FNR3 | Ferredoxin NADP Reductase, isoprotein 3 (leaf)
AS20 4437 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Zea mays
DATA SHEET IN PDF| Data sheet | Product citations | Protocols | Add review |
Product Information
Immunogen
Purified full length, tag cleaved, recombinant maize leaf FNR3, UniProt: B4FUM2
Host
Rabbit
Clonality
Polyclonal
Purity
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Format
Liquid at 1 mg/ml.
Quantity
100 ĩg
Storage
Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
ELISA (ELISA), Western blot (WB)
Recommended dilution
1: 1000 (WB)
Expected | apparent MW
40,6 | 34,7 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana, Zea mays
Predicted reactivity
Dichanthelium oligosanthes, Panicum hallii, Sorghum bicolor
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
2 μg/well of leaf total protein of Arabidopsis thaliana wild type leaf (1), Zea mays leaf (2) were freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE. For IP, 150mM NaCL, 1% Triton X-100, 50 mM Tris-HCl (pH 8.0) and denatured with 4X SDS buffer at 95°C for 5 min. Samples were separated on 10% SDS-PAGE and blotted 1h to PVDF membrane. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 in TBS-T for 1-2h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendation.
Molecular weight of mature forms of maize L-FNRs:
34.97 kDa (FNR1, Zea mays), 35.57 kDa (FNR2, Zea mays), 34.7 kDa (FNR3, Zea mays)
2 μg/well of leaf total protein of Arabidopsis thaliana wild type leaf (1), Zea mays leaf (2) were freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE. For IP, 150mM NaCL, 1% Triton X-100, 50 mM Tris-HCl (pH 8.0) and denatured with 4X SDS buffer at 95°C for 5 min. Samples were separated on 10% SDS-PAGE and blotted 1h to PVDF membrane. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 in TBS-T for 1-2h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendation.
Molecular weight of mature forms of maize L-FNRs:
34.97 kDa (FNR1, Zea mays), 35.57 kDa (FNR2, Zea mays), 34.7 kDa (FNR3, Zea mays)
Additional information
This antibody is also detecting other maize L-FNRs: FNR2 and FNR1 in Zea mays and Arabidopsis thaliana leaf extracts, in the order of reactivity in each species.
Background
Background
FNR3 | Ferredoxin NADP Reductase, isoprotein 3 (leaf) (L-FNR1) plays a key role in regulating the relative amounts of cyclic and non-cyclic electron flow to meet the demands of the plant for ATP and reducing power. Localized in chloroplast.
Product citations
Selected references
Okutani et al. (2005). Three Maize Leaf ferredoxin:NADPH Oxidoreductases Vary in Subchloroplast Location, Expression, and Interaction With Ferredoxin. Plant Physiol . 2005 Nov;139(3):1451-9. doi: 10.1104/pp.105.070813.
Okutani et al. (2005). Three Maize Leaf ferredoxin:NADPH Oxidoreductases Vary in Subchloroplast Location, Expression, and Interaction With Ferredoxin. Plant Physiol. 2005 Nov;139(3):1451-9. doi: 10.1104/pp.105.070813.
Okutani et al. (2005). Three Maize Leaf ferredoxin:NADPH Oxidoreductases Vary in Subchloroplast Location, Expression, and Interaction With Ferredoxin. Plant Physiol. 2005 Nov;139(3):1451-9. doi: 10.1104/pp.105.070813.
Related products: FNR3 | Ferredoxin NADP Reductase, isoprotein 3 (leaf)
AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
This product can b...
This product can b...
From 26 €
AS20 4439 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thali...
349 €
AS20 4438 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thali...
323 €
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
10 ...
10 ...
201 €
Agrisera AB Box 57, SE-911 21 Vännäs, SWEDEN | Phone: +46(0)935 33000 | 
