GH | Gamma-glutamyl hydrolase

Name: GH | Gamma-glutamyl hydrolase
Brand: IKP
SKU: AS19 4347
Price: 228 EUR
Availability: InStock

AS19 4347 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Glycine max

GH | Gamma-glutamyl hydrolase in the group Antibodies Plant/Algal / Environmental Stress / Plant Oxidative Stress at Agrisera AB (Antibodies for research) (AS19 4347)

Product Information

Immunogen Recombinant Glycine max Gamma-glutamyl hydrolase protein, amino acids: 22-342, UniProt: P93164

Host Rabbit

Clonality Polyclonal

Purity Total IgG. Protein G purified.

Format Liquid

Quantity 50 µg

Storage Store at -20°C or -80°C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Western blot (WB)

Recommended dilution 1 : 5000 (WB)

Expected | apparent MW 37 kDa

Reactivity

Confirmed reactivity Glycine max

Predicted reactivity Glycine soja (Gamma-glutamyl hydrolase isoform A and B)
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example

Western blot using anti-Gamma-glutamyl hydrolase antibodies

Various amounts of Glycine max recombinant GH were loaded/well and separated on 8 % SDS-PAGE and blotted 1h to PVDF. Blot was blocked with 5 % milk for 2h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 2.7 µg/ml for 1h/RT with agitation in PBS-T with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent, following manufacture's recommendation.

Additional information

Additional information Preservative: 0,03% Proclin 300, Preparation contains: 50% Glycerol, 10 mM PBS, pH 7,4

Reactivity of this antibody on endogenous material remains to be determined

Background

Background Gamma-glutamyl hydrolase is an enzyme (EC 3.4.19.9) displaying gamma-glutamyl-peptidase activity.
Alternative names: conjugase) (GH) (Gamma-Glu-X carboxypeptidase)

Product citations

immunogen:	Recombinant Glycine max Gamma-glutamyl hydrolase protein, amino acids: 22-342, UniProt: P93164
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Total IgG. Protein G purified.
Format:	Liquid
Quantity:	50 µg
storage:	Store at -20°C or -80°C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Western blot (WB)
recommended dilution:	1 : 5000 (WB)
calculated \| apparent molecular mass [kDa]:	37 kDa
Confirmed reactivity:	Glycine max
predicted reactivity:	Glycine soja (Gamma-glutamyl hydrolase isoform A and B) Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	application example Various amounts of Glycine max recombinant GH were loaded/well and separated on 8 % SDS-PAGE and blotted 1h to PVDF. Blot was blocked with 5 % milk for 2h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 2.7 µg/ml for 1h/RT with agitation in PBS-T with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent, following manufacture's recommendation.
additional information:	Preservative: 0,03% Proclin 300, Preparation contains: 50% Glycerol, 10 mM PBS, pH 7,4
additional information (application):	Reactivity of this antibody on endogenous material remains to be determined
background:	Gamma-glutamyl hydrolase is an enzyme (EC 3.4.19.9) displaying gamma-glutamyl-peptidase activity. Alternative names: conjugase) (GH) (Gamma-Glu-X carboxypeptidase)