IKP

GH | Gamma-glutamyl hydrolase

Product no: AS19 4347

AS19 4347 | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Glycine max

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  • Product Info
  • Immunogen: Recombinant Glycine max Gamma-glutamyl hydrolase protein, amino acids: 22-342, UniProt: P93164
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Total IgG. Protein G purified.
    Format: Liquid
    Quantity: 50 g
    Storage: Store at -20C or -80C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Western blot (WB)
    Recommended dilution: 1 : 5000 (WB)
    Expected | apparent MW: 37 kDa
  • Reactivity
  • Confirmed reactivity: Glycine max
    Predicted reactivity: Glycine soja (Gamma-glutamyl hydrolase isoform A and B)
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • application example


    Western blot using anti-Gamma-glutamyl hydrolase antibodies


    Various amounts of Glycine max recombinant GH were loaded/well and separated on 8 % SDS-PAGE  and blotted 1h to PVDF. Blot was blocked with 5 % milk for 2h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 2.7 µg/ml for 1h/RT with agitation in PBS-T with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in  for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent, following manufacture's recommendation. 

  • Additional Information
  • Additional information: Preservative: 0,03% Proclin 300, Preparation contains: 50% Glycerol, 10 mM PBS, pH 7,4
    Additional information (application): Reactivity of this antibody on endogenous material remains to be determined
  • Background
  • Background: Gamma-glutamyl hydrolase is an enzyme (EC 3.4.19.9) displaying gamma-glutamyl-peptidase activity. 
    Alternative names: conjugase) (GH) (Gamma-Glu-X carboxypeptidase)
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Poster Collection
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