FNR2 | Ferredoxin NADP Reductase, isoprotein 2 (leaf)
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10 μg/well of leaf total protein of Arabidopsis thaliana wild type leaf (1), Zea mays leaf (2) were freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE. For IP, 150mM NaCL, 1% Triton X-100, 50 mM Tris-HCl (pH 8.0) and denatured with 4X SDS buffer at 95°C for 5 min. Samples were separated on 10% SDS-PAGE and blotted 1h to PVDF membrane. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 in TBS-T for 1-2h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendation.
Molecular weight of mature forms of maize L-FNRs:
34.97 kDa (FNR1, Zea mays), 35.57 kDa (FNR2, Zea mays), 34.7 kDa (FNR3, Zea mays)
Cellular distribution of maize FNR isoforms
BSC - bundle sheah cells, protein load 4 µg/well
MC- mesophyll cells, protein load 4 µg/well
Primary antibody: 1: 50 000
Anti-FNR2 Antibody cross reacts with other leaf maize FNR isoforms, FNR1 and FNR3.
Alternative names: FNR
Twachtmann et al. (2012). N-terminal Structure of Maize ferredoxin:NADP+ Reductase Determines Recruitment Into Different Thylakoid Membrane Complexes. Plant Cell. 2012 Jul;24(7):2979-91. doi: 10.1105/tpc.111.094532.
Related products: FNR2 | Ferredoxin NADP Reductase, isoprotein 2 (leaf)
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