AHK2 | Histidine kinase 2

AS12 2113 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

AHK2 | Histidine kinase 2 in the group Antibodies Plant/Algal  / Developmental Biology / Signal transduction at Agrisera AB (Antibodies for research) (AS12 2113)
AHK2 | Histidine kinase 2


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Product Information


KLH-conjugated synthetic peptide derived from Arabidopsis thaliana AHK2 protein sequence, TAIR: AT5G35750, UniProt: Q9C5U2

Host Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

131 kDa


Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Nicotiana benthamiana, Vitis vinifera
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples

application information

western blot using anti-AHK2 antibodies

The Arabidopsis thaliana wild-type (WS) and ahk2-2 T-DNA insertion mutant plants were grown on MS/2 media for six days. Tissue was collected, frozen in liquid nitrogen, ground in 3 volumes of 2x SDS-PAGE loading buffer and heated at 95 C for 5 min. After 10 min centrifugation at RT, 20 µl of the supernatant was loaded per lane on a 7.5% TGX gel (BioRad). After separation, proteins were blotted 1 hr onto a supported nitrocellulose membrane. Blots were blocked with  10% milk in PBS-T for 15 min at room temperature (RT) with agitation. Blot was incubated in the primary antibody diluted 1: 10, 000 in 1% milk in PBS-T  for 16h at 4C  with agitation. The antibody solution was decanted and the blot was washed 3 times for1 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1: 2000 in PBS-T for 1h at RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection reagent according to the manufacturer\s instructions. Chemiluminescent signal was captured using ChemiDoc equipped with a CCD camera.

Courtesy of Dr. Jasmina Kurepa, University of Kentucky College of Agriculture, Food and Environment, USA


Additional information

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AHK2 (Histidine kinase 2) is a cytokinin receptor, multi-pass membrane protein which transmits the stress signal to a downstream MAPK cascade.

Alternative name: Authentic HIS-Kinase 2. 

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