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FBPase1 | Fructose-1,6-bisphosphatase 1, chloroplastic (chloroplastic marker in photosynthetic tissues)

AS19 4319   | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Arabidopsis thaliana

FBPase1 | Fructose-1,6-bisphosphatase 1, chloroplastic (chloroplastic marker in photosynthetic tissues) in the group Antibodies for Plant/Algal  / Arabidopsis thaliana  at Agrisera AB (Antibodies for research) (AS19 4319)

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Product Information

Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana FBPase1 (chloroplastic), UniProt: P25851-1, TAIR: AT3G54050
Host

Rabbit

Clonality Polyclonal
Purity Affinity purified serum, in PBS pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl, of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 50 000 (WB)
Expected | apparent MW 45 | 48 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Abrus precatorius, Actinidia chinensis, Arabis nemorensis, Beta vulgaris, Brassica napus, Capsella rubella, Cephalotus follicularis, Eucalyptus grandis, Gossypium tomentosum, Hibiscus syriacus, Manihot esculenta, Morella rubra, Mucuna pruriens, Nelumbo nucifera, Parasponia andersonii, Populus sp., Prunus dulcis, Prunus persica, Salvia splendens, Syzygium oleaosum, Vitis vinifera
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example


western blot using anti-FBPase (chloroplastic) antibodies


20 μg/well of total protein of Arabidopsis thaliana wildtype (1), chloroplastic FBPase mutant (2), mutant line of a different gene (3) were freshly extracted from 8-week old leaves with buffer (50 mM Tris-HCl pH8.0, 200 mM NaCl, 10 mM DTT (dithiothreitol), 1% (v/v) Triton X-100, Sigma protease inhibitor cocktail) and denatured with 4X SDS buffer at 95°C for 5 min. Samples were separated on 10% SDS-PAGE and blotted 1h to PVDF membrane (pore size of 0.2 um), using wet transfer. Blot was blocked with 5% milk for 0.5h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 50, 000 (from the initial reconstituded antibody solution at 1 µg IgG/µl) in PBS-T for ON at 4°C with agitation. The antibody solution was decanted and the blot was washed 4 times for 10 min in PBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602, Agrisera) diluted to 1:25 000 in for 1h/RT with agitation. The blot was washed as above and developed for 1 min with Agrisera ECL SuperBright, AS16 ECL-S. Exposure time was 2 mins.

Courtesy Dr Hong Wang, University of Saskatchewan, Canada

Additional information

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Background

Background FBPase1 (Fructose-1,6-bisphosphatase 1, chloroplastic (chloroplastic marker in photosynthetic tissues) involved in carbohydrate biosynthesis. 

Alternative names:  D-fructose-1,6-bisphosphate 1-phosphohydrolase, HCEF1 (High Cyclic Electron Flow 1).

Product citations

Related products: FBPase1 | Fructose-1,6-bisphosphatase 1, chloroplastic (chloroplastic marker in photosynthetic tissues)

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