FNR1 | Ferredoxin NADP Reductase, isoprotein 1 (leaf)
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10 μg/well of leaf total protein of Arabidopsis thaliana wild type leaf (1), Zea mays leaf (2) were freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE. For IP, 150mM NaCL, 1% Triton X-100, 50 mM Tris-HCl (pH 8.0) and denatured with 4X SDS buffer at 95°C for 5 min. Samples were separated on 10% SDS-PAGE and blotted 1h to PVDF membrane. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 in TBS-T for 1-2h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendation.
Molecular weight of mature forms of maize L-FNRs:
34.97 kDa (FNR1, Zea mays), 35.57 kDa (FNR2, Zea mays), 34.7 kDa (FNR3, Zea mays)
Recombinant Zea mays FNR1, 34.97 kDa (1), recombinant Zea mays FNR2, 35.57 kDa (2), recombinant Zea mays FNR3, 34, 7 kDa (3), Zea mays chloroplast fraction (4), Zea mays stroma fraction (5), Zea mays thylakoid fraction (6)
Primary antibody: 1: 500
Antibody cross reacts with other leaf maize FNR isoforms, FNR2 and FNR3.
Twachtmann et al. (2012). N-terminal Structure of Maize ferredoxin:NADP+ Reductase Determines Recruitment Into Different Thylakoid Membrane Complexes. Plant Cell. 2012 Jul;24(7):2979-91. doi: 10.1105/tpc.111.094532.
Onda et al. (2000). Differential Interaction of Maize Root ferredoxin:NADP(+) Oxidoreductase With Photosynthetic and Non-Photosynthetic Ferredoxin Isoproteins. Plant Physiol . 123(3):1037-45. doi: 10.1104/pp.123.3.1037.
Onda et al. (2000). Differential Interaction of Maize Root ferredoxin:NADP(+) Oxidoreductase With Photosynthetic and Non-Photosynthetic Ferredoxin Isoproteins. Plant Physiol. 2000 Jul;123(3):1037-45. doi: 10.1104/pp.123.3.1037.
Related products: FNR1 | Ferredoxin NADP Reductase, isoprotein 1 (leaf)
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