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GH | Gamma-glutamyl hydrolase

AS19 4347 | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Glycine max

GH | Gamma-glutamyl hydrolase  in the group Antibodies for Plant/Algal  / Environmental Stress / Oxidative stress at Agrisera AB (Antibodies for research) (AS19 4347)

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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen Recombinant Glycine max Gamma-glutamyl hydrolase protein, amino acids: 22-342, UniProt: P93164
Host Rabbit
Clonality Polyclonal
Purity >95%, Protein G purified, to total immunoglobulin G fraction
Format Liquid
Quantity 50 ug
Storage Store at -20°C or -80°C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW 37 kDa

Reactivity

Confirmed reactivity Glycine max
Predicted reactivity Glycine soja (Gamma-glutamyl hydrolase isoform A and B)
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example


Western blot using anti-Gamma-glutamyl hydrolase antibodies


Various amounts of Glycine max recombinant GH were loaded/well and separated on 8 % SDS-PAGE  and blotted 1h to PVDF. Blot was blocked with 5 % milk for 2h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 2.7 µg/ml for 1h/RT with agitation in PBS-T with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in  for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent, following manufacture's recommendation. 

Additional information

Additional information Preservative: 0.03% Proclin 300. Preparation contains: 50% Glycerol, 10 mM PBS, pH 7.4
Reactivity of this antibody on endogenous material remains to be determined.

Related products

Related products AS11 1811 | Anti-Gamma-glutamyl-cysteine, rabbit antibodies
AS06 186 | Anti-Gamma-ECS | Gamma glutamylcysteine synthase, rabbit antibodies

Background

Background Gamma-glutamyl hydrolase is an enzyme (EC 3.4.19.9) displaying gamma-glutamyl-peptidase activity. 
Alternative names: conjugase) (GH) (Gamma-Glu-X carboxypeptidase)

Product citations

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