Goat anti-Biotin, DyLight® 650 conjugated

AS12 2228 | Clonality: Polyclonal | Host: Goat | Reactivity: Biotin

Product Information

Immunogen

purified biotin, whole molecule

Host Goat

Clonality Polyclonal

Purity Immunogen affinity purified in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. Contains 0.05 % sodium azide.

Format Lyophilized

Quantity 1 mg

Reconstitution For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Recommended dilution 1 : 20-1 : 2000 for most applications

Reactivity

Not reactive in

Application examples

Additional information

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

This antibody reacts with Biotin

This antibody does not react with free BSA or free KLH

Background

Goat anti-Biotin - DyLight® 650 Conjugated is a secondary antibody conjugated to DyLight® 650, which binds to biotin in immunological assays.

DyLight® 650 has Amax = 652 nm, Emax = 672 nm. Antibodies are affinity purified using solid phase biotin.

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Product citations

Selected references Ainla et al. (2013). Lab on a Biomembrane: rapid prototyping and manipulation of 2D fluidic lipid bilayers circuits. Sci Rep. 2013 Sep 25;3:2743. doi: 10.1038/srep02743.

Clonality:	Polyclonal
Format:	Lyophilized
Host:	Goat
immunogen:	purified biotin, whole molecule
Purity:	Immunogen affinity purified in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. Contains 0.05 % sodium azide.
Quantity:	1 mg
recommended dilution:	1 : 20-1 : 2000 for most applications
Reconstitution:	For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
storage:	Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
not reactive in:
additional information:	Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative. This antibody reacts with Biotin This antibody does not react with free BSA or free KLH
additional information (application):
All references:	Ainla et al. (2013). Lab on a Biomembrane: rapid prototyping and manipulation of 2D fluidic lipid bilayers circuits. Sci Rep. 2013 Sep 25;3:2743. doi: 10.1038/srep02743.
Picture (footer):
background:	Goat anti-Biotin - DyLight® 650 Conjugated is a secondary antibody conjugated to DyLight® 650, which binds to biotin in immunological assays. DyLight® 650 has Amax = 652 nm, Emax = 672 nm. Antibodies are affinity purified using solid phase biotin. DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

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