FBPase1 | Fructose-1,6-bisphosphatase 1, chloroplastic (chloroplastic marker in photosynthetic tissues)

AS19 4319 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

FBPase1 | Fructose-1,6-bisphosphatase 1, chloroplastic (chloroplastic marker in photosynthetic tissues)

Product Information

Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana FBPase1 (chloroplastic), UniProt: P25851-1, TAIR: AT3G54050

Host

Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 50 µg

Reconstitution For reconstitution add 50 µl, of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 50 000 (WB)

Expected | apparent MW 45 | 48 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Abrus precatorius, Actinidia chinensis, Arabis nemorensis, Beta vulgaris, Brassica napus, Capsella rubella, Cephalotus follicularis, Eucalyptus grandis, Gossypium tomentosum, Hibiscus syriacus, Manihot esculenta, Morella rubra, Mucuna pruriens, Nelumbo nucifera, Parasponia andersonii, Populus sp., Prunus dulcis, Prunus persica, Salvia splendens, Syzygium oleaosum, Vitis vinifera
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example

western blot using anti-FBPase (chloroplastic) antibodies

20 μg/well of total protein of Arabidopsis thaliana wildtype (1), chloroplastic FBPase mutant (2), mutant line of a different gene (3) were freshly extracted from 8-week old leaves with buffer (50 mM Tris-HCl pH8.0, 200 mM NaCl, 10 mM DTT (dithiothreitol), 1% (v/v) Triton X-100, Sigma protease inhibitor cocktail) and denatured with 4X SDS buffer at 95°C for 5 min. Samples were separated on 10% SDS-PAGE and blotted 1h to PVDF membrane (pore size of 0.2 um), using wet transfer. Blot was blocked with 5% milk for 0.5h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 50, 000 (from the initial reconstituded antibody solution at 1 µg IgG/µl) in PBS-T for ON at 4°C with agitation. The antibody solution was decanted and the blot was washed 4 times for 10 min in PBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602, Agrisera) diluted to 1:25 000 in for 1h/RT with agitation. The blot was washed as above and developed for 1 min with Agrisera ECL SuperBright, AS16 ECL-S. Exposure time was 2 mins.

Courtesy Dr Hong Wang, University of Saskatchewan, Canada

Additional information

Background

Background FBPase1 (Fructose-1,6-bisphosphatase 1, chloroplastic (chloroplastic marker in photosynthetic tissues) involved in carbohydrate biosynthesis.

Alternative names: D-fructose-1,6-bisphosphate 1-phosphohydrolase, HCEF1 (High Cyclic Electron Flow 1).

Product citations

Selected references Penzler et al. (2022) Commonalities and specialties in photosynthetic functions of PROTON GRADIENT REGULATION5 variants in Arabidopsis. Plant Physiol. 2022;190(3):1866-1882. doi:10.1093/plphys/kiac362
Wang et al. (2022), Arabidopsis Ubiquitin-Conjugating Enzymes UBC4, UBC5, and UBC6 Have Major Functions in Sugar Metabolism and Leaf Senescence, Int. J. Mol. Sci. 2022, 23(19), 11143; https://doi.org/10.3390/ijms231911143
Lim et al (2022). Arabidopsis guard cell chloroplasts import cytosolic ATP for starch turnover and stomatal opening. Nat Commun. 2022 Feb 3;13(1):652. doi: 10.1038/s41467-022-28263-2. PMID: 35115512; PMCID: PMC8814037.

All references:	Penzler et al. (2022) Commonalities and specialties in photosynthetic functions of PROTON GRADIENT REGULATION5 variants in Arabidopsis. Plant Physiol. 2022;190(3):1866-1882. doi:10.1093/plphys/kiac362 Wang et al. (2022), Arabidopsis Ubiquitin-Conjugating Enzymes UBC4, UBC5, and UBC6 Have Major Functions in Sugar Metabolism and Leaf Senescence, Int. J. Mol. Sci. 2022, 23(19), 11143; https://doi.org/10.3390/ijms231911143 Lim et al (2022). Arabidopsis guard cell chloroplasts import cytosolic ATP for starch turnover and stomatal opening. Nat Commun. 2022 Feb 3;13(1):652. doi: 10.1038/s41467-022-28263-2. PMID: 35115512; PMCID: PMC8814037.
background:	FBPase1 (Fructose-1,6-bisphosphatase 1, chloroplastic (chloroplastic marker in photosynthetic tissues) involved in carbohydrate biosynthesis. Alternative names: D-fructose-1,6-bisphosphate 1-phosphohydrolase, HCEF1 (High Cyclic Electron Flow 1).
Confirmed reactivity:	Arabidopsis thaliana
predicted reactivity:	Abrus precatorius, Actinidia chinensis, Arabis nemorensis, Beta vulgaris, Brassica napus, Capsella rubella, Cephalotus follicularis, Eucalyptus grandis, Gossypium tomentosum, Hibiscus syriacus, Manihot esculenta, Morella rubra, Mucuna pruriens, Nelumbo nucifera, Parasponia andersonii, Populus sp., Prunus dulcis, Prunus persica, Salvia splendens, Syzygium oleaosum, Vitis vinifera Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
calculated \| apparent molecular mass [kDa]:	45 \| 48 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	KLH-conjugated peptide derived from Arabidopsis thaliana FBPase1 (chloroplastic), UniProt: P25851-1, TAIR: AT3G54050
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Quantity:	50 µg
recommended dilution:	1 : 50 000 (WB)
Reconstitution:	For reconstitution add 50 µl, of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
Picture (footer):	application example 20 μg/well of total protein of Arabidopsis thaliana wildtype (1), chloroplastic FBPase mutant (2), mutant line of a different gene (3) were freshly extracted from 8-week old leaves with buffer (50 mM Tris-HCl pH8.0, 200 mM NaCl, 10 mM DTT (dithiothreitol), 1% (v/v) Triton X-100, Sigma protease inhibitor cocktail) and denatured with 4X SDS buffer at 95°C for 5 min. Samples were separated on 10% SDS-PAGE and blotted 1h to PVDF membrane (pore size of 0.2 um), using wet transfer. Blot was blocked with 5% milk for 0.5h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 50, 000 (from the initial reconstituded antibody solution at 1 µg IgG/µl) in PBS-T for ON at 4°C with agitation. The antibody solution was decanted and the blot was washed 4 times for 10 min in PBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602, Agrisera) diluted to 1:25 000 in for 1h/RT with agitation. The blot was washed as above and developed for 1 min with Agrisera ECL SuperBright, AS16 ECL-S. Exposure time was 2 mins. Courtesy Dr Hong Wang, University of Saskatchewan, Canada