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FIE | Fertilization-independent endosperm

AS12 2616 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

FIE | Fertilization-independent endosperm  in the group Antibodies for Plant/Algal  / Developmental Biology / Signal transduction at Agrisera AB (Antibodies for research) (AS12 2616)

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana FIE protein sequence, UniProt: Q9LT47, TAIR: At3g20740

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

41 kDa

Reactivity

Confirmed reactivity Recombinant FIE protein
Predicted reactivity Arabidopsis thaliana, Triticum aestivum
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

 

Additional information

This antibody so far has been confirmed to detect recombinant FIE protein, GFP-tagged. 

Optimization of protein extraction and detection using most sensitive reagents may result in recognition of endogenous FIE.

Related products

Background

Background FIE (fertilization-independent endosperm) belongs to polycomb group (PcG) protein which act by forming multiprotein complexes, required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development.

Product citations

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