Name:
Phone:
E-mail:
Message
AGO set
Tag Antibody Set 

SPS | Sucrose phosphate synthase, global

AS03 035A |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: [global antibody] for di and monocots  |  cellular [compartment marker] of cytoplasm for photosynthetic tissues.

SPS | Sucrose phosphate synthase, global in the group Antibodies for Plant/Algal  / Global Antibodies at Agrisera AB (Antibodies for research) (AS03 035A)

PRODUCT INFORMATION IN PDF


Datasheet Product citations Protocols Customer reviews

 

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from conserved region within plant SPS protein sequences, including Arabidopsis thaliana isoforms 1F Q94BT0, 2F, 3F and 4F. Oryza sativa Q67WN8, Solanum tuberosum Q43845

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000-1 : 5000 (WB)
Expected | apparent MW

120 | 120-130 kDa (fragments of 30/90 kDa may be detected)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Colobanthus quitensis Kunt Bartl, Hordeum vulgare, Lycopersicum esculentum, Lycopersicum penelli, Pinus strobus, Solanum tuberosum, Triticum aestivum, Pinus strobus, Zea mays
Predicted reactivity Brassica napus, Citrus sinensis, Glycine max, Nicotiana tabacum, Oryza sativa, Physcomitrella patens, Populus balsamifera, Robinia pseudoacaci, Ricinus communis, Saccharum officinarum, Solanum lycopersicum, Theobroma cacao, Vicia faba, Vitis vinifera
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example

10 µg of total leaf protein from (1) A.thaliana,(3) Zea mays and (4) Hordeum vulgare extracted with PEB (AS08 300) as well as 10 µg cytosolic protein from (2) A.thaliana were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1.5h (30V) to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-SPS (AS03 035A, 1:2000, 1h) and secondary anti-rabbit (1:20 000, 1 h) antibody (HRP conjugated) in TBS-T containing 2% low fat milk powder.  Antibody incubations where followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with chemiluminescent detection reagent, using a Fuji LAS-3000 CCD (90s, high sensitivity).

Additional information

Peptide used to elicit anti-SPS antibodies is perfectly conserved in all isoforms of SPS in plants.

Related products

Related products
AS06 185 | Anti-SPS | Sucrose phosphate synthase (maize), rabbit antibodies
AS03 035-HRP | Anti-SPS | Sucrose phosphate synthase, global (40 µg, HRP-conjugated), rabbit antibodies
AS03 035-ALP | Anti-SPS | Sucrose phosphate synthase, global (40 µg, ALP-conjugated), rabbit antibodies
AS15 2995 | Anti-SPSA1 | Sucrose phosphate synthase isoform A1, rabbit antibodies
AS15 2996 | Anti-SPSA2 | Sucrose phosphate synthase isoform A1, rabbit antibodies
AS15 2997 | Anti-SPSC | Sucrose phosphate synthase isoform C, rabbit antibodies

Plant and algal protein extraction buffer

Background

Background

SPS (sucrose phosphate synthase, EC 2.4.1.14) is the key enzyme of carbon flux into sucrose fixation in plants. It catalyzes the synthesis of sucrose-phosphate from UDP-glucose and fructose-6-phosphate predominantly in the cytosol of sucrose-source leaf tissue.

Product citations

Selected references Zhang et al. (2014). Heterologous expression of AtPAP2 in transgenic potato influences carbon metabolism and tuber development. FEBS Lett. 2014 Aug 27. pii: S0014-5793(14)00621-8. doi: 10.1016/j.febslet.2014.08.019.

Related products: SPS | Sucrose phosphate synthase, global

AS16 ECL-SN-10 | low pico to mid femtogram and extreme low femtogram detection
15 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
181 €
AS03 035-ALP |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: [g...
417 €
AS03 035-HRP |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: [g...
417 €