IKP

FNR1 | Ferredoxin NADP Reductase, isoprotein 1 (leaf)

Product no: AS20 4439
AS20 4439 | Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Arabidopsis thaliana, Zea mays
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  • Product Info
  • Immunogen: Purified recombinant maize leaf FNR1 protein UniProt: Q9SLP6, sharing homology with maize FNR2, FNR3 and Arabidopsis thaliana FNR1 Q9FKW6
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
    Format: Liquid at 1 mg/ml.
    Quantity: 200 g
    Storage: Store at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Western blot (WB)
    Recommended dilution: 1: 500 -1 : 2000 (WB)
    Expected | apparent MW: 39.3 kDa | 34.97 kDa (FNR1, Zea mays)
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Zea mays
    Predicted reactivity: Hordeum vulgare, Oryza brachyantha, Saccharum sp., Setaria italica, Sorghum bicolor
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Western blot using anti-FNR1 antibodies


    10 μg/well of leaf total protein of Arabidopsis thaliana wild type leaf (1), Zea mays leaf (2) were freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE. For IP, 150mM NaCL, 1% Triton X-100, 50 mM Tris-HCl (pH 8.0) and denatured with 4X SDS buffer at 95°C for 5 min. Samples were separated on 10% SDS-PAGE and blotted 1h to PVDF membrane. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 in TBS-T for 1-2h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendation.


    Molecular weight of mature forms of maize L-FNRs: 
    34.97 kDa (FNR1, Zea mays), 35.57 kDa (FNR2, Zea mays), 34.7 kDa (FNR3, Zea mays)


    Western blot with anti-FNR1 antibodies on various cellular fractions

    Recombinant Zea mays FNR1, 34.97 kDa (1), recombinant Zea mays FNR2, 35.57 kDa (2), recombinant Zea mays FNR3, 34, 7 kDa (3), Zea mays chloroplast fraction (4), Zea mays stroma fraction (5), Zea mays thylakoid fraction (6)

    Primary antibody: 1: 500

    Antibody cross reacts with other leaf maize FNR isoforms, FNR2 and FNR3. 
  • Additional Information
  • Additional information (application): This antibody is also detecting other maize L-FNRs, FNR2, FNR3 (reference image below) and reacts weakly with root FNR
  • Background
  • Background: Ferredoxin-NADP reductase, leaf isozyme 1 (L-FNR1) plays a key role in regulating the relative amounts of cyclic and non-cyclic electron flow to meet the demands of the plant for ATP and reducing power. Localised in the chloroplast.
  • Product Citations
  • Selected references: Twachtmann et al. (2012). N-terminal Structure of Maize ferredoxin:NADP+ Reductase Determines Recruitment Into Different Thylakoid Membrane Complexes. Plant Cell . 2012 Jul;24(7):2979-91. doi: 10.1105/tpc.111.094532.
    Twachtmann et al. (2012). N-terminal Structure of Maize ferredoxin:NADP+ Reductase Determines Recruitment Into Different Thylakoid Membrane Complexes. Plant Cell. 2012 Jul;24(7):2979-91. doi: 10.1105/tpc.111.094532.
    Onda et al. (2000). Differential Interaction of Maize Root ferredoxin:NADP(+) Oxidoreductase With Photosynthetic and Non-Photosynthetic Ferredoxin Isoproteins. Plant Physiol . 123(3):1037-45. doi: 10.1104/pp.123.3.1037.
    Onda et al. (2000). Differential Interaction of Maize Root ferredoxin:NADP(+) Oxidoreductase With Photosynthetic and Non-Photosynthetic Ferredoxin Isoproteins. Plant Physiol. 2000 Jul;123(3):1037-45. doi: 10.1104/pp.123.3.1037.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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