GLUC | beta-glucosidase, Biotin conjugated

AS09 450 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Prunus dulcis

Product Information

Immunogen

Native beta- glucosidase purified from almonds

Host Rabbit

Clonality Polyclonal

Purity Purified IgG

Format Lyophilized in PBS pH 7.2

Quantity 1 ml

Reconstitution For reconstitution add 1 ml of sterile destilled water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry, paraffin (IHC), Western blot (WB)

Recommended dilution 1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)

Reactivity

Confirmed reactivity Prunus dulcis

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Antibody is labelled with biotin using N-hydroxysuccinimidobiotin. Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

Biotin/IgG protein molar ration is approximately 6.2. No foreign proteins are added.

Related products

AS09 449 | Anti-beta glucosidase (total IgG), rabbit antibodies

AS09 451 | Anti-beta glucosidase (affinity purified), rabbit antibodies

Plant and algal protein extraction buffer

Secondary antibodies

Background

beta-glucosidase (EC=3.2.1.21) is an enzyme which catalyzes the hydrolysis of terminal non-reducing residues in beta-D-glucosides with release of glucose acting upon upon β1->4 bonds linking two glucose or glucose-substituted molecules.