GFP | Green fluorescent protein (VENUS) - DyLight® 594 conjugated (40 µg)
- Product Info
Immunogen: Full length, recombinat VENUS protein, expressed in E.coli, UniProt: P42212 Host: Rabbit Clonality: Polyclonal Purity:
Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight® 594.
Format: Liquid in PBS pH 7,4. Quantity: 40 µg Storage: Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years. Spin briefly the tube before use. Tested applications: Immunofluorescence (IF), Western blot (WB) Recommended dilution: To be determined by end user. Expected | apparent MW: Depends upon fusion partner
Confirmed reactivity: VENUS Not reactive in: No confirmed exceptions from predicted reactivity are currently known.
- Additional Information
Additional information: DyLight® 594 has Amax = 593 nm, Emax = 618 nm. DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
Background: Green fluorescent protein (Venus) from Aequorea victoria (Jellyfish), can be mutated to emit at different wavelengths such as blue for BFP (when Tyr-66 is replaced by His), cyan for CFP (when Tyr-66 is replaced by Trp), and yellow for YFP (when THR-203 is replaced by Tyr), It is an energy-transfer acceptor, Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer.
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