FNR | Ferredoxin NADP Reductase (Plasmodium falciparum)
AS20 4435 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Plasmodium falciparum
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Product Information
Immunogen
Purified full length, tag cleaved, recombinant Plasmodium falciparum FNR, UniProt: C6KT68
Host
Rabbit
Clonality
Polyclonal
Purity
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Format
Liquid at 4 mg/ml.
Quantity
400 ĩg
Storage
Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
ELISA (ELISA), Western blot (WB)
Recommended dilution
1: 500 - 1: 2000 (WB)
Expected | apparent MW
43,8 | 38 kDa (transit peptide removed)
Reactivity
Confirmed reactivity
Plasmodium falciparum
Application examples
Application examples
1 μl of 40 μM recombinant pf FNR from Plasmodium falciparum with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE and denatured with 4X SDS buffer at 95°C for 5 min. Samples were separated on 10% SDS-PAGE and blotted 1h to PVDF membrane. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendation.
Calculated MW of FNR is: 43,8 kDa. However, transit peptide consisting of N-terminal 18 amino acids is removed in the mature form.
1 μl of 40 μM recombinant pf FNR from Plasmodium falciparum with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE and denatured with 4X SDS buffer at 95°C for 5 min. Samples were separated on 10% SDS-PAGE and blotted 1h to PVDF membrane. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendation.
Calculated MW of FNR is: 43,8 kDa. However, transit peptide consisting of N-terminal 18 amino acids is removed in the mature form.
Additional information
For western blot apicoplast fraction from Plasmodium falciparum is recommended, not a total cell extract.
Background
Background
FNR | Ferredoxin NADP Reductase plays a key role in regulating the relative amounts of cyclic and non-cyclic electron flow to meet the demands of the plant for ATP and reducing power. The human malaria parasite (Plasmodium falciparum) possesses a plastid-derived organelle called the apicoplast, which is believed to employ metabolisms crucial for the parasite's survival.
Alternative name: Fd:NADPH oxidoreducatase (FNR)
Alternative name: Fd:NADPH oxidoreducatase (FNR)
Product citations
Selected references
Kimata-Ariga et al. (2007). Cloning and Characterization of Ferredoxin and ferredoxin-NADP+ Reductase From Human Malaria Parasite. J Biochem. 2007 Mar;141(3):421-8. doi: 10.1093/jb/mvm046.
Kimata-Ariga et al. (2007). Cloning and Characterization of Ferredoxin and ferredoxin-NADP+ Reductase From Human Malaria Parasite. J Biochem. 141(3):421-8. doi: 10.1093/jb/mvm046.
Kimata-Ariga et al. (2007). Cloning and Characterization of Ferredoxin and ferredoxin-NADP+ Reductase From Human Malaria Parasite. J Biochem. 141(3):421-8. doi: 10.1093/jb/mvm046.
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