FIE | Fertilization-independent endosperm

AS12 2616 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

FIE | Fertilization-independent endosperm

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana FIE protein sequence, UniProt: Q9LT47, TAIR: At3g20740

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW

41 kDa

Reactivity

Confirmed reactivity Recombinant FIE protein

Predicted reactivity Arabidopsis thaliana, Solanum lycopersicum, Triticum aestivum
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

This antibody so far has been confirmed to detect recombinant FIE protein, GFP-tagged.

Optimization of protein extraction and detection using most sensitive reagents may result in recognition of endogenous FIE.

Background

Background FIE (fertilization-independent endosperm) belongs to polycomb group (PcG) protein which act by forming multiprotein complexes, required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development.

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Confirmed reactivity:	Recombinant FIE protein
predicted reactivity:	Arabidopsis thaliana, Solanum lycopersicum, Triticum aestivum Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
background:	FIE (fertilization-independent endosperm) belongs to polycomb group (PcG) protein which act by forming multiprotein complexes, required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development.
additional information (application):	This antibody so far has been confirmed to detect recombinant FIE protein, GFP-tagged. Optimization of protein extraction and detection using most sensitive reagents may result in recognition of endogenous FIE.
calculated \| apparent molecular mass [kDa]:	41 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	KLH-conjugated synthetic peptide derived from Arabidopsis thaliana FIE protein sequence, UniProt: Q9LT47, TAIR: At3g20740
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Quantity:	50 µg
recommended dilution:	1 : 1000 (WB)
Reconstitution:	For reconstitution add 50 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)