AHK2 | Histidine kinase 2

Product no: AS12 2113

AS12 2113 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived from Arabidopsis thaliana AHK2 protein sequence, TAIR: AT5G35750, UniProt: Q9C5U2

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 g
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW:

    131 kDa

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Nicotiana benthamiana, Vitis vinifera
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • application information

    western blot using anti-AHK2 antibodies


    The Arabidopsis thaliana wild-type (WS) and ahk2-2 T-DNA insertion mutant plants were grown on MS/2 media for six days. Tissue was collected, frozen in liquid nitrogen, ground in 3 volumes of 2x SDS-PAGE loading buffer and heated at 95 C for 5 min. After 10 min centrifugation at RT, 20 µl of the supernatant was loaded per lane on a 7.5% TGX gel (BioRad). After separation, proteins were blotted 1 hr onto a supported nitrocellulose membrane. Blots were blocked with  10% milk in PBS-T for 15 min at room temperature (RT) with agitation. Blot was incubated in the primary antibody diluted 1: 10, 000 in 1% milk in PBS-T  for 16h at 4C  with agitation. The antibody solution was decanted and the blot was washed 3 times for1 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1: 2000 in PBS-T for 1h at RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection reagent according to the manufacturer\s instructions. Chemiluminescent signal was captured using ChemiDoc equipped with a CCD camera.

    Courtesy of Dr. Jasmina Kurepa, University of Kentucky College of Agriculture, Food and Environment, USA

     

  • Background
  • Background:

    AHK2 (Histidine kinase 2) is a cytokinin receptor, multi-pass membrane protein which transmits the stress signal to a downstream MAPK cascade.

    Alternative name: Authentic HIS-Kinase 2. 

  • Product Citations
  • Selected references: 38221900
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AS12 2119  |  Clonality: Polyclonal   |  Host: Rabbit   |  Reactivity: A. platanoides, A. thaliana, C.australis R.Br., L. longiflorum. M. crystallinum, N. tabacum, O. sativa, P. kurroa, P. vulgaris, algae N. oloabundans, Z.mays

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Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items
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