IKP

GltBD | NADPH-dependent glutamate synthase

Product no: AS20 4427
AS20 4427 | Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Synechocystis sp. PCC6803
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  • Product Info
  • Immunogen: Purified full length, tag cleaved, recombinant cyanobacterium, Leptolyngbya boryana (Plectonema boryanum) , glutamate synthase, UniProt: Q51583 (gltB, large subunit L.boryanum), Q51584 (gltD, small subunit L.boryanum)
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
    Format: Liquid at 4 mg/ml.
    Quantity: 200 g
    Storage: Store at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Western blot (WB)
    Recommended dilution: 1: 1000 - 1: 2000 (WB)
    Expected | apparent MW: 167.9 | 168 kDa (large subunit)
    54.2 | 54 kDa (small subunit)
  • Reactivity
  • Confirmed reactivity: Synechocystis sp. strain PCC6803
    Predicted reactivity: cyanobacteria
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Western blot using anti-NADPH-dependent glutamate synthase (GltBD)

    Soluble fraction of Synechocystis PCC6803 total cell extract freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE and denatured with 4X SDS buffer at 95°C for 5 min. Samples were separated on 10% SDS-PAGE and blotted 1h to PVDF membrane. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendation.

    Detected bands are 168 kDa (GltB, large subunit) and 54 kDa (GltD, small subunit) of NADPH-dependent glutamate synthase
  • Additional Information
  • Additional information (application): This antibody is recognizing both, large (GltB) and small (GltD) subunits of NADPH-dependent glutamate synthase
  • Background
  • Background: NADH-dependent glutamate synthase (GltBD) is involved in glutamate biosynthesis and consists of large subunit (GltB, 168 kDa) and small subunit (GltD, 54 kDa). It is required for non-photorespiratory ammonium assimilation.
  • Product Citations
  • Selected references: 38221900
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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