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FNR3 | Ferredoxin NADP Reductase, isoprotein 3 (leaf)

AS20 4437 | Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Arabidopsis thaliana, Zea mays
FNR3 | Ferredoxin  NADP Reductase, isoprotein 3 (leaf) in the group Antibodies Plant/Algal  / Photosynthesis  / Electron transfer at Agrisera AB (Antibodies for research) (AS20 4437)
FNR3 | Ferredoxin  NADP Reductase, isoprotein 3 (leaf)



DATA SHEET IN PDF

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Data sheet Product citations Protocols Add review

Product Information

Immunogen Purified full length, tag cleaved, recombinant maize leaf FNR3,  UniProt: B4FUM2

Host Rabbit
Clonality Polyclonal
Purity Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Format Liquid at 1 mg/ml.
Quantity 100 ĩg
Storage Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1: 1000 (WB)
Expected | apparent MW 40,6 | 34,7 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Zea mays
Predicted reactivity Dichanthelium oligosanthes, Panicum hallii, Sorghum bicolor
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Western blot using anti-FNR3 antibodies


2 μg/well of leaf total protein of Arabidopsis thaliana wild type leaf (1), Zea mays leaf (2) were freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE. For IP, 150mM NaCL, 1% Triton X-100, 50 mM Tris-HCl (pH 8.0) and denatured with 4X SDS buffer at 95°C for 5 min. Samples were separated on 10% SDS-PAGE and blotted 1h to PVDF membrane. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 in TBS-T for 1-2h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendation.

Molecular weight of mature forms of maize L-FNRs: 
34.97 kDa (FNR1, Zea mays), 35.57 kDa (FNR2, Zea mays), 34.7 kDa (FNR3, Zea mays)

Additional information

This antibody is also detecting other maize L-FNRs: FNR2 and FNR1 in Zea mays and Arabidopsis thaliana leaf extracts, in the order of reactivity in each species.

Related products

Background

Background FNR3 | Ferredoxin NADP Reductase, isoprotein 3 (leaf) (L-FNR1) plays a key role in regulating the relative amounts of cyclic and non-cyclic electron flow to meet the demands of the plant for ATP and reducing power. Localized in chloroplast.

Product citations

Selected references Okutani et al. (2005). Three Maize Leaf ferredoxin:NADPH Oxidoreductases Vary in Subchloroplast Location, Expression, and Interaction With Ferredoxin. Plant Physiol . 2005 Nov;139(3):1451-9. doi: 10.1104/pp.105.070813.
Okutani et al. (2005). Three Maize Leaf ferredoxin:NADPH Oxidoreductases Vary in Subchloroplast Location, Expression, and Interaction With Ferredoxin. Plant Physiol. 2005 Nov;139(3):1451-9. doi: 10.1104/pp.105.070813.
immunogen: Purified full length, tag cleaved, recombinant maize leaf FNR3,  UniProt: B4FUM2

Host: Rabbit
Clonality: Polyclonal
Purity: Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Format: Liquid at 1 mg/ml.
Quantity: 100 ĩg
storage: Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: ELISA (ELISA), Western blot (WB)
recommended dilution: 1: 1000 (WB)
calculated | apparent molecular mass [kDa]: 40,6 | 34,7 kDa
Confirmed reactivity: Arabidopsis thaliana, Zea mays
predicted reactivity: Dichanthelium oligosanthes, Panicum hallii, Sorghum bicolor
Species of your interest not listed? Contact us
not reactive in: No confirmed exceptions from predicted reactivity are currently known
Picture (footer): Western blot using anti-FNR3 antibodies


2 μg/well of leaf total protein of Arabidopsis thaliana wild type leaf (1), Zea mays leaf (2) were freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE. For IP, 150mM NaCL, 1% Triton X-100, 50 mM Tris-HCl (pH 8.0) and denatured with 4X SDS buffer at 95°C for 5 min. Samples were separated on 10% SDS-PAGE and blotted 1h to PVDF membrane. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 in TBS-T for 1-2h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendation.

Molecular weight of mature forms of maize L-FNRs: 
34.97 kDa (FNR1, Zea mays), 35.57 kDa (FNR2, Zea mays), 34.7 kDa (FNR3, Zea mays)
additional information (application): This antibody is also detecting other maize L-FNRs: FNR2 and FNR1 in Zea mays and Arabidopsis thaliana leaf extracts, in the order of reactivity in each species.
background: FNR3 | Ferredoxin NADP Reductase, isoprotein 3 (leaf) (L-FNR1) plays a key role in regulating the relative amounts of cyclic and non-cyclic electron flow to meet the demands of the plant for ATP and reducing power. Localized in chloroplast.
All references: Okutani et al. (2005). Three Maize Leaf ferredoxin:NADPH Oxidoreductases Vary in Subchloroplast Location, Expression, and Interaction With Ferredoxin. Plant Physiol . 2005 Nov;139(3):1451-9. doi: 10.1104/pp.105.070813.
Okutani et al. (2005). Three Maize Leaf ferredoxin:NADPH Oxidoreductases Vary in Subchloroplast Location, Expression, and Interaction With Ferredoxin. Plant Physiol. 2005 Nov;139(3):1451-9. doi: 10.1104/pp.105.070813.

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