Goat anti-Biotin, DyLightŪ 650 conjugated
AS12 2228 | Clonality: Polyclonal | Host: Goat | Reactivity: Biotin
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Product Information
Immunogen
purified biotin, whole molecule
Host
Goat
Clonality
Polyclonal
Purity
Affinity purified biotin
Format
Lyophilized
Quantity
1 mg
Reconstitution
For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Recommended dilution
1 : 20-1 : 2000 for most applications
Reactivity
Not reactive in
Application examples
Application examples
Additional information
Additional information
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.
This antibody reacts with Biotin
This antibody does not react with free BSA or free KLH
Background
Background
Goat anti-Biotin - DyLight® 650 Conjugated is a secondary antibody conjugated to DyLight® 650, which binds to biotin in immunological assays.
DyLight® 650 has Amax = 652 nm, Emax = 672 nm. Antibodies are affinity purified using solid phase biotin.
DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product citations
Selected references
Ainla et al. (2013). Lab on a Biomembrane: rapid prototyping and manipulation of 2D fluidic lipid bilayers circuits. Sci Rep. 2013 Sep 25;3:2743. doi: 10.1038/srep02743.
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