AHK2 | Histidine kinase 2

AS12 2113 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

AHK2 | Histidine kinase 2 in the group Plant/Algal Antibodies / Developmental Biology / Signal transduction at Agrisera AB (Antibodies for research) (AS12 2113)


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product information

AHK2 (Histidine kinase 2) is a cytokinin receptor, multi-pass membrane protein which transmits the stress signal to a downstream MAPK cascade. Alternative name: Authentic HIS-Kinase 2. 


KLH-conjugated synthetic peptide derived from Arabidopsis thaliana AHK2 protein sequence, TAIR: AT5G35750, UniProt: Q9C5U2

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
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Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

131 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Vitis vinifera
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references

to be added when available, antibody released in July 2013.

application information

western blot using anti-AHK2 antibodies

The Arabidopsis thaliana wild-type (WS) and ahk2-2 T-DNA insertion mutant plants were grown on MS/2 media for six days. Tissue was collected, frozen in liquid nitrogen, ground in 3 volumes of 2x SDS-PAGE loading buffer and heated at 95 C for 5 min. After 10 min centrifugation at RT, 20 µl of the supernatant was loaded per lane on a 7.5% TGX gel (BioRad). After separation, proteins were blotted 1 hr onto a supported nitrocellulose membrane. Blots were blocked with  10% milk in PBS-T for 15 min at room temperature (RT) with agitation. Blot was incubated in the primary antibody diluted 1: 10, 000 in 1% milk in PBS-T  for 16h at 4C  with agitation. The antibody solution was decanted and the blot was washed 3 times for1 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1: 2000 in PBS-T for 1h at RT with agitation. The blot was washed as above and developed for 5 min with SuperSignal West Femto Substrate according to the manufacturers instructions. Chemiluminescent signal was captured using ChemiDoc equipped with a CCD camera.

Courtesy of Dr. Jasmina Kurepa, University of Kentucky College of Agriculture, Food and Environment, USA


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