GH | Gamma-glutamyl hydrolase
AS19 4347 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Glycine max
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Various amounts of Glycine max recombinant GH were loaded/well and separated on 8 % SDS-PAGE and blotted 1h to PVDF. Blot was blocked with 5 % milk for 2h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 2.7 µg/ml for 1h/RT with agitation in PBS-T with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent, following manufacture's recommendation.
Alternative names: conjugase) (GH) (Gamma-Glu-X carboxypeptidase)
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