate biosynthesis. 

    Alternative names:  D-fructose-1,6-bisphosphate 1-phosphohydrolase, HCEF1 (High Cyclic Electron Flow 1).
  • Product Citations
  • Selected references: Penzler et al. (2022) Commonalities and specialties in photosynthetic functions of PROTON GRADIENT REGULATION5 variants in Arabidopsis. Plant Physiol. 2022;190(3):1866-1882. doi:10.1093/plphys/kiac362
    Wang et al. (2022), Arabidopsis Ubiquitin-Conjugating Enzymes UBC4, UBC5, and UBC6 Have Major Functions in Sugar Metabolism and Leaf Senescence, Int. J. Mol. Sci. 2022, 23(19), 11143; https://doi.org/10.3390/ijms231911143
    Lim et al (2022). Arabidopsis guard cell chloroplasts import cytosolic ATP for starch turnover and stomatal opening. Nat Commun. 2022 Feb 3;13(1):652. doi: 10.1038/s41467-022-28263-2. PMID: 35115512; PMCID: PMC8814037.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Poster Collection
  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
    Your name will be displayed as the sender.
    Number of reviews: (0)

    This product doesn't have any reviews.

Accessories

AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

213 €
Add to cart
Info

AS04 043  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana, Brassica napus, Macroptilium atropurpureum, Nicotiana benthamiana, Pinus silvestris, Pinus yunanniensis, Oryza sativa, Petunia hybrida cv. Mitchell, Solanum tuberosum, Zea mays |  cellular [compartment marker] of cytoplasm 

319 €
Add to cart
Info
Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items

AS05 086  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, C. annuum, C. sativus, F. margarita Swingle, F. arundinacea,  H. vulgare, L. esculentum, L. chilense, Malus x domestica Borkh. c.v. Fuji, M. polymorpha, Medicago truncatula, N. tabacum, O. sativa, P. glauca, Populus sp., S. tuberosum, S. sogarandinum, Triticum |  cellular [compartment marker] of cytoplasm

Replaced by AS14 2813 

Close