GltBD | NADPH-dependent glutamate synthase

Name: GltBD | NADPH-dependent glutamate synthase
Brand: IKP
SKU: AS20 4427
Price: 349 EUR
Availability: InStock

AS20 4427 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Synechocystis sp. PCC6803

GltBD | NADPH-dependent glutamate synthase in the group Antibodies Plant/Algal / Nitrogen Metabolism at Agrisera AB (Antibodies for research) (AS20 4427)

GltBD | NADPH-dependent glutamate synthase

Product Information

Immunogen Purified full length, tag cleaved, recombinant cyanobacterium, Leptolyngbya boryana (Plectonema boryanum) , glutamate synthase, UniProt: Q51583 (gltB, large subunit L.boryanum), Q51584 (gltD, small subunit L.boryanum)

Host Rabbit

Clonality Polyclonal

Purity Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.

Format Liquid at 4 mg/ml.

Quantity 200 µg

Storage Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Western blot (WB)

Recommended dilution 1: 1000 - 1: 2000 (WB)

Expected | apparent MW 167.9 | 168 kDa (large subunit)
54.2 | 54 kDa (small subunit)

Reactivity

Confirmed reactivity Synechocystis sp. strain PCC6803

Predicted reactivity cyanobacteria
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Western blot using anti-NADPH-dependent glutamate synthase (GltBD)

Soluble fraction of Synechocystis PCC6803 total cell extract freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE and denatured with 4X SDS buffer at 95°C for 5 min. Samples were separated on 10% SDS-PAGE and blotted 1h to PVDF membrane. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendation.

Detected bands are 168 kDa (GltB, large subunit) and 54 kDa (GltD, small subunit) of NADPH-dependent glutamate synthase

Additional information

This antibody is recognizing both, large (GltB) and small (GltD) subunits of NADPH-dependent glutamate synthase

Background

Background NADH-dependent glutamate synthase (GltBD) is involved in glutamate biosynthesis and consists of large subunit (GltB, 168 kDa) and small subunit (GltD, 54 kDa). It is required for non-photorespiratory ammonium assimilation.

Product citations

immunogen:	Purified full length, tag cleaved, recombinant cyanobacterium, Leptolyngbya boryana (Plectonema boryanum) , glutamate synthase, UniProt: Q51583 (gltB, large subunit L.boryanum), Q51584 (gltD, small subunit L.boryanum)
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Format:	Liquid at 4 mg/ml.
Quantity:	200 µg
storage:	Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Western blot (WB)
recommended dilution:	1: 1000 - 1: 2000 (WB)
calculated \| apparent molecular mass [kDa]:	167.9 \| 168 kDa (large subunit) 54.2 \| 54 kDa (small subunit)
Confirmed reactivity:	Synechocystis sp. strain PCC6803
predicted reactivity:	cyanobacteria Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	Soluble fraction of Synechocystis PCC6803 total cell extract freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE and denatured with 4X SDS buffer at 95°C for 5 min. Samples were separated on 10% SDS-PAGE and blotted 1h to PVDF membrane. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendation. Detected bands are 168 kDa (GltB, large subunit) and 54 kDa (GltD, small subunit) of NADPH-dependent glutamate synthase
additional information (application):	This antibody is recognizing both, large (GltB) and small (GltD) subunits of NADPH-dependent glutamate synthase
background:	NADH-dependent glutamate synthase (GltBD) is involved in glutamate biosynthesis and consists of large subunit (GltB, 168 kDa) and small subunit (GltD, 54 kDa). It is required for non-photorespiratory ammonium assimilation.